- Research article
- Open Access
N-terminal region of Saccharomyces cerevisiae eRF3 is essential for the functioning of the eRF1/eRF3 complex beyond translation termination
© Urakov et al; licensee BioMed Central Ltd. 2006
Received: 27 April 2006
Accepted: 11 October 2006
Published: 11 October 2006
Termination of translation in eukaryotes requires two release factors, eRF1, which recognizes all three nonsense codons and facilitates release of the nascent polypeptide chain, and eRF3 stimulating translation termination in a GTP-depended manner. eRF3 from different organisms possess a highly conservative C region (eRF3C), which is responsible for the function in translation termination, and almost always contain the N-terminal extension, which is inessential and vary both in structure and length. In the yeast Saccharomyces cerevisiae the N-terminal region of eRF3 is responsible for conversion of this protein into the aggregated and functionally inactive prion form.
Here, we examined functional importance of the N-terminal region of a non-prion form of yeast eRF3. The screen for mutations which are lethal in combination with the SUP35-C allele encoding eRF3C revealed the sup45 mutations which alter the N-terminal domain of eRF1 and increase nonsense codon readthrough. However, further analysis showed that synthetic lethality was not caused by the increased levels of nonsense codon readthrough. Dominant mutations in SUP35-C were obtained and characterized, which remove its synthetic lethality with the identified sup45 mutations, thus indicating that synthetic lethality was not due to a disruption of interaction with proteins that bind to this eRF3 region.
These and other data demonstrate that the N-terminal region of eRF3 is involved both in modulation of the efficiency of translation termination and functioning of the eRF1/eRF3 complex outside of translation termination.
Termination of protein synthesis takes place in ribosomes in a response to stop codons in the "decoding" site. The proteins that control this process are called polypeptide chain release factors (RFs). In eubacteria there are two class 1 polypeptide chain release factors, RF1 and RF2, recognizing UAA/UAG and UAA/UGA stop codons, respectively, whereas eukaryotes employ only one such factor, eRF1, which is able to decode all three nonsense codons . In addition, translation termination in eubacteria and eukaryotes is stimulated by class 2 release factors, RF3 and eRF3, respectively. Both class 2 factors are GTPases enhancing the termination efficiency by stimulating activity of class 1 release factors in a GTP-dependent manner [2–4]. RF3 is able to remove RF1 or RF2 from the ribosome after hydrolysis of the ester bond in peptidyl tRNA, i.e. to stimulate recycling of class 1 factors . In contrast to eubacterial RF3, eukaryotic eRF3 functions in termination by applying its GTPase activity to assist eRF1 with the stop codon recognition and/or ensures rapid and efficient hydrolysis of peptidyl tRNA . Importantly, this was shown upon the excess of eRF1 when quantitative peptide release did not require recycling of eRF1. In the yeast Saccharomyces cerevisiae eRF1 and eRF3 are encoded by the essential SUP45 and SUP35 genes, and often designated as Sup45p and Sup35p, respectively .
Members of the eRF1 family have been identified in a number of eukaryotes and they all show a high degree of sequence similarity. Determination of the crystal structure of human eRF1 has shown that it is composed of three domains and resembles by overall shape and dimensions a tRNA molecule with the N-terminal and middle domains corresponding to the tRNA's anticodon stem and aminoacyl acceptor stem, respectively . eRF3 also has a complex structure and can be divided into at least two regions: a non-conserved N-terminal region and a conserved C-terminal region, showing a considerable sequence similarity to the translation elongation factor eEF1A, which brings aminoacyl-tRNAs to the ribosomal A site. The C-terminal part (domain C) of the S. cerevisiae eRF3 is responsible for the function of the protein in translation termination. The N-terminal region of yeast eRF3 is non-essential for the translation termination and viability and may be further subdivided into the N-terminal (N) domain, which was shown to be required for maintenance of the cytoplasmically-inherited prion determinant [PSI+] [8, 9] and the middle (M) domain, which also is important for [PSI+] propagation [10, 11]. The nonsense suppressor phenotype of [PSI+] reflects eRF3 aggregation and functional inactivation [12, 13]. Thus, the N domain of eRF3 may inhibit translation termination in S. cerevisiae in a [PSI+] prion-dependent manner. However, other data have shown that this domain also affects function of a non-prion form of yeast eRF3, since deletion of the eRF3 N-terminal extension (N and M domains) decreased nonsense codon readthrough caused by depletion of eRF1 . This agrees with the results showing that N-terminal extension of the Schizosaccharomyces pombe eRF3 may inhibit translation termination by blocking eRF1 binding to eRF3 . However, the N-terminal region of eRF3 from the filamentous fungus Podospora anserina confers quite opposite effect on translation termination, since deletion of this protein region caused suppression of nonsense mutations . The effects of N-terminal truncation of eRF3 have a species-specific character probably because of the lack of sequence similarity between the N-terminal regions of eRF3 homologs from different organisms. Nevertheless, despite of structural dissimilarity, the N-terminal regions of eRF3 from yeast and mammals are able to interact with poly(A)+ binding protein PABP, which probably serves for coupling termination and initiation steps of translation [17, 18] and for regulation of mRNA deadenylation and decay . The N-terminal region of S. cerevisiae eRF3 also is able to bind other proteins, such as Itt1p, a protein with unknown function, and Sla1p assisting in the cytoskeleton assembly [20, 21]. These interactions may modulate translation termination efficiency and link translation with other cellular processes.
