- Research article
- Open Access
Insight into the cellular involvement of the two reverse gyrases from the hyperthermophilic archaeon Sulfolobus solfataricus
- Mohea Couturier1, 2, 3, 4,
- Anna H Bizard1, 2, 5,
- Florence Garnier†1, 2Email author and
- Marc Nadal†1, 2, 6Email author
© Couturier et al.; licensee BioMed Central Ltd. 2014
- Received: 15 July 2014
- Accepted: 27 August 2014
- Published: 9 September 2014
Reverse gyrases are DNA topoisomerases characterized by their unique DNA positive-supercoiling activity. Sulfolobus solfataricus, like most Crenarchaeota, contains two genes each encoding a reverse gyrase. We showed previously that the two genes are differently regulated according to temperature and that the corresponding purified recombinant reverse gyrases have different enzymatic characteristics. These observations suggest a specialization of functions of the two reverse gyrases. As no mutants of the TopR genes could be obtained in Sulfolobales, we used immunodetection techniques to study the function(s) of these proteins in S. solfataricus in vivo. In particular, we investigated whether one or both reverse gyrases are required for the hyperthermophilic lifestyle.
For the first time the two reverse gyrases of S. solfataricus have been discriminated at the protein level and their respective amounts have been determined in vivo. Actively dividing S. solfataricus cells contain only small amounts of both reverse gyrases, approximately 50 TopR1 and 125 TopR2 molecules per cell at 80°C. S. solfataricus cells are resistant at 45°C for several weeks, but there is neither cell division nor replication initiation; these processes are fully restored upon a return to 80°C. TopR1 is not found after three weeks at 45°C whereas the amount of TopR2 remains constant. Enzymatic assays in vitro indicate that TopR1 is not active at 45°C but that TopR2 exhibits highly positive DNA supercoiling activity at 45°C.
The two reverse gyrases of S. solfataricus are differently regulated, in terms of protein abundance, in vivo at 80°C and 45°C. TopR2 is present both at high and low temperatures and is therefore presumably required whether cells are dividing or not. By contrast, TopR1 is present only at high temperature where the cell division occurs, suggesting that TopR1 is required for controlling DNA topology associated with cell division activity and/or life at high temperature. Our findings in vitro that TopR1 is able to positively supercoil DNA only at high temperature, and TopR2 is active at both temperatures are consistent with them having different functions within the cells.
- Low temperature
DNA topoisomerases are enzymes responsible for changing the DNA topological state. They are necessarily involved in all DNA processes (transcription, replication, recombination, repair and chromosome segregation) and the resulting appropriate topoisomerase activity modifies the DNA linking number locally and thereby eliminate excess of negative and positive supercoils generated upstream and downstream from the corresponding machinery . Topoisomerases are classified as type I or type II according to whether the transient break in the DNA during their activity is a single-strand (type I) or double-strand (type II) [2, 3]. They are further classified as type IA or IB and type IIA or IIB according to the presence of particular motifs in the amino-acid sequence .
Reverse gyrase is a particular type IA topoisomerase and it is the only known DNA topoisomerase to introduce positive supercoils into DNA [4–9]. Reverse gyrase was initially discovered in hyperthermophilic and thermophilic Archaea and Bacteria[10, 11]. It was further reported that reverse gyrase was the marker of hyperthermophily and that the corresponding gene(s) may be essential for life at high temperature [12, 13]. The positive supercoiling activity of reverse gyrase was proposed to stabilize the DNA duplex against denaturation in extreme temperature environments [9, 14], and thereby avoid DNA melting. Positive supercoiling of DNA prevents formation of open complexes  as demonstrated by in vitro assays of Sulfolobus DNA transcription . Homeostatic control of DNA supercoiling involving reverse gyrase has been suggested in hyperthermophilic archaea , as it was previously reported for mesophilic bacteria [18, 19]. Reverse gyrase acts in vitro as a heat-protective DNA chaperone, independently of its supercoiling activity . The helicase-topoisomerase IA chimeric structure of the reverse gyrase [9, 21, 22] is reminiscent of the physical and functional interaction between the RecQ-like protein and topoisomerase III. This protein pair is found in Bacteria and Eukarya, and is involved in the DNA repair and recombination needed for genome stability [23–25]. It has therefore been suggested that the reverse gyrase in hyperthermophilic archaea has a role in the maintenance of genome stability [9, 26]. Reverse gyrase efficiently anneals complementary single-stranded circles and introduces positive supercoils into DNA containing a bubble and may thus act as a renaturase, contributing to the genome stability by eliminating impaired regions [27, 28]. There is indeed diverse evidence that reverse gyrase is involved in recombination and repair. It is specifically recruited to DNA after UV irradiation . The positive supercoiling reaction of reverse gyrase in vitro is stimulated by the single-strand DNA binding protein (SSB) , a protein that binds to single-strand DNA to prevent its premature annealing during various DNA metabolism processes including replication, recombination and repair ; a functional interaction between these two proteins has been demonstrated in vivo in the presence of DNA . SSB also enhances the binding and cleavage of UV-irradiated substrates by reverse gyrase, further implicating reverse gyrase in DNA repair . Reverse gyrase inhibits the activity of the translesion DNA polymerase PolY/Dpo4 in vitro, possibly thereby preventing the potential high mutational effect of PolY/Dpo4 . Finally, reverse gyrase shows unwinding activity of substrates containing helical junctions, consistent with its involvement in recombination and repair .
A gene encoding a reverse gyrase has been also discovered in some moderately thermophilic bacteria: for example in Nautilia profundicola, which grows optimally at 45°C , the expression of this gene increases substantially at higher temperature. This may confer a selective advantage for such organisms which live close to hydrothermal vents and are therefore subject to frequent and rapid temperature fluctuations . Possibly, reverse gyrase may be important for thermoadaptation rather than the hyperthermophilic lifestyle as such. The situation seems to differ between hyperthermophilic organisms with one and those with two reverse gyrase genes. Thermococcus kodakaraensis is a hyperthermophilic organism belonging to the Euryarchaeota phylum; it has a single reverse gyrase gene that was shown to be not essential for hyperthermophilic life, except at temperatures above 90°C . By contrast, in the crenarchaeon Sulfolobus islandicus, both topR1 and topR2 genes were recently demonstrated to be essential . Thus, the two reverse gyrase genes in Sulfolobales, and possibly in all Crenarchaeota containing two, seem to be linked either to the hyperthermophilic lifestyle and/or to other essential functions.
