- Research article
- Open Access
Regulation of GAD65 expression by SMAR1 and p53 upon Streptozotocin treatment
BMC Molecular Biology volume 13, Article number: 28 (2012)
GAD65 (Glutamic acid decarboxylase 65 KDa isoform) is one of the most important auto-antigens involved in Type 1 diabetes induction. Although it serves as one of the first injury markers of β-islets, the mechanisms governing GAD65 expression remain poorly understood. Since the regulation of GAD65 is crucial for the proper functioning of insulin secreting cells, we investigated the stress induced regulation of GAD65 transcription.
The present study shows that SMAR1 regulates GAD65 expression at the transcription level. Using a novel protein-DNA pull-down assay, we show that SMAR1 binding is very specific to GAD65 promoter but not to the other isoform, GAD67. We show that Streptozotocin (STZ) mediated DNA damage leads to upregulation of SMAR1 and p53 expression, resulting in elevated levels of GAD65, in both cell lines as well as mouse β-islets. SMAR1 and p53 act synergistically to up-regulate GAD65 expression upon STZ treatment.
We propose a novel mechanism of GAD65 regulation by synergistic activities of SMAR1 and p53.
Type1 diabetes is an immunologically encountered autoimmune disease characterized by specific destruction of beta cells of islets of langerhans residing in pancreas . This specific response to beta cells is caused due to immune response against self molecules that behave as non-self (known as auto antigens). GAD (Glutamic acid decarboxylase) which is involved in synthesis of Gamma-Amino butyric acid or GABA, a neurotransmitter inhibitor [2, 3], is considered to be one of the strongest candidate auto antigens involved in triggering beta-cell-specific autoimmunity . Majority of recent type 1 diabetes patients and pre-diabetic subjects have anti-GAD antibodies in their sera, as do non-obese diabetic (NOD) mice, one of the best animal models for human type I diabetes. Immunization of young NOD mice with GAD results in prevention or delay of the disease as a result of tolerizing auto reactive T cells . On the other hand over-expression of GAD65 transgene in animal tissues can exacerbate the disease instead of tolerizing the animal . GAD usually occurs in two major isoforms; GAD65 and GAD67, encoded by two non-allelic genes located on different chromosomes  and are differentially regulated in various mammalian species. In beta cells of human and rat GAD65 is predominant, while in mouse though both the forms occur GAD67 predominates over GAD65 [6–8]. Although the expression level of GAD65 is extremely low in mouse β-islets, it is one of the major islet auto antigens [7–9]. Despite studies on the role of GAD65 in diabetes induction using various in-vitro and in-vivo models of spontaneous autoimmune diabetes [10, 11], the initial islet-specific factor(s) and the molecular mechanisms triggering the aberrant expression of GAD65 is still not very clear. The smaller form of the GAD auto-antigen namely GAD65, is a major target of humoral autoimmunity in type-1 diabetes. Recent data suggest that GAD65 expression in beta cells varies according to the functional state and the kind of stress . High glucose and glutamine have been shown to be putative positive regulators of GAD65 expression [13–15], while cytokines like IL-1 beta have been shown to act as negative regulators . GAD is an enzyme found in high concentration in the pancreas and brain where it catalyzes the conversion of glutamic acid to gamma-amino butyric acid (GABA). GABA is an inhibitory neurotransmitter that is important in the pancreas as a messenger between neurons and pancreatic cells [16, 17]. Specific experiments in animal models have shown that GAD expression is necessary for the autoimmune destruction of cells. One study in particular, performed on NOD mice (which are close to humans in their autoimmune manifestation of IDDM), incorporated an antisense GAD transgene into a subset of mice and found that IDDM development was significantly reduced .
Streptozotocin (STZ), derived from a fermentation broth of Streptomyces achromogenes, has been widely used to study the beta cell destruction both in-vitro and in-vivo models [19, 20]. The drug Streptozotocin (STZ) is a glucose analogue (N-[methylnitrosocarbamoyl]-D-glucosamine) that is rapidly transported into the β-cells via the glucose transporter, Glut2  and is known to be metabolized readily upon entry into the cell. The exact mechanism of STZ’s toxicity is not fully understood, but it has been suggested that its primary effect on beta cells is DNA damage by alkylation, unscheduled DNA synthesis, DNA adducts, chromosomal aberrations and DNA strand breaks induced by free radical generation [22, 23].