Biochemical studies of S. cerevisiae eRF3 are hampered by its propensity to aggregate [22, 23]. This makes genetic approach preferable to investigate function of this release factor. Here, we identified mutations which alter eRF1 and make the N-terminal region of eRF3 indispensable. The study of these mutations and their lethal interaction with the SUP35 deletion allele, encoding the eRF3 C-terminal domain, indicate the role of N-terminal region of this factor in non-translational function of the eRF1/eRF3 termination complex in yeast.
Identification and characterization of mutants in which deletion of the eRF3 NM region is lethal
The SUP35-C strain YJV159-rS35C carrying the pHT-SUP35 (SUP35 ADE3) centromeric plasmid was mutagenized with UV irradiation and over 150,000 of grown colonies were screened using a yeast colony color sectoring assay. Among them, 23 were of Sect- phenotype. Such clones could arise due to mutations in the SUP35-C gene, since it is essential , or due to mutations in other genes. To discriminate between these possibilities, the centromeric pRS315-SUP35C (SUP35-C LEU2) plasmid was introduced into the YJV159-rS35C strain carrying pHT-SUP35. In 22 cases this restored Sect+ phenotype indicating that mutations were within SUP35-C and corresponding mutants were discarded. One mutant, which segregated white sectors very poorly, preserved its leaky Sect- phenotype in the presence of the pRS315-SUP35C plasmid. The mutant, designated as YJV-rS35C-SL23, could not grow at 37°C on YPD plate. This mutant carrying pHT-SUP35 was transformed with a genomic library based on the LEU2 centromeric plasmid p366, and two transformants able to grow at non-permissive temperature were selected. The obtained temperature resistant transformants were characterized by the frequent loss of pHT-SUP35 at permissive temperature, thus being Sect+. Plasmid from one transformant was isolated and partial sequencing of the cloned DNA fragment has shown that it contained the SUP45 gene. Subsequent delimitation of the functional region of the isolated DNA fragment indicated that SUP45 was responsible for both temperature resistance and Sect+ phenotype. Transformation of this mutant with various SUP45-carrying plasmids caused the same effects. Thus, mutation that made the NM region of eRF3 indispensable arose in the SUP45 gene. Transformation of the mutant YJV-rS35C-SL23 carrying pHT-SUP35 with the multicopy plasmid YEplac181-SUP35NM encoding the eRF3 NM region did not restore the Sect+ phenotype. This indicated that expression of the entire eRF3 protein is necessary for viability of this mutant.
Synthetic lethality was also reproduced in the S1-R-H8 strain. This strain, carrying SUP35-C, was disrupted for SUP45 and contained the URA3 SUP45 centromeric plasmid pRG415-SUP45, which could not be replaced with the plasmid pRS315-sup45-sl23 carrying the mutant sup45-sl23 ts allele. In contrast, in the control strain S1-H8 with chromosomal wild type SUP35, pRS315-sup45-sl23 could replace the resident pRG415-SUP45 plasmid. The lethal effect of combination with SUP35-C was also found for another sup45 mutation, sup45-36 ts : the pRS315-sup45-36 plasmid did not replace pRG415-SUP45 in the S1-R-H8 strain, but replaced this plasmid in the SUP45 disruptant S1-H8 bearing the wild type SUP35 gene.
The possible reason of lethality manifested by the sup45 mutations in combination with SUP35-C is the decreased level of eRF1. However, the amount of eRF1 was not affected in the sup45-36 ts  and sup45-sl23 ts mutants (data not shown). S. cerevisiae eRF1 and eRF3 interact with each other through the binding sites located at their C-termini [26, 27], though the eRF3 N-terminal region also may be involved in this interaction . Mutations in eRF1 breaking its interaction with eRF3 decrease cell viability . The observed disparity in genetic interaction of the sup45-sl23 ts and sup45-36 ts mutations with SUP35 and SUP35-C suggests that these mutations influence binding of eRF1 to eRF3, and this effect is more pronounced when eRF3 lacks its N-terminal region. However, the study of the ability of Escherichia coli-expressed His6-eRF1 and His6-eRF1-sl23 immobilized on TALON beads to precipitate eRF3 and eRF3C from yeast cell lysates  did not show a noticeable effect of sup45-sl23 ts mutation on the interaction of eRF1 with both eRF3 and eRF3C (data not shown).