Reverse gyrases clearly have several functions in the cell, probably involving interactions with different partners according to the cellular process. The redundancy of reverse gyrase genes in most members of the Crenarchaeota phylum strongly suggests specialization of the two reverse gyrases with TopR1 and TopR2 having different functions. The topR1 and topR2 genes in S. solfataricus P2 are differently regulated, with different expression patterns according to the growth phase and temperature, and TopR1 is probably involved in the control of the topological state of DNA . Experiments in vitro with the two purified recombinant reverse gyrases from S. solfataricus showed that they exhibit different enzymatic characteristics and in particular different behaviors with respect to temperature .
As both genes are essential in Sulfolobales, we looked for culture conditions revealing differential regulation of the two enzymes to study further their respective roles. We determined the lowest temperature at which only one of the two reverse gyrases exhibits significant enzymatic activity in vitro. Then, we tested whether S. solfataricus P2 cells at this temperature contain one or both reverse gyrases, to assess whether one or both reverse gyrases are tightly linked to the hyperthermophilic lifestyle. We report that TopR1 is not active at 45°C whereas TopR2 exhibits significant positive supercoiling activity at this temperature. We also report for the first time the number of reverse gyrase molecules per cell: S. solfataricus contains approximately 50 molecules of TopR1 and 125 molecules of TopR2 per cell when cells are actively dividing at 80°C. After three weeks at 45°C, there was no cell division nor replication initiation; the abundance of TopR1 was very much lower than at 80°C whereas that of TopR2 is largely unaffected. The cultures were returned to 80°C, and growth ability and replication activity are fully restored and the amounts of TopR1 characteristic of actively dividing cells were recovered. These quantitative findings contribute to elucidating the different roles of reverse gyrase.
TopR2, but not TopR1, remains active at 45°C
S. solfataricus cells preserve their membrane integrity at 45°C, but cells do not divide
Control cells cultured at 80°C and cells transferred to 45°C were examined by phase contrast microscopy: that both presented the same round shape (Figure 2B). Average cell sizes were calculated from measurements of at least 200 cells in each condition. In both conditions, the cells were similarly small: 1.60 μm ± 0.30 for control cells at 80°C and 1.53 μm ± 0.27 for cells transferred to 45°C, independent of the time at which the cells are collected (Figure 2B, panels b-d compared with panel a). This is consistent with a previous report that Sulfolobus cell size is not affected by transfer to room temperature or even ice-water .
When cells are exposed to an environmental condition different from their optimal growth conditions, cell membrane integrity may be affected. We used the LIVE/DEAD® Bac Light™ Bacterial Viability Kit , which has been validated for archaea  to test membrane integrity. As expected, the exponentially growing cultures at 80°C contained few damaged cells (Figure 2C, panel a); after transfer from 80°C to 45°C and incubation for 21 days, the numbers of cells with preserved membrane integrity were similar to control values (Figure 2C, panels b-d). This indicates that the cells do not lyse at this low temperature, and are resistant to it for a long period.
We used flow cytometry to investigate cell size and cell composition (forward light scattering; FSC) and cell distribution according to DNA content. Cells transferred to 45°C became slightly more heterogeneous, exhibiting a wider range of FSC than control cells (Figure 2D, b, c and d compared with a). There was a symmetric decrease of the FSC range for cells transferred back to 80°C after three weeks at 45°C (Figure 2D, e compared with b, c and d). As microscopy indicated that there was no significant change in the cell size at any time during the experiment, regardless the direction of temperature shift (from 80°C to 45°C or from 45°C to 80°C), the symmetric and reproducible variation of the FSC parameter presumably reflects a change in cell composition. Indeed, the protein concentration declined when cells were transferred to 45°C to half that in 80°C control cells. This probably reflects a change in the balance between degradation, stability and basal synthesis for a particular set of proteins. Changes in the lipid membrane composition is also a plausible explanation because the number of cyclopentane rings in the tetraether lipids of Sulfolobale membranes varies with temperature . The composition and/or the properties of the flexible cell wall may also be modified by the temperature change and affect the FSC.
The 80°C control cells could be separated into three sub-populations according to the DNA content (Figure 2D, panel a) as previously described : a minor population containing one genome equivalent; a significant population with a DNA content between one and two genome equivalents; and the largest cell population containing two genome equivalents. The proportions of the different populations in S. solfataricus cultures growing exponentially at 80°C were consistent with the relative lengths of cell cycle phases as previously reported for actively dividing Sulfolobus cells . This distribution was drastically modified after a prolonged incubation at 45°C: the cytograms after 7, 14 and 21 days at 45°C evidence the disappearance of cells with a DNA content between one and two genome equivalents; cells containing one genome continue to constitute a small part of the cell population; and the proportion of cells with two genome equivalents increases even further (Figure 2D, panels b-d). The absence of cells with intermediate amounts of DNA reflects the absence of active replication. As the cell density remains constant, it is reasonable to correlate the absence of replication with the absence of cell division at 45°C. The disappearance of cells with intermediate amounts of DNA cannot be explained by degradation of partially replicated DNA because this process would imply either a large increase of the cell population harboring one genome or the lysis of these cells. These two possibilities are clearly not in accordance with our results. Thus, we conclude that the cells having initiated replication, finish their replication, leading cells with two genome equivalents. This completion of replication may be slow, that is consistent with the reduced processivity of the DNA replicases PolB1/Dpo1 and PolY/Dpo4, shown in vitro with decreasing temperatures [39, 40]. Further evidence for this is that the cell distribution 48 hours after transfer to 45°C is similar to that of control cells except that the population of cells with an intermediate DNA content is slightly but significantly shifted towards a DNA content between 1.5 and 2 equivalent genomes and away from 1 to 1.5 genomes (data not shown). Moreover, the cell density increases slightly during these first two days, consistent with residual cell division. We cannot exclude the possibility that there may have been initiation of replication during the first few days after transfer. It has been shown that during the cell cycle of Sulfolobus cultivated at 80°C, a new round of replication can occur only when cell division has been completed . However, we cannot completely exclude the possibility that the absence of cell division was as consequence of there having been no replication activity upon prolonged incubation at 45°C. In any case, the absence of cells with an intermediate DNA content after seven days at 45°C clearly indicates that no new replication started during prolonged incubation at 45°C.
