Figure 1From: Insight into the cellular involvement of the two reverse gyrases from the hyperthermophilic archaeon Sulfolobus solfataricusEffect of low temperature on the activities of TopR1 and TopR2. Standard positive supercoiling reactions were performed in the presence of a limiting amount of TopR1 (A) or TopR2 (B and C). The reaction products were analyzed by one-dimensional gel electrophoresis (A and B) and for TopR2 also by two-dimensional gel electrophoresis (C). In panels A, B and C, the incubation temperature is indicated above the gels. Pictures in panel C below the 2D gels schematize the position of the various forms of DNA in 2D gel electrophoresis. SC + and SC ++ indicate the positions of positively supercoiled and highly positively supercoiled DNA, respectively, Rel the position of the relaxed DNA, oc the position of nicked DNA, and SC- the position of negatively supercoiled DNA substrate.Back to article page