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Table 1 S cerevisiae strains used in this study

From: N-terminal region of Saccharomyces cerevisiae eRF3 is essential for the functioning of the eRF1/eRF3 complex beyond translation termination

Strain

Genotype

Source/reference

YJV159

MAT a ade2 ade3 his3 leu2-3,112 trp1 ura3

[46]

YJV159-rS35C

Same as in YJV159, but with SUP35-C instead of SUP35

This study

YJV159-rS35C-SL23

Same as in YJV159-rS35C, but with sup45-sl23tsinstead of SUP45

This study

33G-D373

MAT a ura3-52 leu2-3 112 trp1-289 his7-1 lys9-A21 pheA10 ade2-144, 717

[47]

33G-D373-rSL23

Same as in 33G-D373, but with sup45-sl23tsinstead of SUP45

This study

33G-D373-rSL23-rS35C

Same as 33G-D373-rSL23 but with SUP35-C instead of SUP35

This study

33G-D373-r36

Same as in 33G-D373, but with sup45-36tsinstead of SUP45

This study

33G-D373-r36-rS35C

Same as 33G-D373-r36 but with SUP35-C instead of SUP35

This study

33G-D373-rSL23-ΔS35

Same as in 33G-D373-rSL23, but with sup35::TRP1

This study

33G-D373-r36-ΔS35

Same as in 33G-D373-r36, but with sup35::TRP1

This study

33G-D373-ΔS45

Same as in 33G-D373, but with sup45::ADE2

[25]

S1-H8

MAT a leu2-3,112 his3- Δ1 ura3-52 trp1-289 sup45::TRP1

[14]

S1-R-H8

Same as in s1-H8, but with SUP35-C instead of SUP35

[14]

  1. his7-1 and lys9-A21 and ade2-1, ochre mutations, trp1-289, amber mutation.