Hfq stimulates the activity of the CCA-adding enzyme
© Scheibe et al; licensee BioMed Central Ltd. 2007
Received: 26 January 2007
Accepted: 18 October 2007
Published: 18 October 2007
The bacterial Sm-like protein Hfq is known as an important regulator involved in many reactions of RNA metabolism. A prominent function of Hfq is the stimulation of RNA polyadenylation catalyzed by E. coli poly(A) polymerase I (PAP). As a member of the nucleotidyltransferase superfamily, this enzyme shares a high sequence similarity with an other representative of this family, the tRNA nucleotidyltransferase that synthesizes the 3'-terminal sequence C-C-A to all tRNAs (CCA-adding enzyme). Therefore, it was assumed that Hfq might not only influence the poly(A) polymerase in its specific activity, but also other, similar enzymes like the CCA-adding enzyme.
Based on the close evolutionary relation of these two nucleotidyltransferases, it was tested whether Hfq is a specific modulator acting exclusively on PAP or whether it also influences the activity of the CCA-adding enzyme. The obtained data indicate that the reaction catalyzed by this enzyme is substantially accelerated in the presence of Hfq. Furthermore, Hfq binds specifically to tRNA transcripts, which seems to be the prerequisite for the observed effect on CCA-addition.
The increase of the CCA-addition in the presence of Hfq suggests that this protein acts as a stimulating factor not only for PAP, but also for the CCA-adding enzyme. In both cases, Hfq interacts with RNA substrates, while a direct binding to the corresponding enzymes was not demonstrated up to now (although experimental data indicate a possible interaction of PAP and Hfq). So far, the basic principle of these stimulatory effects is not clear yet. In case of the CCA-adding enzyme, however, the presented data indicate that the complex between Hfq and tRNA substrate might enhance the product release from the enzyme.
Originally discovered as a host factor required for replication of phage Qβ RNA , Hfq (also named Host Factor I) has emerged as a multifunctional regulator with a variety of different targets in the bacterial cell. It forms a highly stable hexameric ring-shaped structure of identical subunits (11.2 kDa) that can polymerize as helical fibers . As a member of the Sm/Lsm protein family that is involved in RNA metabolism, Hfq contains an N-terminal α-helical domain followed by an antiparallel five-stranded beta-sheet, which is the hallmark of the so-called Sm domain . By its interaction with RNA, it controls the expression of many genes, stimulates the degradation of various mRNAs and modulates RNA processing events [4, 5]. Its chaperone activity plays an important role for interactions between regulatory small non-coding RNAs (sncRNA) and their target messenger RNAs [6–10]. Hfq preferentially binds to A/U- rich single stranded regions, especially to poly(A) tails and U-rich elements close to hairpin structures, where it induces structural changes . While the major part of Hfq is found in the cytoplasm, a certain amount binds to genomic DNA and is involved in nucleoid formation [12, 13].
Furthermore, Hfq stimulates the addition of poly(A) tails to mRNAs, where experimental data indicate that it interacts with RNA and possibly also with PAP [14, 15]. As a member of the nucleotidyltransferase family, this polymerase is closely related to tRNA nucleotidyltransferase (CCA-adding enzyme), an enzyme that catalyzes the addition of the CCA terminus to tRNA 3'-ends [16, 17]. Here, it is demonstrated that these two enzymes do not only share structural as well as functional elements [18, 19], but are also both modulated in their activity by Hfq.
Hfq stimulates CCA-addition
Interaction of Hfq with tRNA substrate
Interestingly, the observed interaction of Hfq with tRNA is not influenced by the presence or absence of a complete or partial CCA-terminus, since control gel shift experiments with tRNAPhe ending with C--, CC- or CCA showed that all transcript versions were bound with similar affinities (data not shown). This is in good agreement with the fact that the primary task of the CCA-adding enzyme in E. coli is the repair of such partial CCA termini, indicating a function of the Hfq-tRNA complex in the restoration of CCA termini.