Mutations in SUP35-C abolishing its synthetic lethality with sup45-sl23ts or sup45-36ts
Mutations in SUP35-C which make it compatible with sup45-sl23 ts were obtained by passing the corresponding plasmid in the E. coli "mutator" strain. Five independent pools of the mutagenized plasmid were obtained and used to transform the strain YJV-rS35C-SL23 which carried the chromosomal sup45-sl23 ts and SUP35-C alleles and the centromeric SUP35 ADE3 pHT-SUP35 plasmid. Over 100,000 of transformed cells were incubated on leucine omission medium at 37°C and 116 Leu+ temperature resistant clones were selected. Streaking of these transformants on leucine omission medium showed that 75 of them produced colonies with Sect+ phenotype. The plasmid DNA from 8 Sect+ transformants was isolated and used for repeated transformation of the strain YJV-rS35C-SL23 carrying pHT-SUP35. All Leu+ transformants bearing the isolated plasmids were temperature resistant and able to lose the resident pHT-SUP35 (SUP35 ADE3 URA3) plasmid on medium supplemented with uracil, suggesting that they carried mutant SUP35-C. It is noteworthy that, since these mutations were dominant, they restored the normal growth rate and temperature resistance of the sup45-sl23 ts mutant YJV-rS35C-SL23 carrying the chromosomal SUP35-C allele. These SUP35-C mutant alleles supported viability of the sup45-sl23 ts mutant 33G-D373-rSL23-ΔS35 disrupted for SUP35 and allowed its growth at non-permissive temperature, indicating that suppression of temperature sensitivity did not depend on genetic background.
Sequencing of the mutant SUP35-C alleles revealed that they had nucleotide substitutions that caused either Glu-539 to Lys (five cases, isolated from four mutagenized plasmid pools), Ala-596 to Val (two cases, both derived from the same plasmid pool) or Ala-597 to Val (one case) replacements. It is noteworthy that these amino acid substitutions occurred in the positions located downstream to the eRF3 regions implicated in GTP hydrolysis [3, 8]. The corresponding alleles were respectively designated as SUP35-C14, SUP35-C11 and SUP35-C42.
Synthetic lethality between sup45-sl23ts or sup45-36ts and SUP35-C is not caused by the increased levels of nonsense codon readthrough
To confirm this we have constructed the strains 33G-D373-rSL23-r35C and 33G-D373-r36-r35C, which carried, respectively, the sup45-sl23 ts and sup45-36 ts mutations and the SUP35-C allele, as well as the centromeric pCM183-SUP45 plasmid with the TRP1 selectable marker and SUP45 under the control of regulatable tetO2 promoter. The synthetic lethality of sup45 ts and SUP35-C combination in these strains was manifested only upon the repression of wild type SUP45 by doxycycline. As a control we used the isogenic strains 33G-D373-rSL23 and 33G-D373-r36, which had the wild type SUP35 gene instead of the SUP35-C allele. To monitor the levels of nonsense codon readthrough upon the repression of wild type SUP45, all strains were transformed with either one of the pUKC815, 817, 818, 819 plasmids.
Here, we have demonstrated that the sup45-sl23 ts mutation did not decrease the levels of eRF1, and sup45-sl23 ts extra copies neither suppressed synthetic lethality, nor decreased nonsense codon readthrough. This indicates that both defect of translation termination and lethal interaction between sup45-sl23 ts and SUP35-C were not due to a shortage of eRF1, making improbable suggestion that these effects were due to inefficient recycling of mutant eRF1 by eRF3 lacking the N-terminal region. Furthermore, such suggestion contradicts to our previous data indicating that the NM region of eRF3 may negatively affect its ability to recycle eRF1. It was shown that shortage of eRF1 in the strain expressing full length eRF3 resulted in higher levels of nonsense codon readthrough than in the strain expressing eRF3C , while deletion of the eRF3 NM region did not affect nonsense readthrough at the normal levels of eRF1 [examined in the presence of the tRNA suppressor SUP16 (SUQ5) to enable detection of a decrease in nonsense readthrough levels; our unpublished data]. This suggests that the presence of eRF3 NM region has a significant negative effect on translation termination only at the decreased eRF1 levels. Deficiency in eRF1 should make its recycling important for translation termination and, therefore, these data agreed with the hypothesis that eRF3 is involved in the recycling of eRF1 and indicated that NM region of eRF3 interferes with this process. Thus, deletion of NM region should enhance the eRF3 activity, making improbable that the reason of synthetic lethal interaction between SUP35-C and sup45-sl23 ts or sup45-36 ts lies in inefficient translation termination. Furthermore, the results of this work suggest that combination of SUP35-C with either sup45-sl23 ts or sup45-36 ts is lethal not due to an increase of nonsense codon readthrough.
The obtained SUP35-C mutations compensate for the lack of the eRF3 N-terminal region, thus ruling out the possibility that synthetic lethality was due to a disruption of interaction with proteins that bind to this eRF3 region, including interaction with the poly(A)+-binding protein PABP [18, 19]. This makes unlikely that synthetic lethality was caused by a disturbance in regulation of mRNA decay or by alteration of translation initiation by its uncoupling from termination. The latter is also supported by the observation that synthetic lethality was not accompanied by noticeable alteration of the polyribosomal profile.
