DNA replication and genome stability

Section edited by Stuart MacNeill

This section will consider submissions focusing on molecular mechanisms of DNA replication, DNA repair, recombination and transposition in bacteria, archaea, eukaryotes and viruses.

  1. Research article

    Physical shearing imparts biological activity to DNA and ability to transmit itself horizontally across species and kingdom boundaries

    We have recently reported that cell-free DNA (cfDNA) fragments derived from dying cells that circulate in blood are biologically active molecules and can readily enter into healthy cells to activate DNA damage...

    Gorantla Venkata Raghuram, Deepika Gupta, Siddharth Subramaniam, Ashwini Gaikwad, Naveen Kumar Khare, Malcolm Nobre, Naveen Kumar Nair and Indraneel Mittra

    BMC Molecular Biology 2017 18:21

    Published on: 9 August 2017

  2. Research article

    hSSB1 associates with and promotes stability of the BLM helicase

    Maintenance of genome stability is critical in human cells. Mutations in or loss of genome stability pathways can lead to a number of pathologies including cancer. hSSB1 is a critical DNA repair protein functi...

    Laura V. Croft, Nicholas W. Ashton, Nicolas Paquet, Emma Bolderson, Kenneth J. O’Byrne and Derek J. Richard

    BMC Molecular Biology 2017 18:13

    Published on: 15 May 2017

  3. Research article

    Immunoglobulin T from sea bass (Dicentrarchus labrax L.): molecular characterization, tissue localization and expression after nodavirus infection

    Immunoglobulins (Igs) are fundamental components of the adaptive immune system of vertebrates, with the IgT/IgZ isotype specific of Teleosts. In this paper we describe the identification of an IgT heavy chain ...

    Francesco Buonocore, Valentina Stocchi, Noelia Nunez-Ortiz, Elisa Randelli, Marco Gerdol, Alberto Pallavicini, Angelo Facchiano, Chiara Bernini, Laura Guerra, Giuseppe Scapigliati and Simona Picchietti

    BMC Molecular Biology 2017 18:8

    Published on: 15 March 2017

  4. Methodology article

    Methylated site display (MSD)-AFLP, a sensitive and affordable method for analysis of CpG methylation profiles

    It has been pointed out that environmental factors or chemicals can cause diseases that are developmental in origin. To detect abnormal epigenetic alterations in DNA methylation, convenient and cost-effective ...

    Toshiki Aiba, Toshiyuki Saito, Akiko Hayashi, Shinji Sato, Harunobu Yunokawa, Toru Maruyama, Wataru Fujibuchi, Hisaka Kurita, Chiharu Tohyama and Seiichiroh Ohsako

    BMC Molecular Biology 2017 18:7

    Published on: 9 March 2017

  5. Research article

    Novel insight into the composition of human single-stranded DNA-binding protein 1 (hSSB1)-containing protein complexes

    Single-stranded DNA-binding proteins are essential cellular components required for the protection, metabolism and processing of single-stranded DNA. Human single-stranded DNA-binding protein 1 (hSSB1) is one ...

    Nicholas W. Ashton, Dorothy Loo, Nicolas Paquet, Kenneth J. O’Byrne and Derek J. Richard

    BMC Molecular Biology 2016 17:24

    Published on: 9 December 2016

  6. Review

    Nucleophosmin: from structure and function to disease development

    Nucleophosmin (NPM1) is a critical cellular protein that has been implicated in a number of pathways including mRNA transport, chromatin remodeling, apoptosis and genome stability. NPM1 function is a critical ...

    Joseph K. Box, Nicolas Paquet, Mark N. Adams, Didier Boucher, Emma Bolderson, Kenneth J. O’Byrne and Derek J. Richard

    BMC Molecular Biology 2016 17:19

    Published on: 24 August 2016

  7. Research article

    DNA double-strand break repair is impaired in presenescent Syrian hamster fibroblasts

    Studies of DNA damage response are critical for the comprehensive understanding of age-related changes in cells, tissues and organisms. Syrian hamster cells halt proliferation and become presenescent after sev...

    Ljudmila Solovjeva, Denis Firsanov, Anastasia Vasilishina, Vadim Chagin, Nadezhda Pleskach, Andrey Kropotov and Maria Svetlova

    BMC Molecular Biology 2015 16:18

    Published on: 12 October 2015

  8. Research article

    Insight into the cellular involvement of the two reverse gyrases from the hyperthermophilic archaeon Sulfolobus solfataricus

    Reverse gyrases are DNA topoisomerases characterized by their unique DNA positive-supercoiling activity. Sulfolobus solfataricus, like most Crenarchaeota, contains two genes each encoding a reverse gyrase. We sho...