When cells maintained at 45°C for 21 days were transferred back to 80°C, there was a transient small decrease of the OD600nm within a couple of hours (Figure 2A). The OD600nm increased progressively thereafter and the growth curve of the cultures transferred from 45°C to 80°C was similar to that for control cultures at 80°C (Figure 2A). Cell size did not change following the return to 80°C (1.6 μm ± 0.27) (Figure 2B, panel e) and the proportion of damaged cells remained very low, and similar to control values (Figure 2C, panel e). In flow cytometry, the cytogram of cells transferred back to 80°C and collected 24 hours after the up-shift was similar to that of the 80°C control cells (Figure 2D, panel e and panel a, respectively). Indeed, the presence of cells with an intermediate DNA content is fully restored, indicating that normal replication activity was recovered. Hence, the Sulfolobus cells maintained at 45°C retained their ability to grow again actively at 80°C even after three weeks at the low temperature. A similar phenomenon has been reported for Sulfolobus cells kept for a short time at room temperature and transferred back to their optimal growth temperature .
In conclusion, S. solfataricus cells are resistant to long periods at 45°C and are able to recover a normal cell activity, i.e. cell division and replication, when they are transferred back to 80°C.
Quantification of TopR1 and TopR2 per S. solfataricus cell
Number of TopR1 and TopR2 per S. solfataricus cell at 80°C
This is the first study reporting the specific detection of two reverse gyrases within the same organism. The immunodetection signal obtained for cells growing exponentially at 80°C (Figure 4A and B, lane 1) corresponded to 54 ± 6 molecules of TopR1 and 127 ± 18 molecules of TopR2 per cell (Figure 4C). These are very low values, but nevertheless in agreement with the very small amounts of the corresponding transcripts reported previously .
It has been shown that reverse gyrase has a heat-protective DNA chaperone activity in vitro. This activity requires a high protein/DNA mass ratio, of at least 10 . The results we report here indicate a total of approximately 180 molecules of reverse gyrase per cell; if reverse gyrases are regularly distributed along the chromosome, this corresponds to a protein/DNA mass ratio approximately 38-fold lower than required for the in vitro chaperone activity. These observations can be reconciled by putative recruitment of reverse gyrase to, for example, sites of damaged DNA as previously discussed [9, 29, 49]. Similarly, our evidence that there is little reverse gyrase (either TopR1 or TopR2) in each cell is discordant with the requirement for a saturating concentration of the protein for the unwinding activity of TopR1 of four-way DNA junctions .
By contrast, our estimations of the amounts of reverse gyrase in vivo are consistent with the renaturase activity evidenced in in vitro. Indeed the introduction of positive supercoils into DNA containing a bubble of denaturation or the annealing of complementary single strand DNA circles have been evidenced in vitro with low protein/DNA mass ratios (0.5 and 0.8, respectively) . These activities are in agreement with thermoprotection of DNA by reverse gyrase: its positive supercoiling activity limits the formation of single-strand regions at high temperature . All in vitro experiments have been performed with DNA substrate devoid of any other bound protein; however, in vivo, DNA is obviously stabilized by the binding of chromatin proteins, such as Sul7d. The activities of reverse gyrase evidenced in vitro may be modulated by DNA binding proteins. Indeed, Sul7d inhibits reverse gyrase activity , and the single-strand binding protein, SSB, stimulates both DNA binding and the positive supercoiling of reverse gyrase .
Loss of TopR1 from S. solfataricus cells maintained at 45°C
When cells were transferred from 80°C to 45°C for three weeks, the amount of TopR2 per cell remained approximately constant throughout the period at 45°C (Figure 4B and C, conditions 2-4) suggesting either particularly high stability and/or a basal synthesis. The concentration of TopR1 decreased (Figure 4A and C, conditions 2-4): the number of TopR1 per cell declined by two thirds after 7 days, four fifths after 14 days, and to close to the detection threshold after 21 days at 45°C (Figure 4C, conditions 2-4). There was no cell division at 45°C such that the cell number remained unchanged over the three weeks at 45°C, so the loss of TopR1 was necessarily due to a specific but slow degradation. The protein concentration in the crude extracts from cells transferred to 45°C was systematically half that in extracts from 80°C control cells. Consequently, the number of TopR1 molecules per unit protein declined even more than the number per cell, whereas the number of TopR2 molecules per unit protein doubled. Thus, the amounts of the two reverse gyrases were regulated differently in this long-term down-shift experiment.Cells maintained at 45°C for 21 days were able to resume active cell division when shifted back to 80°C. We assayed the two reverse gyrases 24 hours after the shift back to 80°C. The concentration of TopR1 (molecules per cell) returned to baseline control values and that of TopR2 remained unchanged (Figure 4C, condition 5 compared with condition 1). Thus, the ability of the cells to divide correlated with there being appropriate amounts of both TopR1 and TopR2.
The amounts of TopR1 and TopR2 did not change detectably within the first 6 hours following the transfer from 80°C to 45°C (data not shown). The loss of TopR1 observed was slow, and may therefore have been related to the reduced cell activity rather than directly to the temperature change itself. We found that TopR1 is not active at low temperature in vitro, consistent with its loss from cultures at low temperature. When the cell activity is reduced, DNA transaction processes are less active, and therefore rigorous control of the topological state of DNA is less critical. At 45°C there was no cell division and no replication, and therefore few or no topological modification of the DNA, so presumably TopR1 is not needed. This implies that TopR1 is the topoisomerase mostly responsible for the DNA topological state in S. solfataricus. However, it is also possible that, at low temperature, TopR2 complements the TopR1 deficiency. TopR2 exhibits DNA positive supercoiling activity, which may be sufficient for the residual DNA topological regulation at low temperature. In cells growing at high temperature, TopR1 is presumably required to resolve the frequent modifications of DNA topological state triggered by the cell division activity and enhanced by the high temperature itself.
TopR2 is present both at high temperature (actively dividing cells) and low temperature (non-dividing cells) and it is active at 45°C in vitro (Figure 1B and C). The resumption of normal cell activity following the shift back to 80°C after three weeks at 45°C reveals that genome stability was preserved. Although there is little rigorous evidence, reverse gyrase has long been proposed to be involved in maintenance of genome stability [9, 26]. As only TopR2 is the only reverse gyrase maintained in cells at 45°C, it may have this function either by stabilizing particular regions of the DNA or by participating in DNA metabolism pathways. After a long period at 45°C, cultures contain few or no cells with between 1 and 2 genome equivalent, although such cells with 1-2 genome equivalent are observed during the 48 h following the transfer to 45°C. TopR2 may contribute to the residual replication activity in cells with 1-2 genome equivalent, such that they progress to containing a whole number (two) of genomes. Transcriptional analysis has shown that the abundance of the topR2 transcript increases during the G1/S transition phase, suggesting an implication of TopR2 in replication . At 45°C, TopR2 may have short-term functions in both genome stability and replication; subsequently, only the function contributing to genome stability appears to be relevant in the long term as there is no new replication.