The tumor suppressor protein p53 can cause cell cycle arrest upon DNA damage induced activation [24–30]. In doing so, it facilitates the repair of damaged DNA or eliminates irreversibly damaged or abnormally growing cells to prevent potential transformation. Mdm2 helps to maintain steady state levels of p53 under normal condition through ubiquitination pathway [31–33]. STZ has also been shown to induce p53 protein in MIN6 cells  but the exact mechanism is largely unknown. One study also reported p53 antibodies circulating in patients suffering from type-1 diabetes .
Gene regulation is one of the most complex processes involving cross talk between a variety of proteins, nuclear matrix, DNA and many other DNA binding factors. The function of nuclear matrix is to provide a solid platform for efficient transcription. A number of matrix associated DNA region binding proteins (MARBPs) are known to be involved in regulation of transcription. SMAR1 is one such MARBP which was earlier shown to be involved in the regulation of Cyclin D1 and CK8 expression [36, 37]. SMAR1 specifically binds to putative MAR (MARβ) of the transcriptional enhancer (Eβ) at the T-cell receptor-β locus [38, 39]. This protein is known to cause cell cycle arrest and activates p53 through its serine 15 phosphorylation as well as through disruption of its interaction with MDM2 [40–43]. It is a potent tumor suppressor protein and significantly downregulated in higher grades of breast cancer .
The present study delineates a novel mechanism of regulation of GAD65 expression by two tumor suppressor proteins, SMAR1 and p53. We show that both the proteins can individually and synergistically upregulate GAD65 expression. We demonstrate that SMAR1 binds to GAD65 promoter in vitro and in-vivo to upregulate its mRNA expression. Our results show that STZ treatment leads to upregulation of SMAR1 and p53 expression. On the other hand MDM2 expression is downregulated leading to increased stability of p53. The stabilized p53 in turn binds to SMAR1 leading to an increased expression of GAD65. We also show a temporal increase of SMAR1 and p53 proteins on GAD65 promoter upon STZ treatment. Further studies using mouse β-islets confirmed our findings regarding the synergy between p53 and SMAR1 in GAD65 expression. Taken together, our results reveal a novel mechanism of regulation of major auto antigen GAD65 by SMAR1 and p53.
SMAR1 binds to multiple DNA sequences in genome
SMAR1 is documented to be a transcriptional activator/repressor in a context dependent manner, based on its DNA binding abilities. In vivo, SMAR1 binds to the promoters of Cyclin D1 and 5' UTR of cytokeratin 8 [36, 37]. To gain a better understanding of the transcription regulatory activities of SMAR1, we wanted to delineate various genomic DNA sequences bound by SMAR1. Therefore, we resorted to a novel pull down assay. Briefly, mouse genomic DNA was sheared to obtain small fragments of approximately 500 bp. These fragments were incubated with glutathione bead bound GST-SMAR1 recombinant protein and the bound DNA fragments were subsequently sequenced. Glutathione beads as well as bead bound GST were the controls used to negate non- specific binders. The resulting fragments were sequenced and then aligned using MEME software and a 50 mer consensus was derived (Figure 1A). Further manual alignments of the derived sequences lead to identification of a hexamer (TAATPu/Py Pu) consensus SMAR1 binding sequences where first four nucleotides are conserved while fifth one can be either pyrimidine or purine and the last nucleotide is either of the purines (Figure 1A, sequence shown in red). Further, we were able to validate our method by identification of some of the already known promoters bound by SMAR1, including cyclin D1 , Bax , 5' UTR of cytokeratin 8 . One of the unique promoters found to be bound by SMAR1 was GAD65 while the other isoform of GAD i.e. GAD67 promoter did not show any binding. In order to confirm that SMAR1 indeed binds to the GAD65 promoter, we performed CNBr pull down assay. The consensus sequence from GAD65 promoter and the general consensus oligonucleotide were individually coupled to CNBr beads and Rin 5f cell lysate was passed through the column. The protein samples were eluted from the CNBr columns and processed for Western blotting using SMAR1 specific antibody. Our result showed that SMAR1 bound with equal propensity to both GAD65 promoter sequence and the general consensus oligo (Figure 1B, lanes 4 and 5). In-vitro pull down assays followed by PCR amplification showed that GST-p53, 350–548 aa (DNA binding domain of SMAR1, 36) and the full-length SMAR1 were able to precipitate GAD65 promoter (Figure 1C, lanes 2, 3 & 4). The protein binding domain of SMAR1 i.e. GST-SMAR1 (160–350 aa), GST bound beads and glutathione beads alone did not show any amplification (Figure 1C, lanes 5–7) showing the specificity of the interaction. All these samples were further analyzed for GAD67 promoter binding. While we detected GAD67 promoter bound to GST-p53, neither the full length nor the DNA binding domain of SMAR1 showed any binding to GAD67 promoter (Figure 1D, lane 2). It is well known that p53 binds to GAD67 promoter . From the above mentioned results, it is clear that SMAR1 specifically binds to the consensus sequence in GAD65 promoter.
