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Table 1 Kinetic analysis of CCA-addition in presence or absence of Hfq or BSA. Apparent kinetic parameters of five independent experiments were determined and calculated in a nonlinear regression fit by using GraphPadPrism

From: Hfq stimulates the activity of the CCA-adding enzyme

Enzyme

Addition

KM (μM)

Vmax (nM/min)

CCA-adding enzyme

-

0.31 ± 0.15

14.16 ± 2.16

CCA-adding enzyme

BSA

0.11 ± 0.06

18.07 ± 2.01

CCA-adding enzyme

Hfq

0.36 ± 0.17

41.00 ± 6.52