Kinetic analysis of CCA-addition in presence or absence of Hfq or BSA. Apparent kinetic parameters of five independent experiments were determined and calculated in a nonlinear regression fit by using GraphPadPrism
0.31 ± 0.15
14.16 ± 2.16
0.11 ± 0.06
18.07 ± 2.01
0.36 ± 0.17
41.00 ± 6.52
Hfq is a multifunctional regulator in RNA metabolism of prokaryotes [4, 5, 36, 37]. One of its functions is to modulate RNA polyadenylation catalyzed by PAP [14, 15]. This enzyme is closely related to the CCA-adding enzyme that synthesizes and maintains the 3'-terminal CCA sequence on tRNAs. Due to sequence similarities, it is discussed that these enzymes share a common ancestor and interconverted into each other during evolution . This fact led to the question whether the activity of these related enzymes is also modulated by identical proteins. Since Hfq has a well characterized stimulating effect on PAP, it was therefore investigated whether it also influences the activity of the CCA-adding enzyme.
Here, it is shown that Hfq is indeed stimulating the CCA-adding enzyme from E. coli. While it is well known that BSA has a non-specific stabilizing effect on enzyme preparations, leading also to reaction enhancement, the Hfq effect cannot be explained by simple protein stabilization alone, since it significantly exceeds the influence of BSA. Other RNA binding proteins are also much less efficient than Hfq in stimulating CCA-addition (comparable to BSA), which is a further indication that Hfq exerts a specific effect. Hence, PAP as well as CCA-adding enzyme are both stimulated by this protein in their activities. The stimulation of polyadenylation is based on interaction of Hfq with the RNA substrate, where it binds as a hexameric ring of identical subunits to A/U-rich single-stranded elements closely located to hairpin-like structures [2, 7, 11, 38, 39]. This effect on polyadenylation was also observed in vivo, where Hfq might also interact with PAP [15, 40]. However, Hfq binding is not restricted to RNA transcripts carrying such structural recognition elements, but binds also to other, not yet identified sequences, as it was demonstrated for small regulatory RNA molecules and tRNAs, which is in agreement with our results . In the case of tRNA precursor transcripts carrying leader and trailer sequences, Hfq seems to regulate tRNA maturation by slowing down RNase E-catalyzed cleavage, as both proteins recognize similar elements . The tRNA transcripts, however, that were used as substrates for CCA-addition obviously do not carry typical Hfq binding motifs but belong to the pool of transcripts that interact with Hfq even in the absence of those elements. This tRNA – Hfq recognition is obviously mediated by amino acid residues of the protein that differ from those identified for interactions with mRNA or small regulatory RNAs. Indeed, replacement of V43 by R only affects stimulation of polyadenylation and interaction with mRNA , while substitution of K56 by A interferes with ncRNA stabilization . Yet, both variants do not affect the enhancement of CCA-addition.
Interestingly, a strong Hfq-specific stimulation in CCA-addition was only observed at tRNA concentrations at 0.25 μM and above, while at lower concentration, the presence of BSA led to a comparable enhancement. Since in this experiment the tRNA concentration was increased, while the Hfq concentration remained constant, the Hfq-tRNA ratio was dropping from 19:1 to 0.75:1 (for the monomer; 3:1 to 0.13:1 for the hexamer). This led to a significant increase of the reaction rate (which is not observed with BSA). Therefore, it is possible that at low tRNA concentrations, a higher number of Hfq hexamers binds to the tRNA and blocks the interaction with the CCA-adding enzyme, forming an inactive Hfq/tRNA complex which might reduce the CCA incorporation to some degree. This interpretation is supported by the observation of supershifted bands (consisting of tRNA and Hfq) at increased concentrations of Hfq in gel shift experiments with radioactively labeled tRNA, indicating the binding of further Hfq molecules to the tRNA (data not shown). The presence of such an excess of Hfq can interfere with enzyme – substrate interaction and is likely to lead to an increase of apparent KM values. Such inhibiting Hfq complexes were also observed with the small regulatory RNA DsrA, where stoichiometry of Hfq interaction determines the translatability of rpoS mRNA . At higher substrate tRNA concentrations, the inhibitory effect disappeared, presumably because less Hfq molecules bind to an individual tRNA, rendering it accessible for the CCA-adding enzyme.