GAD65 has a TATA less promoter and various other factors are known to regulate it. Therefore we analyzed GAD65 promoter for other transcription factor binding sites in silico. A careful analysis of the sequence showed that SMAR1 binds 870 bp upstream of transcription start site. We found a strong p300 consensus element (~820 bp upstream) and a p53 binding site (~560 bp) juxtaposed to SMAR1 binding sites. A detailed map of various binding sites is shown in Additional file 1.
SMAR1 binds to GAD65 promoter and upregulates its expression
We further verified the binding of SMAR1 to GAD65 promoter using mobility shift assays. A 120 bp probe from GAD65 promoter which harbors the potential MAR and SMAR1 consensus binding site was radiolabelled and used for the assays. EMSA using radiolabelled GAD65 promoter probe showed a SMAR1-DNA complex formation (Figure 2A, lane 2) and a cold competitor reduced this complex formation (Figure 2A, lane 3) showing the specificity of binding. GAD67 and Actin (Figure 2B lanes 1–3 and 4–6 respectively) promoter specific probes did not show any complex formation with SMAR1 recombinant protein. Also, competition with cyclin D1 promoter oligo, greatly reduced the complex formation on GAD65 oligo and reflected the specificity of the complex formation (Figure 2C lane 3). Similarly, super-shift assays with SMAR1 specific antibody on using Rin cell lysate helped document SMAR1 complex formation on GAD65 promoter oligo (Figure 2D lane 2 and 3). The use of cold competitor in this experiment significantly reduced the specific complex formation (Figure 2D, lane 4).
After confirming that SMAR1 binds to GAD65 promoter, we proceeded to check the in vivo effect of SMAR1 binding on the promoter. It is known that GAD65 is the predominant form in rat while in mouse both the forms are expressed. Rat insuloma cell line Rin 5f cells were co-transfected with a luciferase reporter construct driven by GAD65 promoter, and expression plasmids/siRNAs of SMAR1 and p53. The results show that GAD65 promoter drives the expression of reporter gene upon over-expression of SMAR1 or p53 witnessed by an increase of ~ 4 and ~ 4.5 folds respectively (Figure 2E). On the other hand, knock-down of either of these proteins leads to a decreased luciferase activity driven by GAD65 promoter. Over-expression of SMAR1 and p53 together lead to the highest luciferase counts (~ 6 folds increase) indicating their additive effect on GAD65 promoter. On the other hand the knockdown of both lead to negligible promoter activity. Knock-down of p53 and over expression of SMAR1 partially rescued (~ 1.5 folds) the luciferase activity. These results indicate that although SMAR1 or p53 individually can up-regulate GAD65 promoter activity, their synergistic activity is required for maximal promoter activity that in turn reflects the transcriptional activation. On the other hand, either one of them is indispensible for activation of GAD65 promoter. It has been reported that phosphorylation of SMAR1 at serine 370 residue reduces its DNA binding activity [30; unpublished data]. Transfection of S370A mutant-SMAR1 led to a reduced GAD65 promoter activity compared to the wild-type SMAR1. This was not overcome by ectopic expression of p53 (Figure 2E, lanes 8 & 9 respectively). This result clearly indicates that direct binding of SMAR1 is essential for GAD65 promoter activation and that the effect of SMAR1 is not through stabilization/activation of p53. In order to verify our results we performed western blot analysis to confirm over expression as well as siRNA mediated knockdown of SMAR1 and p53. Figure 2F shows the expression levels of SMAR1 (upper panel) as well as p53 (lower panel) in Rin5f cells.