Both identified sup45 mutations, sup45-sl23 ts and sup45-36 ts , that make the N-terminal region of eRF3 indispensable, altered the N domain of eRF1 and, furthermore, they caused substitutions of amino acids located in proximity to each other. Both sup45-sl23 ts and sup45-36 ts increased the levels of nonsense codon readthrough and, therefore, affected translation termination. In addition, they did not allow yeast cells to grow at 37°C. Earlier it was found that temperature sensitivity of the sup45-36 ts mutant was not due to a defect of translation termination. Inability to grow at non-permissive temperature was caused by the impairment of cytokinesis resulting from mislocalization of the eRF1-binding protein, myosin light chain Mlc1p . Though cytokinesis defect was not shown for the sup45-sl23 ts mutant, its temperature sensitivity also was not caused by a defect in translation termination, suggesting that yeast eRF1 may have different functions outside of translation. It is also important that the compensatory SUP35-C mutations alleviated both effects of the sup45-sl23 ts mutation, temperature sensitivity and increased nonsense readthrough, indicating that interaction with eRF3 probably is important for eRF1 not only for promoting translation termination, but also for its uncharacterized function. In contrast, interaction with eRF3 is not required for function of eRF1 in cytokinesis, since eRF1 in a complex with Mlc1p can not bind eRF3 .
The data presented here show that the effect of synthetic lethality can not be deduced from a defect of translation termination. The study of the SUP35-C compensatory mutations has shown that they are able to suppress both temperature sensitivity of sup45-sl23 ts and its lethal interaction with SUP35-C. However, temperature sensitivity of sup45-sl23 ts and its synthetic lethality with SUP35-C are caused by different reasons, since increasing the copy-number of sup45-sl23 ts alleviated temperature sensitivity but did not abolish synthetic lethality. Therefore, these data show that the eRF1/eRF3 complex may be involved in different processes outside of translation termination.
In many yeast species the N-terminal part of the eRF3 release factors is responsible for their prion properties [29–33]. Such conservation suggests that the prion properties of yeast eRF3 have biological significance. In this work we have shown that the prion-forming domain of eRF3 from the yeast S. cerevisiae is important for its functioning outside of translation termination. However, this does not mean that the N-terminal extension of eRF3 factors from other organisms plays a similar role. Indeed, point mutations altering S. cerevisiae eRF3C can compensate for the lack of its NM region indicating that the C domain of eRF3 from other organisms may contain all information required for this function.
Media and strains
Yeast strains were grown on standard organic (YPD) and synthetic (SD) media . The modified YPD media (YPDm) [2% peptone, 0.5% yeast extract (Oxoid), 3% glucose and 2% agar] was used in colony color sectoring assay experiments, because it favored accumulation of red pigment in the ade2 mutants. The 5-fluoroorotic acid (5FOA) medium was prepared as described . The expression of the tetO2-controlled SUP45 was repressed by incubation of corresponding strains on medium which contained 10 μg/ml doxycycline and was selective for the pCM183-SUP45 plasmid. LB and 2 × YT media were used for bacteria . Appropriate amounts of antibiotics, amino acids, and bases were added when necessary. Yeast cells were grown at 30°C, if not indicated otherwise, and bacteria at 37°C. DNA transformation of lithium acetate-treated yeast cells was performed as described previously . E. coli cells were transformed by the method described in .
The E. coli mutator strain XL1-Red [endA 1 gyrA 96 thi- 1 hsdR 17 supE 44 relA 1 lac mutD 5 mutS mutT Tn10 (Tetr)] (Stratagene) was used for obtaining mutations in the plasmid SUP35-C gene. For this, the plasmid pRS315-SUP35C was introduced into this E. coli strain. After incubation for 48 h, over 500 transformant colonies were suspended in 10 ml of 2 × YT supplemented with ampicillin (100 μg ml-1). Cell culture was additionally incubated for 8 h with shaking, plasmid DNA was isolated and used to transform yeast. The E. coli strain DH5α [supE 44 Dlac U169 (f80 lacZ DM15) hsdR 17 recA 1 endA 1 gyrA 96 thi-1 relA 1]  was used in cloning experiments.