TopR1 from S. solfataricus inhibits the in vitro activity of the translesion DNA PolY/Dpo4, a DNA polymerase involved in response to DNA damage . TopR1 and PolY/Dpo4 interact both in vivo and in vitro, and presumably this physical interaction mediates the inhibition. However, the interaction was demonstrated with anti-TopR1 antibodies that recognize both TopR1 and TopR2, so it is not clear whether one or both reverse gyrases co-immunoprecipitated with PolY/Dpo4. The topoisomerase domain of TopR1 was reported to be responsible for its affinity for PolY/Dpo4. The topoisomerase domains of Sulfolobus TopR1 and TopR2 exhibit a high identity , so it is plausible that TopR2 may exhibit significant affinity for PolY/Dpo4. If this were true, it would imply that TopR2, the only reverse gyrase present and active at 45°C, inhibits PolY/Dpo4. At 80°C, there would be competition between the two reverse gyrases, the results of which would depend on their abundance and their respective affinities for PolY/Dpo4.
Sulfolobus cells at 45°C have no TopR1 although it is required at 80°C. Presumably, TopR1 is inessential at low temperatures and indispensable at high temperature because the mechanical response of DNA is temperature dependent. N. profundicola grows optimally at 45°C and has only one reverse gyrase gene; following exposure to 65°C for two hours, the abundance of the reverse gyrase transcript increases substantially, suggesting that more reverse gyrase is required . This is consistent with the increased frequency of DNA melting at high temperature leading to a greater requirement for reverse gyrase. Possibly, the Sulfolobus TopR1 is the functional homologue of the reverse gyrase of Nautilia, both proteins exhibiting quantitative variations with temperature. T. kodakaraensis also has a single reverse gyrase: a T. kodakaraensis mutant deleted for the corresponding gene is viable at temperatures of 60-90°C but not at temperatures higher than 90°C . Possibly, there are compensatory mutations, or other topoisomerases, present in T. kodakaraensis, compensating for the absence of reverse gyrase but only at temperatures lower than 90°C. In S. islandicus, a strain very closely related to S. solfataricus, no mutant viable at 75°C could be obtained for either TopR1 or TopR2 encoding genes, suggesting that both enzymes are essential . In this work, we found that in S. solfataricus TopR1 is the major reverse gyrase for the control of the topological state DNA. However, the absence of TopR1 can be compensated at least partially by TopR2, its activity being sufficient to control the minor low topological changes at low temperature. At 75°C, TopR2 cannot complement for the absence of TopR1 because DNA melting is much more extensive, and problematic, at this temperature. As TopR2 may have functions not displayed by TopR1, TopR2 mutants may be lethal. These various observations are concordant in implying that TopR1 is required at high temperature and/or for thermoadaptation.
In this report, we demonstrate that S. solfataricus is able to survive at a low temperature (45°C) for a long period, without dividing but with most cells in the culture containing two fully replicated genomes. They are ready to resume a normal cell activity with active cell division as soon as favorable conditions are restored. This property of “cold” resistance may facilitate the spread of S. solfataricus to new niches. We also show that the two reverse gyrases are not regulated in the same way indicating that they do have different and possibly overlapping functions in the cells. We provide here evidence, in addition to our previous results [17, 37], that TopR1 is important for the regulation of the supercoiling density of the genome, which is affected by replication, transcription and recombination, particularly active in dividing cells at high temperature.
We report the first quantification of the numbers of reverse gyrase molecules per cell in S. solfataricus: in actively growing cultures at 80°C, there are approximately 50 molecules of TopR1 and 125 molecules of TopR2 per cell. At 45°C, S. solfataricus does not grow and the reverse gyrase content changes: the amount of TopR1 decreases substantially, although that of TopR2 remains unchanged. These findings in vivo are in agreement with the activities of the two enzymes in vitro. TopR2 exhibits significant positive supercoiling activity at 45°C, a temperature at which TopR1 is not active. TopR1 is inessential at low temperature but required at high temperature and therefore probably involved in thermoadaptation and/or in DNA transaction processes during active division of S. solfataricus cells at 80°C. By contrast, TopR2 is at a constant concentration at both 80°C and 45°C suggesting that TopR2 may be involved in the maintenance of genome stability, particularly in the long term at 45°C when there is no cell division and no replication.
Tris, glycine, SDS, dimethylsulfoxide (DMSO), acrylamide and bis-acrylamide were purchased from Euromedex. Ponceau S, p-coumaric acid, bromophenol blue, brilliant blue R250, MgSO4, Ca(NO3)2, COSO4, CuCl2, ZnSO4, Na2M0O4, Na2B4O7, FeSO4, VOSO4, vitamins, HEPES, hydrogen peroxide, Tween 20, luminol, Triton X-100, sucrose, acetic acid, dithiothreitol (DTT), RNase A, and propidium iodide (PI) were obtained from Sigma. KCl, H2SO4 and KH2PO4 were from Merck. NaCl was from Fischer scientific. Tryptone peptone and yeast extract were obtained from Difco (Becton Dickinson). Ammonium persulfate (APS), N,N,N’,N’-tetra methyl ethylenediamine (TEMED), bovine serum albumin and Bradford reagents were purchased from BioRad. Propan-2-ol, ethanol, sorbitol, HCl, MgCl2, MnCl2 and (NH4)2SO4 were from Prolabo. Glycerol was obtained from Acros organique.
Sulfolobus solfataricus strain P2 (DSMZ 1617) was purchased from the Deutsche Sammlung von Mikroorganismen und Zelkulturen in Braunschweig, Germany.
Reverse gyrase assays
The standard reaction mixture contained 50 mM Tris-HCl pH 8.0, 0.5 mM DTT, 0.5 mM EDTA, 20 mM MgCl2, 100 mM NaCl, 1.25 mM ATP and 0.15 μg of negatively supercoiled pTZ18R DNA. The purified enzymes  were added to the mixture at a molar ratio topoisomerase/DNA of 4, and the mixture was incubated at the indicated temperature for 20 min. The reaction was then stopped by cooling on ice; 0.1% SDS, 25 mg/mL bromophenol blue and sucrose to 15% were added before loading onto the agarose gel.