SMAR1 leads to upregulation of GAD65 expression
Next we verified the expression of GAD65 upon over expression of SMAR1. RT-PCR results showed that upon SMAR1 over expression, GAD65 and p53 mRNA levels are elevated in a dose dependent manner (Figure 3A). Densitometric analysis of the RT-PCR showed that upon transfection of 2 μg of SMAR1, GAD65 mRNA levels were 7 folds higher compared to the control cells, while p53 mRNA levels were increased by 3 folds (Figure 3B). Similarly, western blot analysis of the same showed increased GAD65 protein levels in a dose dependent over-expression of SMAR1. Earlier it has been reported that SMAR1 leads to phosphorylation of p53 at serine 15 and subsequent stabilization [41–43]. We also observed increased serine 15 phosphorylation as well as total p53 levels (Figure 3C). Actin was used as loading control. Also, our results showed that over expression of either of SMAR1 or p53 lead to elevated GAD65 protein levels while their knockdown decreased GAD65 expression (Figure 3D). We then transfected different truncations of SMAR1 pertaining to DNA binding (350–548 aa; F5) and protein interacting domain (160–350 aa; F6) of SMAR1 along with the full length SMAR1. Our results showed that full length as well as DNA binding domain of SMAR1 lead to increased expression of GAD65 (Figure 3E). Thus, our results show that DNA binding activity of SMAR1 is necessary for upregulation of GAD65 expression, while the protein interacting domain which has been shown to phosphorylate and activate p53 is not sufficient to drive p53 dependent expression of GAD65.
STZ induced DNA damage leads to upregulation of GAD65, SMAR1 and p53
It is known that GAD67 and p53 expression increases upon STZ treatment , which prompted us to verify the effect of STZ on SMAR1. As reported earlier, SMAR1 is a DNA damage responsive protein and is upregulated upon DNA damage induced by H2O2, γ-irradiation, Camptothecin and Doxorubicin. Our results here show that STZ treatment to Rin cells led to elevated mRNA levels of SMAR1, p53 and GAD65 in a dose dependent manner (Figure 4A). Western blotting of the same showed that SMAR1, p53 and GAD65 protein levels were elevated upon 4 mM STZ treatment. Interestingly Serine 15 as well as ser 46 phosphorylation of p53 also increases, indicating that prolonged STZ treatment leads to apoptosis through serine 46 phosphorylation of p53 (Figure 4B). FACS analysis of the cells also showed increased number of apoptotic cells upon STZ treatment (Figure 4C). Therefore, we examined the kinetics of SMAR1 and p53 binding to GAD65 promoter upon STZ treatment. Rin cells were treated with STZ for indicated time points and processed for ChIP analysis. Our results show that there is increased binding of SMAR1 as well as p53 on GAD65 promoter in a time dependent manner upon STZ treatment (Figure 4D). Histones on GAD65 promoter were hyper-acetylated in response to STZ treatment showing chromatin activation. The increase in recruitment of p300 to GAD65 promoter indicates chromatin activation that is reflected in increased GAD65 expression. On the other hand HDAC1 as well as Me3H4K20 showed time dependent loss of binding to GAD65 promoter (Figure 4E). HDAC1 recruitment causes local deacetylation of the chromatin thus leading to repressed state. Tri methylation of histone 4 lysine 20 residue is also indicative of the compact/repressed state of chromatin. All these results indicate that upon STZ treatment, SMAR1 and p53 binds to GAD65 promoter and recruit p300 which then leads to activation of GAD65 promoter. Upstream promoter region of GAD65 and actin promoter served as negative controls (Figure 4E, F).
Effect of STZ treatment on mouse β-islets
After verifying that SMAR1 leads to increased expression of GAD65 upon STZ induced DNA damage, we wanted to verify our results in-vivo. Mouse β-islets were isolated using collagen as detailed in materials and methods section and were cultured for 6 hrs prior to treatment. The islets were then treated with high (33 mM) and low (7 mM) dose of glucose, p53 activity inhibitor Pifithrin-α PFT and STZ (4 mM) for 24 hrs followed by confocal as well as RT-PCR analysis. Confocal results showed that STZ treatment leads to increased expression of SMAR1, p53 as well as GAD65 (Figure 5A & B). Phosphorylation of SMAR1 at serine 370 residue is also increased upon STZ treatment (Figure 5A). This post-translationally modified form of SMAR1 is known to have higher affinity for DNA . On the other hand the islets treated with PFT along with STZ did not show much change in GAD65 expression indicating that p53 is required for its higher expression.