S cerevisiae strains used in this study
MAT a ade2 ade3 his3 leu2-3,112 trp1 ura3
Same as in YJV159, but with SUP35-C instead of SUP35
Same as in YJV159-rS35C, but with sup45-sl23 ts instead of SUP45
MAT a ura3-52 leu2-3 112 trp1-289 his7-1 lys9-A21 pheA10 ade2-144, 717
Same as in 33G-D373, but with sup45-sl23 ts instead of SUP45
Same as 33G-D373-rSL23 but with SUP35-C instead of SUP35
Same as in 33G-D373, but with sup45-36 ts instead of SUP45
Same as 33G-D373-r36 but with SUP35-C instead of SUP35
Same as in 33G-D373-rSL23, but with sup35::TRP1
Same as in 33G-D373-r36, but with sup35::TRP1
Same as in 33G-D373, but with sup45::ADE2
MAT a leu2-3,112 his3- Δ1 ura3-52 trp1-289 sup45::TRP1
Same as in s1-H8, but with SUP35-C instead of SUP35
Plasmids used in this study
Multicopy LEU2 vector
Centromeric LEU2 vector
Centromeric URA3 vector
Centromeric URA3 vector with SUP45
Same as pRS315, but with SUP45
Same as pRS315, but with the sup45-36 ts mutant allele
Same as pRS315, but with the sup45-sl23 ts mutant allele
Same as YEplac181, but the sup45-sl23 ts mutant allele
Same as pRS315, but with SUP35
Same as pRS315, but with SUP35-C11
Same as pRS315, but with SUP35-C14
Same as pRS315, but with SUP35-C42
Same as pRS316, but with SUP35
Integrative URA3 vector with SUP35-C
Integrative vector with the sup35::TRP1 disruption allele
Centromeric ADE3 URA3 vector containing SUP35
Same as pRS315, but with SUP35-C
Same as pRS315, but with SUP35-MC
Integrative URA3 vector with the sup45-sl23 ts allele
Integrative URA3 vector with the sup45-36 ts allele
Centromeric TRP1 vector containing SUP45 under the control of regulatable tetO2 promoter
The SUP45 gene was cloned using a yeast genomic library based on the plasmid p366, which was identical to YCp50 , but had LEU2 instead of URA3 (gift of P. Hieter). Isolation and sequencing of the sup45-sl23 ts allele was described earlier . The plasmid YEplac181-sup45-sl23 was obtained by insertion of the Xba I-Xho I DNA fragment of pRS315-sup45-sl23 containing the mutant sup45-sl23 ts allele into the Xba I and Sal I sites of YEplac181. The Xho I-Sac I DNA fragments carrying the sup45 mutant alleles of the plasmids pRS315-sup45-36 and pRS315-sup45-sl23 were cloned between the Sal I and Sac I sites of the integrative URA3 vector pJJ244  to obtain the plasmids pJJ244-sup45-36 and pJJ244-sup45-sl23, respectively. The pCM183-SUP45 plasmid bearing the SUP45 gene under the control of regulatable tetO2 promoter was constructed as follows. Using the plasmid pRS315-SUP45 as template, PCR was applied to place the Nco I site (CCATGG) at the SUP45 translation initiation codon. The obtained PCR fragment was digested with Nco I and Xho I and cloned into the same sites of the E. coli plasmid pET-30a(+) (Novagen) to generate the plasmid pL51-3. The Kpn I-Xho I fragment of pL51-3, containing the coding sequence of SUP45, was inserted into the Kpn I and Sal I sites of YEplac112 resulting in the YEplac112-atgSUP45 plasmid. Then, the Eco ICRI-Pst I fragment of YEplac112-atgSUP45 was cloned into the Hpa I and Pst I sites of pCM183 .
β-galactosidase activity in cell lysates was quantified as described . The levels of UAA, UAG and UGA readthrough were determined using the pUKC815/817/818/819 series vectors, as described previously .
Colony color sectoring assay
The assay is based on the facts that ade2 yeast cells accumulate a red pigment and form red colonies but ade2 ade3 cells do not accumulate this pigment and, consequently, form white colonies because the ade3 mutation blocks the pathway at a point prior to the accumulation of the pigment . Thus, a strain that is ade2 ade3, but carries ADE3 on an autonomously replicating plasmid, forms on medium non-selective for this plasmid red colonies (cells with the plasmid) that contain white sectors (cells that have lost the plasmid). We refer this phenotype as Sect+ (ability to lose the ADE3 plasmid), and, consequently, the phenotype of red clones without white sectors or with a negligible number of such sectors is Sect- (inability to lose the ADE3 plasmid). Multiple suspensions of the SUP35-C ade2 ade3 strain YJV159-rS35C carrying the centromeric plasmid pHT-SUP35 (SUP35 ADE3) were prepared in liquid uracil omission medium; each suspension deriving from a separate colony grown on uracil omission medium was diluted, and plated on ten YPDm plates at approximately 5,000 cells per plate. These cells were mutagenized with UV irradiation to approximately 10% survival and then incubated for 4 or 5 days. As expected, most clones were Sect+. Cells from Sect- colonies were streaked on YPDm and after 2 days, individual colonies were restreaked on YPDm. Only strains that continued to give red colonies or red colonies with rare white sectors were studied further.
We are grateful to P. Hieter for providing the yeast genomic library and G. Fominov for critical reading of the manuscript. This work was supported by grants from the Howard Hughes Medical Institute (grant 55000337) and Russian Foundation for Basic Research.