One-dimensional gel electrophoreses
Electrophoresis was performed in 1.2% agarose gels at room temperature in TEP buffer (36 mM Tris, 30 mM NaH2PO4, 1 mM EDTA, pH 7.8) and run at 3 V/cm for 6 h. Gels were washed in TEP buffer for 15 min, stained with ethidium bromide (2 μg/mL for 30 min) and digitalized under UV light.
Two-dimensional gel electrophoreses
TopR2 activity was analyzed after a two-dimensional gel electrophoresis. The first dimension was run at room temperature in a 1.2% agarose gel in TEP buffer at 3 V/cm for 150 min. The gel was then soaked for 30 min in TEP buffer containing 10 μg/mL chloroquin. The second dimension was run in the same buffer, perpendicularly to the first, at 0.9 V/cm for 14 h. The gel was washed in TEP buffer for 30 min and then stained.
Culture of Sulfolobus solfataricus P2 in liquid medium
Sulfolobus solfataricus P2 was cultured as previously described  at 80°C or 45°C. The cell density was monitored by measuring the optical density at 600 nm (OD600nm) and by flow cytometry. Cells cultivated at 80°C and having reached an exponential growth phase (0.3 < OD600nm < 0.6) were transferred to 45°C and maintained at this low temperature for three weeks either in the same medium, or resuspended every six days with fresh medium pre-heated at 45°C. After three weeks at 45°C, the cultures were transferred back to 80°C.
Phase-contrast and fluorescence microscopy
S. solfataricus cells (1.2 × 109 cells) were collected by centrifugation at room temperature at 5000 × g for 5 min from cultures at 80°C before the shift to 45°C, at 45°C at various times for three weeks, and again when cells were transferred back to 80°C. The cells were washed with medium then centrifuged at 10000 × g for 6 min. Syto 9 and propidium iodide of the LIVE/DEAD Bac Light bacterial viability kit (Molecular Probes) were diluted in the culture medium to a final concentration of 7 μM and 10 μM respectively and used to resuspend cell samples to a final concentration of 107 cells/μL. These samples were incubated for 15 minutes at room temperature in the darkness. The cells were observed in a three-dimensional deconvolution microscope (DMIRE2; Leica) equipped with an HCxPL APO 100 × oil CS objective, NA = 1.40 (Leica). The images were captured on a 10-MHz Cool SNAPHQ 2 CCD camera (Roper Instruments), with a Z-optical spacing of 0.2 μm. METAMORPH software (Universal Imaging Corp.) was used to acquire Z-series, deconvoluted or not, and treat the images. Only one image with sharp fluorescence from the numerous acquired undeconvoluted Z-series is shown for each condition. In all samples, all the cells incorporated one or both stains and at least 200 cells/sample were examined to determine cell size and cell viability. A minimum of three independent measurements were performed for each condition.
Aliquots of S. solfataricus cells were fixed by adding five volumes of 70% ethanol, then diluted with 70% ethanol to obtain a final concentration of 108 cells/mL. The cells were then washed twice with TE buffer (10 mM Tris-HCl pH 7.4 and 10 mM EDTA, pH 8) by centrifugation at 4°C at 10000 x g for 6 min. Samples were kept at 4°C during all steps. The cells were then resuspended in TE buffer containing RNase A (10 μg/mL) to a final concentration of 108 cells/mL. The cell preparations were incubated at 37°C for 120 min and centrifuged at 4°C at 10000 × g for 6 min. The cells were resuspended in the same volume of TE buffer containing propidium iodide (10 μg/mL) and kept at 4°C overnight in the darkness and analyzed with a PAS III Partec Flow cytometer as previously described . Results are shown as representations of combined forward light scatter (FSC) and DNA content distributions.
Crude extract preparation
Cell samples (8.25 × 109 cells) were collected as described for microscopy but were cooled immediately on ice, and then centrifuged at 4°C at 5000 × g for 5 min. The cells were washed twice with buffer containing 20 mM HEPES and 1 M sorbitol then centrifuged at 4°C at 10000 × g for 6 min. The cells were resuspended to a final cell concentration of 4 × 1010 cells/mL in extraction buffer (50 mM Tris-HCl pH 7, 15 mM MgCl2, 50 mM NaCl, 1 mM DTT, 400 mM sorbitol) and were gently disrupted by the addition of 0.5% Triton X-100 and moderate agitation for 15 min at 4°C. Protein concentrations in the resulting crude extracts were determined by the Bradford method with bovine serum albumin as the standard. Aliquots of these protein extracts were denatured with Laemmli buffer and heated at 95°C for 5 min, and resolved by SDS-PAGE.
Detection of reverse gyrases
Primary antibodies were produced by Eurogentec against two epitopes specific for each of the two reverse gyrases of S. solfataricus: PRILYNKQSPTQTEN and EDIQTTMKLLRENIG for anti-TopR1 and GRSKLNIKKYVEDL and YFSEKRKVEEYINNL for anti-TopR2. The choice of these epitopes was based on both the amino acid sequences deduced from the sequences of the genes in S. solfataricus and the published structure of reverse gyrase . These peptides are absent from all other CDS of S. solfataricus. The peptides were synthesized, linked to hemocyanine and used to immunize rabbits. Proteins were separated by SDS-PAGE (10% acrylamide/0.13% bis-acrylamide), then electroblotted onto nitrocellulose membranes (Whatman Protran BA79) over 1 hour at 4°C in transfer buffer (25 mM Tris-HCl, 192 mM glycine, 15% propan-2-ol). Membranes were stained with Ponceau S, washed with TBS -Tween buffer (20 mM Tris-HCl pH 7.6, 13.7 mM NaCl, 0.1% Tween 20) and blocked with 3% milk in TBS-Tween. Membranes were then probed overnight at 4°C with either 1/6000 anti-TopR1 antibodies or 1/4000 anti-TopR2 antibodies. Membranes were then washed with TBS-Tween and probed for 1 hour at 4°C with 1/20000 horseradish-peroxidase-conjugated anti-rabbit IgG from donkey (GE Healthcare). All dilutions of antibodies were in TBS-Tween. Bound antibodies were visualized with ECL mix (100 mM Tris-HCl pH 8.45, 0.009% H2O2, 0.225 mM p-coumaric acid, 1.25 mM luminol). The chemiluminescence and Ponceau S signals were captured on a CCD camera (Image Quant LAS 4000, GE Healthcare) and analyzed with the ImageQuant TL (v 7.0) package.
