Table 2 Plasmids used in this study

YEplac181

Multicopy LEU2 vector

[48]

pRS315

Centromeric LEU2 vector

[49]

pRS316

Centromeric URA3 vector

[49]

pRG415-SUP45

Centromeric URA3 vector with SUP45

[14]

pRS315-SUP45

Same as pRS315, but with SUP45

[25]

pRS315-sup45-36

Same as pRS315, but with the sup45-36^tsmutant allele

[25]

pRS315-sup45-sl23

Same as pRS315, but with the sup45-sl23^tsmutant allele

[25]

YEplac181-sup45-sl23

Same as YEplac181, but the sup45-sl23^tsmutant allele

This study

pRS315-SUP35

Same as pRS315, but with SUP35

This study

pRS315-SUP35-C11

Same as pRS315, but with SUP35-C11

This study

pRS315-SUP35-C14

Same as pRS315, but with SUP35-C14

This study

pRS315-SUP35-C42

Same as pRS315, but with SUP35-C42

This study

pRS316-SUP35

Same as pRS316, but with SUP35

This study

pFL44-SUP35C

Integrative URA3 vector with SUP35-C

[8]

pSUP35::TRP1

Integrative vector with the sup35::TRP1 disruption allele

This study

pHT-SUP35

Centromeric ADE3 URA3 vector containing SUP35

This study

pRS315-SUP35C

Same as pRS315, but with SUP35-C

This study

pRS315-SUP35MC

Same as pRS315, but with SUP35-MC

This study

pJJ244-sup45-sl23

Integrative URA3 vector with the sup45-sl23^tsallele

This study

pJJ244-sup45-36

Integrative URA3 vector with the sup45-36^tsallele

This study

pCM183-SUP45

Centromeric TRP1 vector containing SUP45 under the control of regulatable tetO₂ promoter

This study