- Frolova L, Le Goff X, Rasmussen HH, Cheperegin S, Drugeon G, Kress M, Arman I, Haenni AL, Celis JE, Philippe M, Justesen J, Kisselev LL: A highly conserved eukaryotic protein family possessing properties of polypeptide chain release factor. Nature. 1994, 372: 701-703. 10.1038/372701a0View ArticlePubMedGoogle Scholar
- Zhouravleva G, Frolova L, Le Goff X, Le Guellec R, Inge-Vechtomov SG, Kisselev LL, Philippe M: Termination of translation in eukaryotes is governed by two interacting polypeptide chain release factors, eRF1 and eRF3. EMBO J. 1995, 14: 4065-4072.PubMed CentralPubMedGoogle Scholar
- Stansfield I, Jones KM, Kushnirov VV, Dagkesamanskaya AR, Poznyakovski AI, Paushkin SV, Nierras CR, Cox BS, Ter-Avanesyan MD, Tuite MF: The products of the SUP45 (eRF1) and SUP35 genes interact to mediate translation termination in Saccharomyces cerevisiae. EMBO J. 1995, 14: 4365-4373.PubMed CentralPubMedGoogle Scholar
- Frolova L, Le Goff X, Zhouravleva G, Davydova E, Philippe M, Kisselev L: Eukaryotic polypeptide chain release factor eRF3 is an eRF1- and ribosome-dependent guanosine triphosphatase. RNA. 1996, 2: 334-341.PubMed CentralPubMedGoogle Scholar
- Zavialov AV, Buckingham RH, Ehrenberg M: A posttermination ribosomal complex is the guanidine nucleotide exchange factor for peptide release factor RF3. Cell. 2001, 107: 115-124. 10.1016/S0092-8674(01)00508-6View ArticlePubMedGoogle Scholar
- Alkalaeva EZ, Pisarev AV, Frolova LY, Kisselev LL, Pestova TV: In vitro reconstitution of eukaryotic translation reveals cooperativity between release factors eRF1 and eRF3. Cell. 2006, 125: 1125-1136. 10.1016/j.cell.2006.04.035View ArticlePubMedGoogle Scholar
- Song H, Mugnier P, Das AK, Webb HM, Evans DR, Tuite MF, Hemmings BA, Barford D: The crystal structure of human eukaryotic release factor eRF1 – mechanism of stop codon recognition and peptidyl-tRNA hydrolysis. Cell. 2000, 100: 311-321. 10.1016/S0092-8674(00)80667-4View ArticlePubMedGoogle Scholar
- Ter-Avanesyan MD, Kushnirov VV, Dagkesamanskaya AR, Didichenko SA, Chernoff YO, Inge-Vechtomov SG, Smirnov VN: Deletion analysis of the SUP35 gene of the yeast Saccharomyces cerevisiae reveals two non-overlapping functional regions in the encoded protein. Mol Microbiol. 1993, 7: 683-692.View ArticlePubMedGoogle Scholar
- Ter-Avanesyan MD, Dagkesamanskaya AR, Kushnirov VV, Smirnov VN: The SUP35 omnipotent suppressor gene is involved in the maintenance of the non-Mendelian determinant [psi+] in the yeast Saccharomyces cerevisiae. Genetics. 1994, 137: 671-676.PubMed CentralPubMedGoogle Scholar
- Liu JJ, Sondheimer N, Lindquist SL: Changes in the middle region of Sup35 profoundly alter the nature of epigenetic inheritance for the yeast prion [PSI+]. Proc Natl Acad Sci USA. 2002, 99: 16446-16453. 10.1073/pnas.252652099PubMed CentralView ArticlePubMedGoogle Scholar
- Bradley ME, Liebman SW: The Sup35 domains required for maintenance of weak, strong or undifferentiated yeast [PSI+] prions. Mol Microbiol. 2004, 51: 1649-1659. 10.1111/j.1365-2958.2003.03955.xView ArticlePubMedGoogle Scholar
- Paushkin SV, Kushnirov VV, Smirnov VN, Ter-Avanesyan MD: Propagation of the yeast prion-like [PSI+] determinant is mediated by oligomerization of the SUP35 -encoded polypeptide chain release factor. EMBO J. 1996, 15: 3127-3134.PubMed CentralPubMedGoogle Scholar
- Patino MM, Liu JJ, Glover JR, Lindquist SL: Support for the prion hypothesis for inheritance of a phenotypic trait in yeast. Science. 1996, 273: 622-626.View ArticlePubMedGoogle Scholar
- Valouev IA, Kushnirov VV, Ter-Avanesyan MD: Yeast polypeptide chain release factors eRF1 and eRF3 are involved in cytoskeleton organization and cell cycle regulation. Cell Motil Cytoskeleton. 2002, 52: 161-173. 10.1002/cm.10040View ArticlePubMedGoogle Scholar
- Kong C, Ito K, Walsh MA, Wada M, Liu Y, Kumar S, Barford D, Nakamura Y, Song H: Crystal structure and functional analysis of the eukaryotic class II release factor eRF3 form S. pombe. Mol Cell. 2004, 14: 233-245. 10.1016/S1097-2765(04)00206-0View ArticlePubMedGoogle Scholar
