Figure 3
Apparent kinetic parameters of CCA-addition. Increasing amounts of unlabeled tRNAPhe were incubated with CCA-adding enzyme, NTPs and α-32P-ATP in presence (CCA+Hfq (blue diamonds), CCA+BSA (black triangles)) or absence (CCA, red bullets) of Hfq or BSA. Reaction products were separated by PAGE and analyzed by autoradiography. The corresponding reaction velocities were determined using GraphPadPrism software.