How Hfq increases the reaction velocity but does not influence KM is not clear yet. Apparently, it exerts an indirect effect on the CCA-adding enzyme, since no supershift of tRNA, Hfq and CCA-adding enzyme was observed in the gel shift analysis (Fig. 2A), and the kinetic data indicate that the affinity of the CCA-adding enzyme to the tRNA is not affected by Hfq. The absence of such a supershifting complex shows that although such an interaction has to occur during the enhanced CCA-addition, it is not stable or persistent enough to be detected in the experiment. However, it is conceivable and in agreement with the absence of a supershift with CCA-adding enzyme that the Hfq/tRNA complex facilitates product release by weakening hydrogen bonds or ionic interactions between enzyme and tRNA. Subsequently, Hfq might also release the completed tRNA and cycle to a new substrate for CCA-addition. A similar dissociation of Hfq and RNA was already observed .
Taken together, it seems that Hfq is involved in the CCA-end restoration in E. coli, where about 15% of the tRNAs have a damaged 3'-end . The abundance of 10.000 Hfq hexamers per cell is likely to be sufficient to enhance the repair of these truncated CCA-termini . Additionally, there is growing evidence that Hfq-related Lsm-like proteins in other organisms are also participating in tRNA processing, as depletion of Lsm-like proteins in yeast led to the accumulation of several tRNA precursors . In this case the Lsm proteins obviously do not compete for tRNA binding (as Hfq and RNase E), but seem to recruit other processing factors to enhance tRNA maturation. Nevertheless, these data indicate that Lsm-like proteins (including Hfq) might have a general role in the processing pathway of tRNA transcripts.
The results reported here indicate that the multifunctional Lsm-like protein Hfq stimulates not only the activity of poly(A) polymerase, but also the reaction catalyzed by the CCA-adding enzyme, where it forms a complex with the tRNA substrate. The formation of this complex between Hfq and tRNA is particularly interesting, as it demonstrates a hitherto unknown specific and thermodynamically stable interaction of this protein with a mature tRNA transcript lacking the CCA-terminus. The kinetic analysis indicates that this complex does not affect the substrate affinity of the CCA-adding enzyme, but probably enhances the release of the reaction product, which is consistent with the fact that Hfq, tRNA and CCA-adding enzyme do not form a stable tertiary complex in the gel shift experiments.
Taken together, Hfq can stimulate the activity of the two closely related nucleotidyltransferases poly(A) polymerase and CCA-adding enzyme. It will be interesting to see whether – and how – this protein modulates also the activities of other members of this enzyme class.
tRNA substrates (unlabeled or internally labeled with α33P-UTP) with or without CCA terminus or carrying parts of it (-C, -CC) were produced by in vitro transcription . Transcripts were purified by denaturing polyacrylamide gel electrophoresis. Bands were cut out with a sterile blade, and tRNA was eluted by incubation in 0.5 M ammonium acetate (pH 5.7), 0.1 M EDTA, 1 mM MgCl2 and 0.1% (w/v) SDS at 4°C overnight. After ethanol precipitation, the transcript was dissolved in water, dephosphorylated  and purified by phenol-chloroform extraction.
Protein expression and purification
In vitroactivity assays
Up to 0.1 pmol CCA-adding enzyme were incubated in a total volume of 20 μl in the presence of NTPs (1 mM) and 5 pmol tRNA lacking a CCA terminus (internally labeled with α-33P-UTP) in 30 mM HEPES/KOH (pH 7.6), 30 mM KCl, 6 mM MgCl2 and 2 mM DTT at 30°C for varying incubation times. These assays contained 7.5 pmol Hfq or Hfq variants (calculated as monomer), 7.5 pmol BSA or 7.5 pmol RNA binding protein (HU, NusA, TGT, RNase P protein). After ethanol precipitation, reaction products were separated by denaturing gel electrophoresis and analyzed using a STORM 860 optical scanner and Image Quant software.
