Figure 4
Compensatory mutations in plasmid SUP35-C restore viability of the strain carrying the sup45-36tsand SUP35-C alleles. The sup45-36tsmutant 33G-D373-r36-ΔS35 disrupted for SUP35 and carrying the SUP35 URA3 pRS316-SUP35 plasmid was transformed with either one of the LEU2 plasmids pRS315-SUP35 (SUP35), pRS315-SUP35C (SUP35-C), pRS315-SUP35-C11 (SUP35-C11), pRS315-SUP35-C14 (SUP35-C14), pRS315-SUP35-C42 (SUP35-C42) or pRS315 (empty vector). Transformants were incubated on 5FOA-containing medium for 3 days.