Estimation of TopR1 and TopR2 number per S. solfataricus cell
To determine the number of copies of the two reverse gyrases in S. solfataricus cells, we established a relation between ECL signal intensity in the crude extracts and the amount of reverse gyrase. Purified recombinant TopR1 and TopR2  were used as standards. Series of quantities from 0.3 to 50 ng were loaded onto gels with the various crude extracts such that the ECL signal intensities can be compared. The ECL signal intensities obtained for various crude extracts for each condition were compared with the TopR1 and TopR2 calibration curves (Additional file 1: Figure S1) and converted into amounts of reverse gyrase. The amount of protein (in ng) was converted into a number of molecules on the basis of the theoretical molecular weight of the corresponding reverse gyrase. Only membranes for which the Ponceau S staining of the various crude extracts loaded was homogeneous were used for these analyses. Each experiment was performed at least three times independently, and the results reported are the mean values.
This work was supported by CNRS, EDF (RB-2003-16) and IFR 115.
We thank Magali Prigent for her help with fluorescent microscopy and Jean-Luc Ferat for his help with flow cytometer experiments. We thank Hélène Débat, Florence Constantinesco, Christiane Elie, Adrienne Kish and Martine Mathieu for helpful discussions. We are indebted to Patrick Forterre for support during this work.
- Liu LFL, Wang JCJ: Supercoiling of the DNA template during transcription. Proc Natl Acad Sci U S A. 1987, 84: 7024-7027. 10.1073/pnas.84.20.7024View ArticlePubMedPubMed CentralGoogle Scholar
- Champoux JJ: DNA topoisomerases: structure, function, and mechanism. Annu Rev Biochem. 2001, 70: 369-413. 10.1146/annurev.biochem.70.1.369View ArticlePubMedGoogle Scholar
- Chen SH, Chan N-L, Hsieh T-S: New Mechanistic and Functional Insights into DNA Topoisomerases. Annu Rev Biochem. 2013, 82: 139-170. 10.1146/annurev-biochem-061809-100002View ArticlePubMedGoogle Scholar
- Mirambeau G, Duguet M, Forterre P: Atp-Dependent Dna Topoisomerase From the Archaebacterium Sulfolobus-Acidocaldarius - Relaxation of Supercoiled Dna at High-Temperature. J Mol Biol. 1984, 179: 559-563. 10.1016/0022-2836(84)90080-9View ArticlePubMedGoogle Scholar
- Kikuchi A, Asai K: Reverse gyrase–a topoisomerase which introduces positive superhelical turns into DNA. Nature. 1984, 309: 677-681. 10.1038/309677a0View ArticlePubMedGoogle Scholar
- Forterre P, Mirambeau G, Jaxel C, Nadal M, Duguet M: High positive supercoiling in vitro catalyzed by an ATP and polyethylene glycol-stimulated topoisomerase from Sulfolobus acidocaldarius. EMBO J. 1985, 4: 2123-2128.PubMedPubMed CentralGoogle Scholar
- Nakasu S, Kikuchi A: Reverse gyrase; ATP-dependent type I topoisomerase from Sulfolobus. EMBO J. 1985, 4: 2705-2710.PubMedPubMed CentralGoogle Scholar
- Nadal M, Jaxel C, Portemer C, Forterre P, Mirambeau G, Duguet M: Reverse gyrase of Sulfolobus: purification to homogeneity and characterization. Biochemistry. 1988, 27: 9102-9108. 10.1021/bi00426a006View ArticlePubMedGoogle Scholar
- Nadal M: Reverse gyrase: an insight into the role of DNA-topoisomerases. Biochimie. 2007, 89: 447-455. 10.1016/j.biochi.2006.12.010View ArticlePubMedGoogle Scholar
- Bouthier de la Tour C, Portemer C, Nadal M, Stetter KO, Forterre P, Duguet M: Reverse gyrase, a hallmark of the hyperthermophilic archaebacteria. J Bacteriol. 1990, 172: 6803-6808.PubMedPubMed CentralGoogle Scholar
- Bouthier de la Tour C, Portemer C, Huber R, Forterre P, Duguet M: Reverse gyrase in thermophilic eubacteria. J Bacteriol. 1991, 173: 3921-3923.PubMedPubMed CentralGoogle Scholar
- Forterre P: A hot story from comparative genomics: reverse gyrase is the only hyperthermophile-specific protein. Trends Genet. 2002, 18: 236-237. 10.1016/S0168-9525(02)02650-1View ArticlePubMedGoogle Scholar
- Brochier-Armanet C, Forterre P: Widespread distribution of archaeal reverse gyrase in thermophilic bacteria suggests a complex history of vertical inheritance and lateral gene transfers. Archaea. 2007, 2: 83-93.View ArticlePubMedPubMed CentralGoogle Scholar
- Nadal M, Mirambeau G, Forterre P, Reiter WD, Duguet M: Positively Supercoiled Dna in a Virus-Like Particle of an Archaebacterium. Nature. 1986, 321: 256-258. 10.1038/321256a0. 10.1038/321256a0View ArticleGoogle Scholar
- Lim HM, Lee HJ, Jaxel C, Nadal M: Hin-mediated inversion on positively supercoiled DNA. J Biol Chem. 1997, 272: 18434-18439. 10.1074/jbc.272.29.18434View ArticlePubMedGoogle Scholar
- Bell SD, Jaxel C, Nadal M, Kosa PF, Jackson SP: Temperature, template topology, and factor requirements of archaeal transcription. Proc Natl Acad Sci U S A. 1998, 95: 15218-15222. 10.1073/pnas.95.26.15218View ArticlePubMedPubMed CentralGoogle Scholar
- Garnier F, Nadal M: Transcriptional analysis of the two reverse gyrase encoding genes of Sulfolobus solfataricus P2 in relation to the growth phases and temperature conditions. Extremophiles. 2008, 12: 799-809. 10.1007/s00792-008-0186-2View ArticlePubMedGoogle Scholar
