Figure 6

Interaction between the sup45^tsand SUP35-C alleles does not result in increase of the efficiency of nonsense codon readthrough. Data for the 33G-D373-rSL23 (sup45-sl23^tsSUP35) and 33G-D373-rSL23-r35C (sup45-sl23^tsSUP35-C) strains (A), as well as for 33G-D373-r36 (sup45-36^tsSUP35) and 33G-D373-r36-r35C (sup45-36^tsSUP35-C) strains (B) are presented. All strains carrying the pCM183-SUP45 plasmid (tetO₂-SUP45) were transformed with either one of the pUKC815, 817, 818, 819 plasmids and grown in tryptophane omission medium to mid log phase. Then the cells were collected, resuspended in analogous medium containing 10 μg/ml doxycycline. After 17 h of incubation in this medium, appropriate aliquots of yeast culture were taken and β-galactosidase activity was assayed. All data represent an average of at least three independent experiments.