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Table 1 Kinetic analysis of CCA-addition in presence or absence of Hfq or BSA. Apparent kinetic parameters of five independent experiments were determined and calculated in a nonlinear regression fit by using GraphPadPrism

From: Hfq stimulates the activity of the CCA-adding enzyme

Enzyme Addition KM (μM) Vmax (nM/min)
CCA-adding enzyme - 0.31 ± 0.15 14.16 ± 2.16
CCA-adding enzyme BSA 0.11 ± 0.06 18.07 ± 2.01
CCA-adding enzyme Hfq 0.36 ± 0.17 41.00 ± 6.52