Gel shift experiments
5'-32P-labeled tRNA was incubated in presence or absence of CCA-adding enzyme, Hfq, or BSA (or combinations as indicated) under conditions described above. In control experiments for Hfq binding, competitor RNA was added in increasing amounts (20 to 600 fmol) to the 5'-32P-labeled tRNA (20 fmol). After addition of 5 μl loading dye (50% glycerol, 0.1% bromophenol blue, 0.1% xylene cyanol), RNA/protein complexes were separated on a native 6% polyacrylamide gel and visualized by autoradiography.
For the control experiments, the following transcripts were used:
T7 polymerase run-off transcript of an EcoRV-linearized pCR Topo 2.1 plasmid:
5'-GGG CGA AUU GGG CCC UCU AGA UGC AUG CUC GAG CGG CCG CCA GUG UGA UGG AU-3'
T7 polymerase run-off transcript (118 nt), corresponding to the 3'-part of the rpsO mRNA, including a poly(A) tail of 18 residues, carrying an Hfq binding site .
In vitro tRNA transcripts with complete or partial CCA ends .
For Michaelis-Menten kinetics, 0.1 pmol CCA-adding enzyme were tested in 20 μl reaction volumes with six data points in the reaction buffer (see above) containing 1 mM NTPs and 5 μCi α-32P-ATP (3000 Ci/mmol). Unlabeled tRNAPhe was titrated between 0.1 μM and 2.5 μM. Assays were incubated at 30°C for 7 min in the presence or absence of 37.5 pmol Hfq (calculated for the monomer) in order to avoid an underrepresentation of Hfq/tRNA complexes at higher RNA concentrations. An identical concentration was used for BSA. The reaction was stopped by ethanol precipitation and products were separated by denaturing polyacrylamide gel electrophoresis. Densitometric analysis of reaction products was carried out using a STORM 860 phosphor imager and ImageQuant software. Apparent kinetic parameters of 5 independent experiments were determined using GraphPad Prism software.
We would like to thank Christiane Rammelt and Andrea Just for critical reading of the manuscript and many helpful discussions. Furthermore, we thank Roland Hartmann for donation of RNase P, Josette Rouvière-Yaniv for HU protein, Stefan Vörtler and Stefan Pasch for NusA protein and Tina Ritschel, Stefanie Dörr, Klaus Reuter and Gerhard Klebe for the TGT protein preparation. The work was funded by the Deutsche Forschungsgemeinschaft (MO 634/2).
- Franze de Fernandez MT, Hayward WS, August JT: Bacterial proteins required for replication of phage Q ribonucleic acid. Pruification and properties of host factor I, a ribonucleic acid-binding protein. J Biol Chem. 1972, 247 (3): 824-831.PubMedGoogle Scholar
- Arluison V, Mura C, Guzman MR, Liquier J, Pellegrini O, Gingery M, Regnier P, Marco S: Three-dimensional structures of fibrillar Sm proteins: Hfq and other Sm-like proteins. J Mol Biol. 2006, 356 (1): 86-96. 10.1016/j.jmb.2005.11.010.View ArticlePubMedGoogle Scholar
- Sauter C, Basquin J, Suck D: Sm-like proteins in Eubacteria: the crystal structure of the Hfq protein from Escherichia coli. Nucleic Acids Res. 2003, 31 (14): 4091-4098. 10.1093/nar/gkg480.PubMed CentralView ArticlePubMedGoogle Scholar
- Vassilieva M, Garber MB: The regulatory role of the Hfq protein in bacterial cells. Mol Biol (Mosk). 2002, 36 (6): 970-977.View ArticleGoogle Scholar