RT-PCR in the islets showed that treatment with high glucose or STZ lead to increased expression of SMAR1, GAD65 and p53, while mdm2 mRNA levels are reduced (Figure 5C). Actin was used as loading control. On the other hand, islets treated with both p53 inhibitor PFT and STZ showed only a marginal increase in GAD65 expression indicating that although SMAR1 alone can up-regulate the GAD65 expression, the combinatorial effect of SMAR1 and p53 is needed for sustained levels of GAD65 (Figure 5C, lane 3). The densitometric analysis of RT-PCR shows that upon STZ treatment, p53, SMAR1 and GAD65 mRNA levels increase by ~2.1; ~2.7 & ~3.2 folds respectively (Figure 5D). This increased GAD65 is secreted in the circulation and is considered as the first injury marker for β-islet injury .
Transcription factors play an important role in regulation of gene expression through their DNA binding properties. In order to understand the different functions of a transcription factor, elucidation of its target genes is of utmost importance. As discussed earlier, SMAR1 is a transcription factor and is involved in various cellular pathways through regulation of target gene expression like Cyclin D1 , CK8  and Bax . In order to further delineate various promoters bound by SMAR1, we employed a novel in vitro binding assay using recombinant GST-SMAR1 protein. Our protocol involved in vitro binding of recombinant protein with sonicated mouse genomic DNA fragments. The high salt buffer maintains a stringent condition for protein-DNA interaction. Subsequent cloning and sequencing of pulled DNA fragments lead to validation of known SMAR1 target promoters as well as elucidation of many new promoter fragments, GAD65 being one of them. The interesting aspect of this interaction was that SMAR1 was specifically binding to only GAD65 and not to the promoter of other isoform GAD67. Since GAD65 is a crucial protein in the induction of diabetes, we investigated the mechanism of its regulation by SMAR1. The major phenotype in type-1 diabetes is apoptosis, resulting in a progressive loss of pancreatic beta cells through autoimmune attack. On the other hand it has been shown that over-expression of GAD65 transgene in animal tissues can exacerbate the disease instead of tolerizing the animal though the exact mechanism remains unclear . Another study using fetal mouse tissue, showed upregulation of GAD in pancreatic islet cells. This upregulation was shown to be caused by an impairment of mitochondrial complex I as a result of IDDM. Impairment of mitochondria resulted in an accumulation of glutamate that directly induced the upregulation of GAD in the cells . It is speculated that GAD expression on pancreatic beta cells may be involved in the modulation of beta cell specific autoimmune response and/or modulation of the functional state of beta cell . Much of the accumulated data from Type-1 animal models has come from the discovery of a novel diabetogenic compound Streptozotocin (STZ). The influence of STZ on the modulation of GAD expression has been shown earlier on GAD67 in MIN cells and also the INS cells treated with STZ show increased release of GABA in the culture medium that indirectly measures the rate of GAD expression [34, 48]. Several key transcriptional factors like NFκB and p53 have been shown to regulate the expression of GAD. Although it has been shown that STZ can regulate GAD67 but its effect on GAD65 expression has not been precisely evaluated .
In the present study we for the first time show that SMAR1 binds to GAD65 promoter but not GAD67. Using a novel in-vitro pull down assay we derived a consensus DNA binding site for SMAR1. Bioinformatics analysis of various known SMAR1 binding sequences revealed that there is significant similarity between DNA sequences where SMAR1 binds. For example, Cyclin D1 and GAD65 share the identical hexamer sequence in their promoter where SMAR1 binds. CNBr pull down assay followed by western blotting (South-western blotting) confirmed that SMAR1 binds to the consensus as well as GAD65 promoter. Binding of SMAR1 to GAD65 promoter was further verified using EMSA. Our results suggest that SMAR1 and p53 binds to GAD65 promoter and synergistically regulate its expression. Transient transfections of SMAR1 or p53 followed by RT-PCR as well as luciferase assays showed that SMAR1 as well as p53 individually can up-regulate GAD65 expression. On the other hand whenever transfected together, the GAD65 expression increased many fold, indicating that both SMAR1 and p53 act cumulatively leading to an increased expression of GAD65. Our results indicate that DNA binding domain binds to GAD65 promoter while protein interacting domain might recruit various other transcription factors leading to upregulation of GAD65 promoter.