- Gagny B, Silar P: Identification of the genes encoding the cytosolic translation release factors from Podospora anserina and analysis of their role during the life cycle. Genetics. 1988, 149: 1763-1775.Google Scholar
- Hoshino S, Imai M, Kobayashi T, Uchida N, Katada T: The eukaryotic polypeptide chain releasing factor (eRF3/GSPT) carrying the translation termination signal to the 3'-poly(A) tail of mRNA. Direct association of eRF3/GSPT with polyadenylate-binding protein. J Biol Chem. 1999, 274: 16677-16680. 10.1074/jbc.274.24.16677View ArticlePubMedGoogle Scholar
- Cosson B, Couturier A, Chabelskaya S, Kiktev D, Inge-Vechtomov S, Philippe M, Zhouravleva G: Poly(A)-binding protein acts in translation termination via eukaryotic release factor interaction and does not influence [PSI+] propagation. Mol Cell Biol. 2002, 22: 3301-3315. 10.1128/MCB.22.10.3301-3315.2002PubMed CentralView ArticlePubMedGoogle Scholar
- Hosoda N, Kobayashi T, Uchida N, Funakoshi Y, Kikuchi Y, Hoshino S, Katada T: Translation termination factor eRF3 mediates mRNA decay through the regulation of deanylation. J Biol Chem. 2003, 278: 28287-38291.Google Scholar
- Urakov VN, Valouev IA, Lewitin EI, Paushkin SV, Kosorukov VS, Kushnirov VV, Smirnov VN, Ter-Avanesyan MD: Itt1p, a novel protein inhibiting translation termination in Saccharomyces cerevisiae. BMC Mol Biol. 2001, 2: 9-18. 10.1186/1471-2199-2-9PubMed CentralView ArticlePubMedGoogle Scholar
- Bailleul PA, Newnam GP, Steenbergen JN, Chernoff YO: Genetic study of interactions between the cytoskeletal assembly protein Sla1 and prion-forming domain of the release factor Sup35 (eRF3) in Saccharomyces cerevisiae. Genetics. 1999, 153: 81-94.PubMed CentralPubMedGoogle Scholar
- Glover JR, Kowal AS, Schirmer EC, Patino MM, Liu JJ, Lindquist S: Self-seeded fibers formed by Sup35 the protein determinant of [PSI+], a heritable prion-like factor of S. cerevisiae. Cell. 1997, 89: 811-819. 10.1016/S0092-8674(00)80264-0View ArticlePubMedGoogle Scholar
- King C-Y, Tittmann P, Gross H, Gebert R, Aebi M, Wuthrich K: Prion-inducing domain 2-114 of yeast Sup35 protein transforms in vitro into amyloid-like filaments. Proc Natl Acad Sci USA. 1997, 94: 6618-6622. 10.1073/pnas.94.13.6618PubMed CentralView ArticlePubMedGoogle Scholar
- Breining P, Piepersberg W: Yeast omnipotent suppressor SUP1 (SUP45): nucleotide sequence of the wild type and a mutant gene. Nucleic Acids Res. 1986, 14: 5187-5197.PubMed CentralView ArticlePubMedGoogle Scholar
- Valouev IA, Urakov VN, Kochneva-Pervukhova NV, Ter-Avanesyan MD: Translation termination factors function outside of translation: yeast eRF1 interacts with myosin light chain, Mlc1p, to effect cytokinesis. Mol Microbiol. 2004, 53: 687-696. 10.1111/j.1365-2958.2004.04157.xView ArticlePubMedGoogle Scholar
- Ebihara K, Nakamura Y: C-terminal interaction of translational release factors eRF1 and eRF3 of fission yeast: G-domain uncoupled binding and the role of conserved amino acids. RNA. 1999, 5: 739-750. 10.1017/S135583829998216XPubMed CentralView ArticlePubMedGoogle Scholar
- Eurwilaichitr L, Graves FM, Stansfield I, Tuite MF: The C-terminus of eRF1 defines a functionally important domain for translation termination in Saccharomyces cerevisiae. Mol Microbiol. 1999, 32: 485-496. 10.1046/j.1365-2958.1999.01346.xView ArticlePubMedGoogle Scholar
- Paushkin SV, Kushnirov VV, Smirnov VN, Ter-Avanesyan MD: Interaction between yeast Sup45p (eRF1) and Sup35p (eRF3) polypeptide chain release factors: implications for prion-dependent regulation. Mol Cell Biol. 1997, 17: 2798-2805.PubMed CentralView ArticlePubMedGoogle Scholar
- Santoso A, Chien P, Osherovich LZ, Weissman JS: Molecular basis of a yeast prion species barrier. Cell. 2000, 100: 277-288. 10.1016/S0092-8674(00)81565-2View ArticlePubMedGoogle Scholar
- Kushnirov VV, Kochneva-Pervukhova NV, Chechenova MB, Frolova NS, Ter-Avanesyan MD: Prion properties of the Sup35 protein of yeast Pichia methanolica. EMBO J. 2000, 19: 324-331. 10.1093/emboj/19.3.324PubMed CentralView ArticlePubMedGoogle Scholar