- Valentin-Hansen P, Eriksen M, Udesen C: The bacterial Sm-like protein Hfq: a key player in RNA transactions. Mol Microbiol. 2004, 51 (6): 1525-1533. 10.1111/j.1365-2958.2003.03935.x.View ArticlePubMedGoogle Scholar
- Geissmann TA, Touati D: Hfq, a new chaperoning role: binding to messenger RNA determines access for small RNA regulator. EMBO J. 2004, 23 (2): 396-405. 10.1038/sj.emboj.7600058.PubMed CentralView ArticlePubMedGoogle Scholar
- Moller T, Franch T, Hojrup P, Keene DR, Bachinger HP, Brennan RG, Valentin-Hansen P: Hfq: a bacterial Sm-like protein that mediates RNA-RNA interaction. Mol Cell. 2002, 9 (1): 23-30. 10.1016/S1097-2765(01)00436-1.View ArticlePubMedGoogle Scholar
- Zhang A, Wassarman KM, Rosenow C, Tjaden BC, Storz G, Gottesman S: Global analysis of small RNA and mRNA targets of Hfq. Mol Microbiol. 2003, 50 (4): 1111-1124. 10.1046/j.1365-2958.2003.03734.x.View ArticlePubMedGoogle Scholar
- Rasmussen AA, Eriksen M, Gilany K, Udesen C, Franch T, Petersen C, Valentin-Hansen P: Regulation of ompA mRNA stability: the role of a small regulatory RNA in growth phase-dependent control. Mol Microbiol. 2005, 58 (5): 1421-1429.View ArticlePubMedGoogle Scholar
- Udekwu KI, Darfeuille F, Vogel J, Reimegard J, Holmqvist E, Wagner EG: Hfq-dependent regulation of OmpA synthesis is mediated by an antisense RNA. Genes Dev. 2005, 19 (19): 2355-2366. 10.1101/gad.354405.PubMed CentralView ArticlePubMedGoogle Scholar
- Folichon M, Arluison V, Pellegrini O, Huntzinger E, Regnier P, Hajnsdorf E: The poly(A) binding protein Hfq protects RNA from RNase E and exoribonucleolytic degradation. Nucleic Acids Res. 2003, 31 (24): 7302-7310. 10.1093/nar/gkg915.PubMed CentralView ArticlePubMedGoogle Scholar
- Azam TA, Hiraga S, Ishihama A: Two types of localization of the DNA-binding proteins within the Escherichia coli nucleoid. Genes Cells. 2000, 5 (8): 613-626. 10.1046/j.1365-2443.2000.00350.x.View ArticlePubMedGoogle Scholar
- Azam TA, Ishihama A: Twelve species of the nucleoid-associated protein from Escherichia coli. Sequence recognition specificity and DNA binding affinity. J Biol Chem. 1999, 274 (46): 33105-331013. 10.1074/jbc.274.46.33105.View ArticlePubMedGoogle Scholar
- Hajnsdorf E, Regnier P: Host factor Hfq of Escherichia coli stimulates elongation of poly(A) tails by poly(A) polymerase I. Proc Natl Acad Sci USA. 2000, 97 (4): 1501-1505. 10.1073/pnas.040549897.PubMed CentralView ArticlePubMedGoogle Scholar
- Mohanty BK, Maples VF, Kushner SR: The Sm-like protein Hfq regulates polyadenylation dependent mRNA decay in Escherichia coli. Mol Microbiol. 2004, 54 (4): 905-920. 10.1111/j.1365-2958.2004.04337.x.View ArticlePubMedGoogle Scholar
- Yue D, Maizels N, Weiner AM: CCA-adding enzymes and poly(A) polymerases are all members of the same nucleotidyltransferase superfamily: characterization of the CCA-adding enzyme from the archaeal hyperthermophile Sulfolobus shibatae. RNA. 1996, 2 (9): 895-908.PubMed CentralPubMedGoogle Scholar
- Schürer H, Schiffer S, Marchfelder A, Mörl M: This is the end: processing, editing and repair at the tRNA 3'-terminus. Biol Chem. 2001, 382 (8): 1147-1156. 10.1515/BC.2001.144.View ArticlePubMedGoogle Scholar