    Mohea Couturier, Anna H Bizard, Florence Garnier and Marc Nadal

    BMC Molecular Biology 2014 15:18

    Published on: 9 September 2014

  9. Research article

    Visualization of the physical and functional interaction between hMYH and hRad9 by Dronpa bimolecular fluorescence complementation

    Human MutY glycosylase homolog (hMYH), a component of the base excision repair pathway, is responsible for the generation of apurinic/apyrimidinic sites. Rad9-Rad1-Hus1 (9-1-1) is a heterotrimeric protein comp...

    Lia Agustina, Soo-Hyun Hahm, Se Hee Han, An Hue Vy Tran, Ji Hyung Chung, Jong-Hwa Park, Jin Woo Park and Ye Sun Han

    BMC Molecular Biology 2014 15:17

    Published on: 15 August 2014

  10. Methodology article

    Genetic transformation of Fusarium avenaceum by Agrobacterium tumefaciens mediated transformation and the development of a USER-Brick vector construction system

    The plant pathogenic and saprophytic fungus Fusarium avenaceum causes considerable in-field and post-field losses worldwide due to its infections of a wide range of different crops. Despite its significant impact...

    Lisette Quaade Sørensen, Erik Lysøe, Jesper Erup Larsen, Paiman Khorsand-Jamal, Kristian Fog Nielsen and Rasmus John Normand Frandsen

    BMC Molecular Biology 2014 15:15

    Published on: 22 July 2014

  11. Review

    VprBP (DCAF1): a promiscuous substrate recognition subunit that incorporates into both RING-family CRL4 and HECT-family EDD/UBR5 E3 ubiquitin ligases

    The terminal step in the ubiquitin modification system relies on an E3 ubiquitin ligase to facilitate transfer of ubiquitin to a protein substrate. The substrate recognition and ubiquitin transfer activities o...

    Tadashi Nakagawa, Koushik Mondal and Patrick C Swanson

    BMC Molecular Biology 2013 14:22

    Published on: 13 September 2013

  12. Research article

    Pseudo attP sites in favor of transgene integration and expression in cultured porcine cells identified by streptomyces phage phiC31 integrase

    Phage PhiC31 integrase integrates attB-containing plasmid into pseudo attP site in eukaryotic genomes in a unidirectional site-specific manner and maintains robust transgene expression. Few studies, however, e...

    Yanzhen Bi, Ximei Liu, Long Zhang, Changwei Shao, Zhuo Ma, Zaidong Hua, Liping Zhang, Li Li, Wenjun Hua, Hongwei Xiao, Qingxin Wei and Xinmin Zheng

    BMC Molecular Biology 2013 14:20

    Published on: 8 September 2013

  13. Research article

    Three-stage biochemical selection: cloning of prototype class IIS/IIC/IIG restriction endonuclease-methyltransferase TsoI from the thermophile Thermus scotoductus

    In continuing our research into the new family of bifunctional restriction endonucleases (REases), we describe the cloning of the tsoIRM gene. Currently, the family includes six thermostable enzymes: TaqII, Tth11...

    Piotr M Skowron, Jolanta Vitkute, Danute Ramanauskaite, Goda Mitkaite, Joanna Jezewska-Frackowiak, Joanna Zebrowska, Agnieszka Zylicz-Stachula and Arvydas Lubys

    BMC Molecular Biology 2013 14:17

    Published on: 6 August 2013

  14. Research article

    Drosophila MOF controls Checkpoint protein2 and regulates genomic stability during early embryogenesis

    In Drosophila embryos, checkpoints maintain genome stability by delaying cell cycle progression that allows time for damage repair or to complete DNA synthesis. Drosophila MOF, a member of MYST histone acetyl tra...

    Sreerangam NCVL Pushpavalli, Arpita Sarkar, M Janaki Ramaiah, Debabani Roy Chowdhury, Utpal Bhadra and Manika Pal-Bhadra

    BMC Molecular Biology 2013 14:1

    Published on: 24 January 2013

  15. Research article

    Directional telomeric silencing and lack of canonical B1 elements in two silencer Autonomously Replicating Sequences in S. cerevisiae

    Autonomously Replicating Sequences (ARS) in S. cerevisiae serve as origins of DNA replication or as components of cis-acting silencers, which impose positional repression at the mating type loci and at the telome...

    Patricia Chisamore-Robert, Samantha Peeters, Kristina Shostak and Krassimir Yankulov

    BMC Molecular Biology 2012 13:34

    Published on: 16 November 2012

  16. Methodology article

    A MultiSite GatewayTM vector set for the functional analysis of genes in the model Saccharomyces cerevisiae

    Recombinatorial cloning using the GatewayTM technology has been the method of choice for high-throughput omics projects, resulting in the availability of entire ORFeomes in GatewayTM compatible vectors. The Multi...

    Astrid Nagels Durand, Tessa Moses, Rebecca De Clercq, Alain Goossens and Laurens Pauwels

    BMC Molecular Biology 2012 13:30

    Published on: 20 September 2012