- Menzel R, Gellert M: Regulation of the genes for E. coli DNA gyrase: homeostatic control of DNA supercoiling. Cell. 1983, 34: 105-113. 10.1016/0092-8674(83)90140-XView ArticlePubMedGoogle Scholar
- Peter BJ, Arsuaga J, Breier AM, Khodursky AB, Brown PO, Cozzarelli NR: Genomic transcriptional response to loss of chromosomal supercoiling in Escherichia coli. Genome Biol. 2004, 5: R87- 10.1186/gb-2004-5-11-r87View ArticlePubMedPubMed CentralGoogle Scholar
- Kampmann M, Stock D: Reverse gyrase has heat-protective DNA chaperone activity independent of supercoiling. Nucleic Acids Res. 2004, 32: 3537-3545. 10.1093/nar/gkh683View ArticlePubMedPubMed CentralGoogle Scholar
- Confalonieri F, Elie C, Nadal M, Bouthier de La Tour C, Forterre P, Duguet M: Reverse Gyrase: A Helicase-Like Domain and a Type 1 Topoisomerase in the Same Polypeptide. Volume. 1993, 90: 4753-4757.Google Scholar
- Jaxel C, Bouthier de la Tour C, Duguet M, Nadal M: Reverse gyrase gene from Sulfolobus shibatae B12: gene structure, transcription unit and comparative sequence analysis of the two domains. Nucleic Acids Res. 1996, 24: 4668-4675. 10.1093/nar/24.23.4668View ArticlePubMedPubMed CentralGoogle Scholar
- Gangloff S, McDonald JP, Bendixen C, Arthur L, Rothstein R: The yeast type I topoisomerase Top3 interacts with Sgs1, a DNA helicase homolog: a potential eukaryotic reverse gyrase. Mol Cell Biol. 1994, 14: 8391-8398.View ArticlePubMedPubMed CentralGoogle Scholar
- Harmon FG, DiGate RJ, Kowalczykowski SC: RecQ helicase and topoisomerase III comprise a novel DNA strand passage function: a conserved mechanism for control of DNA recombination. Mol Cell. 1999, 3: 611-620. 10.1016/S1097-2765(00)80354-8View ArticlePubMedGoogle Scholar
- Mankouri HW, Hickson ID: The RecQ helicase-topoisomerase III-Rmi1 complex: a DNA structure-specific ‘dissolvasome’?. Trends Biochem Sci. 2007, 32: 538-546. 10.1016/j.tibs.2007.09.009View ArticlePubMedGoogle Scholar
- Perugino G, Valenti A, D’amaro A, Rossi M, Ciaramella M: Reverse gyrase and genome stability in hyperthermophilic organisms. Biochem Soc Trans. 2009, 37: 69-73. 10.1042/BST0370069View ArticlePubMedGoogle Scholar
- Slesarev AI, Kozyavkin SA: DNA substrate specificity of reverse gyrase from extremely thermophilic archaebacteria. J Biomol Struct Dyn. 1990, 7: 935-942. 10.1080/07391102.1990.10508533View ArticlePubMedGoogle Scholar
- Hsieh T-S, Plank JL: Reverse gyrase functions as a DNA renaturase: annealing of complementary single-stranded circles and positive supercoiling of a bubble substrate. J Biol Chem. 2006, 281: 5640-5647.View ArticlePubMedGoogle Scholar
- Napoli A, Valenti A, Salerno V, Nadal M, Garnier F, Rossi M, Ciaramella M: Reverse gyrase recruitment to DNA after UV light irradiation in Sulfolobus solfataricus. J Biol Chem. 2004, 279: 33192-33198. 10.1074/jbc.M402619200View ArticlePubMedGoogle Scholar
- Napoli AA, Valenti AA, Salerno VV, Nadal MM, Garnier FF, Rossi MM, Ciaramella MM: Functional interaction of reverse gyrase with single-strand binding protein of the archaeon Sulfolobus. Nucleic Acids Res. 2004, 33: 564-576.View ArticleGoogle Scholar
- Wadsworth RIR, White MFM: Identification and properties of the crenarchaeal single-stranded DNA binding protein from Sulfolobus solfataricus. Nucleic Acids Res. 2001, 29: 914-920. 10.1093/nar/29.4.914View ArticlePubMedPubMed CentralGoogle Scholar
- Valenti A, Perugino G, Nohmi T, Rossi M, Ciaramella M: Inhibition of translesion DNA polymerase by archaeal reverse gyrase. Nucleic Acids Res. 2009, 37: 4287-4295. 10.1093/nar/gkp386View ArticlePubMedPubMed CentralGoogle Scholar
- Valenti A, Perugino G, Varriale A, D'Auria S, Rossi M, Ciaramella M: The archaeal topoisomerase reverse gyrase is a helix-destabilizing protein that unwinds four-way DNA junctions. J Biol Chem. 2010, 285: 36532-36541. 10.1074/jbc.M110.169029View ArticlePubMedPubMed CentralGoogle Scholar
- Campbell BJ, Smith JL, Hanson TE, Klotz MG, Stein LY, Lee CK, Wu D, Robinson JM, Khouri HM, Eisen JA, Cary SC: Adaptations to submarine hydrothermal environments exemplified by the genome of Nautilia profundicola. PLoS Genet. 2009, 5: e1000362- 10.1371/journal.pgen.1000362View ArticlePubMedPubMed CentralGoogle Scholar
- Atomi H, Matsumi R, Imanaka T: Reverse gyrase is not a prerequisite for hyperthermophilic life. J Bacteriol. 2004, 186: 4829-4833. 10.1128/JB.186.14.4829-4833.2004View ArticlePubMedPubMed CentralGoogle Scholar
- Zhang C, Tian B, Li S, Ao X, Dalgaard K, Gökce S, Liang Y, She Q: Genetic manipulation in Sulfolobus islandicus and functional analysis of DNA repair genes. Biochem Soc Trans. 2013, 41: 405-410. 10.1042/BST20120285View ArticlePubMedGoogle Scholar
- Bizard A, Garnier F, Nadal M: TopR2, the Second Reverse Gyrase of Sulfolobus solfataricus, Exhibits Unusual Properties. J Mol Biol. 2011, 408: 839-849. 10.1016/j.jmb.2011.03.030View ArticlePubMedGoogle Scholar
- Lübben M, Schäfer G: Chemiosmotic energy conversion of the archaebacterial thermoacidophile Sulfolobus acidocaldarius: oxidative phosphorylation and the presence of an F0-related N, N'-dicyclohexylcarbodiimide-binding proteolipid. J Bacteriol. 1989, 171: 6106-6116.PubMedPubMed CentralGoogle Scholar