- Betat H, Rammelt C, Martin G, Mörl M: Exchange of Regions between Bacterial Poly(A) polymerase and CCA Adding Enzyme Generates Altered Specificities. Mol Cell. 2004, 15: 389-398. 10.1016/j.molcel.2004.06.026.View ArticlePubMedGoogle Scholar
- Reuven NB, Zhou Z, Deutscher MP: Functional overlap of tRNA nucleotidyltransferase, poly(A) polymerase I, and polynucleotide phosphorylase. J Biol Chem. 1997, 272 (52): 33255-33259. 10.1074/jbc.272.52.33255.View ArticlePubMedGoogle Scholar
- Hall KB, Sampson JR, Uhlenbeck OC, Redfield AG: Structure of an unmodified tRNA molecule. Biochemistry. 1989, 28 (14): 5794-5801. 10.1021/bi00440a014.View ArticlePubMedGoogle Scholar
- Robertus JD, Ladner JE, Finch JT, Rhodes D, Brown RS, Clark BF, Klug A: Structure of yeast phenylalanine tRNA at 3 A resolution. Nature. 1974, 250 (467): 546-551. 10.1038/250546a0.View ArticlePubMedGoogle Scholar
- Sampson JR, Uhlenbeck OC: Biochemical and physical characterization of an unmodified yeast phenylalanine transfer RNA transcribed in vitro. Proc Natl Acad Sci USA. 1988, 85 (4): 1033-1037. 10.1073/pnas.85.4.1033.PubMed CentralView ArticlePubMedGoogle Scholar
- Shi H, Moore PB: The crystal structure of yeast phenylalanine tRNA at 1.93 A resolution: a classic structure revisited. RNA. 2000, 6 (8): 1091-1105. 10.1017/S1355838200000364.PubMed CentralView ArticlePubMedGoogle Scholar
- Thomas BC, Chamberlain J, Engelke DR, Gegenheimer P: Evidence for an RNA-based catalytic mechanism in eukaryotic nuclear ribonuclease P. RNA. 2000, 6 (4): 554-562. 10.1017/S1355838200991477.PubMed CentralView ArticlePubMedGoogle Scholar
- Khvorova A, Motorin Y, Wolfson AD: Pyrophosphate mediates the effect of certain tRNA mutations on aminoacylation of yeast tRNA(Phe). Nucleic Acids Res. 1999, 27 (22): 4451-4456. 10.1093/nar/27.22.4451.PubMed CentralView ArticlePubMedGoogle Scholar
- Dale T, Sanderson LE, Uhlenbeck OC: The affinity of elongation factor Tu for an aminoacyl-tRNA is modulated by the esterified amino acid. Biochemistry. 2004, 43 (20): 6159-6166. 10.1021/bi036290o.View ArticlePubMedGoogle Scholar
- Greenblatt J, McLimont M, Hanly S: Termination of transcription by nusA gene protein of Escherichia coli. Nature. 1981, 292 (5820): 215-220. 10.1038/292215a0.View ArticlePubMedGoogle Scholar
- Reuter K, Ficner R: Sequence analysis and overexpression of the Zymomonas mobilis tgt gene encoding tRNA-guanine transglycosylase: purification and biochemical characterization of the enzyme. J Bacteriol. 1995, 177 (18): 5284-5288.PubMed CentralPubMedGoogle Scholar
- Buck AH, Kazantsev AV, Dalby AB, Pace NR: Structural perspective on the activation of RNAse P RNA by protein. Nature structural & molecular biology. 2005, 12 (11): 958-964.View ArticleGoogle Scholar
- Balandina A, Claret L, Hengge-Aronis R, Rouviere-Yaniv J: The Escherichia coli histone-like protein HU regulates rpoS translation. Molecular microbiology. 2001, 39 (4): 1069-1079. 10.1046/j.1365-2958.2001.02305.x.View ArticlePubMedGoogle Scholar
- Balandina A, Kamashev D, Rouviere-Yaniv J: The bacterial histone-like protein HU specifically recognizes similar structures in all nucleic acids. DNA, RNA, and their hybrids. J Biol Chem. 2002, 277 (31): 27622-27628. 10.1074/jbc.M201978200.View ArticlePubMedGoogle Scholar