In our model also we have investigated the possible role of STZ in modulation of GAD65 expression through co-ordinated interaction of p53 and SMAR1. We first examined the effect of STZ on the expression of GAD65, p53 and SMAR1 in rat beta cell line, RINm5f. We found that STZ significantly enhanced the expression of p53, SMAR1 and GAD65 molecules both at mRNA and protein level. This increased expression was observed in a time and dose dependent manner STZ treatment also led to increased phosphorylation of p53 at serine 15 as well as serine 46. Serine 15 phosphorylation of p53 indicates the activation of p53 while phosphorylation at serine 46 phosphorylation generally leads to apoptosis. FACS analysis also showed increased amount of apoptosis 36 h post STZ treatment. Collectively, our results showed that STZ treatment leads to upregulation of GAD65 expression ultimately leading to apoptosis. We further wanted to see the kinetics of binding of SMAR1 and p53 to GAD65 promoter upon STZ treatment. ChIP analysis showed that there is a time dependent increase in association of SMAR1 and p53 on GAD65 promoter. Markers for chromatin activation AcH3K9 also showed increased amplification. The increase in recruitment of p300 to GAD65 promoter indicates chromatin activation that is reflected in increased GAD65 expression. On the other hand HDAC1 as well as Me3H4K20 showed time dependent loss of binding to GAD65 promoter. All these results indicate that upon STZ treatment, SMAR1 and p53 binds to GAD65 promoter and recruits p300 which leads to activation of GAD65 promoter. We further verified our results using mouse β-islets. Our results showed that STZ mediated upregulation of GAD65 expression is dependent on conflated action of p53 and SMAR1.
Interestingly, the analysis of promoter sequences of GAD65 across species from Human, mouse, rat and found that SMAR1 bound sequence is rather conserved amongst these species. Further alignment of mouse GAD65 promoter sequences with guinea pig, monkey, Bos Taraus and chimpanzee genomes showed that there is a great degree of conservation amongst all these species. The detailed alignment study is described in Additional file 2.
Thus our study for the first time shows the involvement of a MAR binding protein SMAR1 in regulation of GAD65 expression. p53 is already known to bind to and regulate expression of GAD67 isoform of GAD. In the present study we show that p53 also regulates expression of GAD65. Upon STZ treatment SMAR1 and p53 leads to upregulation of GAD65 expression which may be secreted in the blood. This circulating GAD65 is considered as an early marker for β-cell injury . Taken together, our results show that GAD65 acts as one of the major injury marker as well as immuno-modulator. Thus regulation of GAD65 expression in β-islets by SMAR1 and p53 presents another facet of their involvement in DNA damage response.
The present study delineates yet another facet of regulation of GAD65 expression by two tumor suppressor proteins SMAR1 and p53 synergistically. The study describes a novel pull down assay as a method to build library of DNA fragments bound to a protein in-vitro. By employing various techniques like ChIP, south western assays to study kinetics of DNA-protein interactions, we show that SMAR1 and p53 binds to GAD65 promoter in close vicinity to each other and upregulate GAD65 expression in a time and dose dependent manner upon STZ treatment. Induction of stress to β-islets by streptozotocin causing activates p53 and SMAR1 which inturn bind to GAD65 promoter and upregulating its expression. This increased production of GAD65 protein may be secreted in blood and detected as injury/stress marker to β-islets during onset of diabetes.