- Chernoff YO, Galkin AP, Lewitin E, Chernova TA, Newnam GP, Belenkiy SM: Evolutionary conservation of prion-forming abilities of the yeast Sup35 protein. Mol Microbiol. 2000, 35: 865-876. 10.1046/j.1365-2958.2000.01761.xView ArticlePubMedGoogle Scholar
- Nakayashiki T, Ebihara K, Bannai H, Nakamura Y: Yeast [PSI+] "prions" that are crosstransmissible and susceptible beyond a species barrier through a quasi-prion state. Mol Cell. 2001, 7: 1121-1130. 10.1016/S1097-2765(01)00259-3View ArticlePubMedGoogle Scholar
- Hara H, Nakayashiki T, Crist CG, Nakamura Y: Prion domain interaction responsible for species discrimination in yeast [PSI+] transmission. Genes to Cells. 2004, 8: 925-939. 10.1111/j.1365-2443.2003.00694.x.View ArticleGoogle Scholar
- Sherman F, Fink GR, Hicks JB: Methods in yeast genetics. 1986, Cold Spring Harbor Laboratory PressGoogle Scholar
- Boeke JD, LaCroute F, Fink GR: A positive selection for mutants lacking orotidine-5'-phosphate decarboxylase activity in yeast: 5-fluoro-orotic acid resistance. Mol Gen Genet. 1984, 197: 345-346. 10.1007/BF00330984View ArticlePubMedGoogle Scholar
- Sambrook J, Fritsch EE, Maniatis T: Molecular Cloning: A Laboratory Manual. 1989, Cold Spring Harbor Laboratory Press, 2Google Scholar
- Gietz RD, Woods RA: Transformation of yeast by lithium acetate/single-stranded carrier DNA/polyethylene glycol method. Methods Enzymol. 2002, 350: 87-96.View ArticlePubMedGoogle Scholar
- Inoue H, Nojima H, Okayama H: High efficiency transformation of Escherichia coli with plasmids. Gene. 1990, 96: 23-28. 10.1016/0378-1119(90)90336-PView ArticlePubMedGoogle Scholar
- Lafontaine DLJ, Preiss T, Tollervey D: Yeast 18S rRNA dimethylase Dim1p: a quality control mechanism in ribosome synthesis?. Mol Cell Biol. 1998, 18: 2360-2370.PubMed CentralView ArticlePubMedGoogle Scholar
- Jones JS, Prakash L: Yeast Saccharomyces cerevisiae selectable markers in pUC18 polylinkers. Yeast. 1990, 6: 363-366. 10.1002/yea.320060502View ArticlePubMedGoogle Scholar
- Rose MD, Novick P, Thomas JH, Botstein D, Fink GR: A Saccharomyces cerevisiae genomic bank based on a centromere-containing shuttle vector. Gene. 1987, 60: 237-243. 10.1016/0378-1119(87)90232-0View ArticlePubMedGoogle Scholar
- Gari E, Piedrafita L, Aldea M, Herrero E: A set of vectors with tetracycline-regulatable promoter system for modulated gene expression in Saccharomyces cerevisiae. Yeast. 1997, 13: 837-848. 10.1002/(SICI)1097-0061(199707)13:9<837::AID-YEA145>3.0.CO;2-TView ArticlePubMedGoogle Scholar
- Miller JH: Experiments in Molecular Genetics. 1972, Cold Spring Harbor Laboratory PressGoogle Scholar
- Stansfield I, Akhmaloka , Tuite MF: A mutant allele of the SUP45 (SAL4) gene of Saccharomyces cerevisiae shows temperature-dependent allosuppressor and omnipotent suppressor phenotypes. Curr Genet. 1995, 27: 417-426. 10.1007/BF00311210View ArticlePubMedGoogle Scholar
- Roman H: Studies of gene mutation in Saccharomyces. Cold Spring Harbor Symp Quant Biol. 1956, 21: 175-185.View ArticlePubMedGoogle Scholar
- Fortes P, Kufel J, Fornerod M, Policarpou-Schwarz M, Lafontaine D, Tollervey D, Mattaj IW: Genetic and physical interactions involving the yeast nuclear cap-binding complex. Mol Cell Biol. 1999, 19: 6543-6553.PubMed CentralView ArticlePubMedGoogle Scholar
- Chernoff YO, Inge-Vechtomov SG, Derkach IL, Ptyushkina MV, Tarunina OV, Dagkesamanskaya AR, Ter-Avanesyan MD: Dosage-dependent translational suppression in yeast Saccharomyces cerevisiae. Yeast. 1992, 8: 489-499. 10.1002/yea.320080702View ArticlePubMedGoogle Scholar
- Gietz RD, Sugino A: New yeast- Escherichia coli shuttle vectors constructed with in vitro mutagenized yeast genes lacking six-base pair restriction sites. Gene. 1988, 74: 527-534. 10.1016/0378-1119(88)90185-0View ArticlePubMedGoogle Scholar
- Sikorski RS, Hieter P: A system of shuttle vectors and yeast host strains designed for efficient manipulation of DNA in Saccharomyces cerevisiae. Genetics. 1989, 122: 19-27.PubMed CentralPubMedGoogle Scholar
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