- Mikulecky PJ, Kaw MK, Brescia CC, Takach JC, Sledjeski DD, Feig AL: Escherichia coli Hfq has distinct interaction surfaces for DsrA, rpoS and poly(A) RNAs. Nature structural & molecular biology. 2004, 11 (12): 1206-1214. 10.1038/nsmb858.View ArticleGoogle Scholar
- Ziolkowska K, Derreumaux P, Folichon M, Pellegrini O, Regnier P, Boni IV, Hajnsdorf E: Hfq variant with altered RNA binding functions. Nucleic acids research. 2006, 34 (2): 709-720. 10.1093/nar/gkj464.PubMed CentralView ArticlePubMedGoogle Scholar
- Sonnleitner E, Napetschnig J, Afonyushkin T, Ecker K, Vecerek B, Moll I, Kaberdin VR, Blasi U: Functional effects of variants of the RNA chaperone Hfq. Biochem Biophys Res Commun. 2004, 323 (3): 1017-1023. 10.1016/j.bbrc.2004.08.190.View ArticlePubMedGoogle Scholar
- Vecerek B, Moll I, Blasi U: Translational autocontrol of the Escherichia coli hfq RNA chaperone gene. RNA. 2005, 11 (6): 976-984. 10.1261/rna.2360205.PubMed CentralView ArticlePubMedGoogle Scholar
- Tsui HC, Leung HC, Winkler ME: Characterization of broadly pleiotropic phenotypes caused by an hfq insertion mutation in Escherichia coli K-12. Mol Microbiol. 1994, 13 (1): 35-49. 10.1111/j.1365-2958.1994.tb00400.x.View ArticlePubMedGoogle Scholar
- Muffler A, Traulsen DD, Fischer D, Lange R, Hengge-Aronis R: The RNA-binding protein HF-I plays a global regulatory role which is largely, but not exclusively, due to its role in expression of the sigmaS subunit of RNA polymerase in Escherichia coli. J Bacteriol. 1997, 179 (1): 297-300.PubMed CentralPubMedGoogle Scholar
- Zhang A, Wassarman KM, Ortega J, Steven AC, Storz G: The Sm-like Hfq protein increases OxyS RNA interaction with target mRNAs. Mol Cell. 2002, 9 (1): 11-22. 10.1016/S1097-2765(01)00437-3.View ArticlePubMedGoogle Scholar
- Folichon M, Allemand F, Regnier P, Hajnsdorf E: Stimulation of poly(A) synthesis by Escherichia coli poly(A)polymerase I is correlated with Hfq binding to poly(A) tails. The FEBS journal. 2005, 272 (2): 454-463. 10.1111/j.1742-4658.2004.04485.x.View ArticlePubMedGoogle Scholar
- Le Derout J, Folichon M, Briani F, Deho G, Regnier P, Hajnsdorf E: Hfq affects the length and the frequency of short oligo(A) tails at the 3' end of Escherichia coli rpsO mRNAs. Nucleic Acids Res. 2003, 31 (14): 4017-4023. 10.1093/nar/gkg456.PubMed CentralView ArticlePubMedGoogle Scholar
- Lease RA, Woodson SA: Cycling of the Sm-like protein Hfq on the DsrA small regulatory RNA. J Mol Biol. 2004, 344 (5): 1211-1223. 10.1016/j.jmb.2004.10.006.View ArticlePubMedGoogle Scholar
- Zhu L, Deutscher MP: tRNA nucleotidyltransferase is not essential for Escherichia coli viability. EMBO J. 1987, 6 (8): 2473-2477.PubMed CentralPubMedGoogle Scholar
- Kufel J, Allmang C, Verdone L, Beggs JD, Tollervey D: Lsm proteins are required for normal processing of pre-tRNAs and their efficient association with La-homologous protein Lhp1p. Mol Cell Biol. 2002, 22 (14): 5248-5256. 10.1128/MCB.22.14.5248-5256.2002.PubMed CentralView ArticlePubMedGoogle Scholar
- Mörl M, Lizano E, Willkomm DK, Hartmann RK: Production of RNAs with Homogeneous 5' and 3' Ends. Handbook of RNA Biochemistry. Edited by: Hartmann RK, Bindereif A, Schön A, Westhof E. 2005, Weinheim , Wiley-VCH, 1: 22-35.View ArticleGoogle Scholar
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