- Lin H-KH, Chase SFS, Laue TMT, Jen-Jacobson LL, Trakselis MAM: Differential temperature-dependent multimeric assemblies of replication and repair polymerases on DNA increase processivity. Biochemistry. 2012, 51: 7367-7382. 10.1021/bi300956tView ArticlePubMedPubMed CentralGoogle Scholar
- Choi J-Y, Eoff RL, Pence MG, Wang J, Martin MV, Kim E-J, Folkmann LM, Guengerich FP: Roles of the four DNA polymerases of the crenarchaeon Sulfolobus solfataricus and accessory proteins in DNA replication. J Biol Chem. 2011, 286: 31180-31193. 10.1074/jbc.M111.258038View ArticlePubMedPubMed CentralGoogle Scholar
- Hjort K, Bernander R: Changes in Cell Size and DNA Content in Sulfolobus Cultures during Dilution and Temperature Shift Experiments. J Bacteriol. 1999, 181: 5669-5675.PubMedPubMed CentralGoogle Scholar
- Boulos L, Prévost M, Barbeau B, Coallier J, Desjardins R: LIVE/DEAD® BacLight™: application of a new rapid staining method for direct enumeration of viable and total bacteria in drinking water. J Microbiol Methods. 1999, 37: 77-86. 10.1016/S0167-7012(99)00048-2View ArticlePubMedGoogle Scholar
- Leuko S, Legat A, Fendrihan S, Stan-Lotter H: Evaluation of the LIVE/DEAD BacLight kit for detection of extremophilic archaea and visualization of microorganisms in environmental hypersaline samples. Appl Environ Microbiol. 2004, 70: 6884-6886. 10.1128/AEM.70.11.6884-6886.2004View ArticlePubMedPubMed CentralGoogle Scholar
- De Rosa M, Gambacorta A, Nicolaus B, Sodano S, Bu'lock JD: Structural regularities in tetraether lipids of Caldariella and their biosynthetic and phyletic implications. Phytochemistry. 1980, 19: 833-836. 10.1016/0031-9422(80)85121-1. 10.1016/0031-9422(80)85121-1View ArticleGoogle Scholar
- Bernander RR, Poplawski AA: Cell cycle characteristics of thermophilic archaea. J Bacteriol. 1997, 179: 4963-4969.PubMedPubMed CentralGoogle Scholar
- Esser D, Pham TK, Reimann J, Albers S-V, Siebers B, Wright PC: Change of Carbon Source Causes Dramatic Effects in the Phospho-Proteome of the Archaeon Sulfolobus solfataricus. J Proteome Res. 2012, 11: 4823-4833. 10.1021/pr300190kView ArticlePubMedGoogle Scholar
- Barry RC, Young MJ, Stedman KM, Dratz EA: Proteomic mapping of the hyperthermophilic and acidophilic archaeon Sulfolobus solfataricus P2. Electrophoresis. 2006, 27: 2970-2983. 10.1002/elps.200500851View ArticlePubMedGoogle Scholar
- Reimann J, Esser D, Orell A, Amman F, Pham TK, Noirel J, Lindås A-C, Bernander R, Wright PC, Siebers B, Albers S-V: Archaeal Signal Transduction: Impact of Protein Phosphatase Deletions on Cell Size, Motility, and Energy Metabolism in Sulfolobus acidocaldarius. Mol Cell Proteomics. 2013, 12: 3908-3923. 10.1074/mcp.M113.027375View ArticlePubMedPubMed CentralGoogle Scholar
- Perugino G, Vettone A, Illiano G, Valenti A, Ferrara MC, Rossi M, Ciaramella M: Activity and regulation of an archaeal DNA-alkyltransferase: conserved protein involved in repair of DNA alkylation damage. J Biol Chem. 2012, 287: 4222-4231. 10.1074/jbc.M111.308320View ArticlePubMedPubMed CentralGoogle Scholar
- Napoli A, Zivanovic Y, Bocs C, Buhler C, Rossi M, Forterre P, Ciaramella M: DNA bending, compaction and negative supercoiling by the architectural protein Sso7d of Sulfolobus solfataricus. Nucleic Acids Res. 2002, 30: 2656-2662. 10.1093/nar/gkf377View ArticlePubMedPubMed CentralGoogle Scholar
- Lundgren M, Bernander R: Genome-wide transcription map of an archaeal cell cycle. Proc Natl Acad Sci U S A. 2007, 104: 2939-2944. 10.1073/pnas.0611333104View ArticlePubMedPubMed CentralGoogle Scholar
- Saifi B, Ferat J-L, Marinus MG: Replication Fork Reactivation in a dnaC2 Mutant at Non-Permissive Temperature in Escherichia coli. PLoS One. 2012, 7: 3613-View ArticleGoogle Scholar
- She QQ, Singh RKR, Confalonieri FF, Zivanovic YY, Allard GG, Awayez MJM, Chan-Weiher CCC, Clausen IGI, Curtis BAB, De Moors AA, Erauso GG, Fletcher CC, Gordon PMP, Jong IIH-D, Jeffries ACA, Kozera CJC, Medina NN, Peng XX, Thi-Ngoc HPH, Redder PP, Schenk MEM, Theriault CC, Tolstrup NN, Charlebois RLR, Doolittle WFW, Duguet MM, Gaasterland TT, Garrett RAR, Ragan MAM, Sensen CWC, et al: The complete genome of the crenarchaeon Sulfolobus solfataricus P2. Proc Natl Acad Sci U S A. 2001, 98: 7835-7840. 10.1073/pnas.141222098View ArticlePubMedPubMed CentralGoogle Scholar
- Rodríguez AC, Stock D: Crystal structure of reverse gyrase: insights into the positive supercoiling of DNA. EMBO J. 2002, 21: 418-426. 10.1093/emboj/21.3.418View ArticlePubMedPubMed CentralGoogle Scholar
This article is published under license to BioMed Central Ltd. This is an Open Access article distributed under the terms of the Creative Commons Attribution License (http://creativecommons.org/licenses/by/4.0), which permits unrestricted use, distribution, and reproduction in any medium, provided the original work is properly credited. The Creative Commons Public Domain Dedication waiver (http://creativecommons.org/publicdomain/zero/1.0/) applies to the data made available in this article, unless otherwise stated.