In-vitro DNA pull down assay and sequence alignment
This assay is designed to use recombinant proteins to elucidate DNA fragments associated with the protein. GST-SMAR1 recombinant protein was purified as per the previously described protocol . The purity of recombinant protein was validated using 8% SDS-PAGE followed by silver stain. Mouse genomic DNA was sheared using Sonicator to obtain small fragments of approximately 500 bp. The purity and length of DNA was verified by running on 1.2% agarose gel. Just before the pull down reaction, the frozen recombinant protein was ultracentrifuged at 1, 00,000 rpm at 4°C to remove all the debris and unwanted protein aggregates. The DNA fragments were then incubated with glutathione bead bound GST-SMAR1 recombinant protein in DPD buffer (20 mM HEPES pH7.9, 1 mM EDTA, 30 mM KCl, 100 mM NaCl, 1 mM DTT, 25% Glycerol and 0.2% Tween-20) for 1 hrs at room temperature. The beads were than washed thrice with same buffer but increasing concentration of NaCl (100, 200, 300 mM NaCl) and bound DNA fragments were separated using phenol chloroform extraction. The fragments were than cloned into pGEMT easy vector and were subsequently sequenced using T7 specific primer. Glutathione beads as well as bead bound GST only were the controls used to rule out the non specific DNA binding of the recombinant protein. The resulting sequences were than aligned using MEME software and a 50mer consensus was derived.
Cell culture and treatments
For in-vitro assays, RINm5F cell line was provided by NCCS cell repository. RINm5F cells were cultured in RPMI medium with 10% FCS in CO2 incubator. 1 μg of SMAR1, p53 or pTU puro SMAR1 (SiRNA) DNA was used for transfection per well using lipofectamine. P53 siRNA was procured from Santa Cruz and was used as per manufacturer’s instructions. For in-vivo experiments 4–6 weeks old C57b6, SMAR1 transgenic and p53 null mice were kindly provided by NCCS animal house facility. The animal studies were conducted in accordance with Principles of Laboratory Animal Care. STZ was reconstituted in chilled phosphate citrate buffer, pH 4.5 and immediately used for treatments.
Immunoblotting and antibodies
Cells were scraped in 1XPBS and collected at indicated time points. Cells were then lysed using TNN buffer and protein concentration estimated using Bradford reagent (Bio-Rad). Equal amounts of proteins were taken for immunoblotting. Following sodium dodecyl sulphate (SDS)–polyacrylamide gel electrophoresis, the resolved proteins were electro blotted onto PVDF membrane (Amersham). The membrane was blocked overnight in Tris-buffered saline containing 0.1% Tween-20 (TBST) and 10% bovine serum albumin (BSA). The membrane was then probed with primary antibody in TBST for 2 h, followed by three 10-min TBST washes at room temperature. Incubation with the secondary antibody was done for 1 h, and three 10-min TBST washes were given prior to detection. Proteins were detected using enhanced chemiluminescence substrate (Amersham). The primary antibodies used were SMAR1 and S370 SMAR1 (Rabbit Polyclonal antibody raised in the lab; 49), p53 DO1 and actin (mouse monoclonal antibodies, Santa Cruz), ser15 p53 (#9284, Rabbit polyclonal; cell signaling), ser 46 p53(#2501, Rabbit Polyclonal; cell signaling), Ac 373/382 p53 (#2525, Rabbit Polyclonal; cell signaling) and GAD65 (mouse monoclonal antibody, #ab85866, Abcam). The secondary antibodies were donkey α-goat, donkey α-rabbit, donkey α-mouse (Bio-Rad).
Purification of GST fusion protein
GST-SMAR1 as well as GST (160–350) and GST (350–548) truncation clones of SMAR1 are different truncations of total SMAR1 protein having DNA binding and protein interaction domains respectively [36, 41]. All clones were grown in Luria-Bertani medium with ampicillin and induced with 1 mM isopropyl-D-thiogalactopyranoside (IPTG). Cells were resuspended in lysis buffer containing phosphate-buffered saline (PBS), Triton X-100, and protease inhibitors (Roche). After sonication and centrifugation, supernatant was incubated with glutathione Sepharose beads (Amersham) for 1 h at 4°C with gentle agitation. After three washes, with lysis buffer and PBS, the proteins were eluted at room temperature with 100 mM reduced glutathione buffer.
Electrophoretic mobility shift assay (EMSA)
For EMSA, oligonucleotide labelling was done by a Klenow reaction using [α32P] dCTP in a 20 μl reaction containing 1 mM dATG mix, Klenow buffer, and 0.5U of Klenow DNA polymerase (Invitrogen). Probe purification was done using Probequant G-50 column (Amersham Biosciences). Binding reactions were performed in a 10 μl total volume containing 10 mM HEPES (pH 7.9), 1 mM dithiothreitol, 50 mM KCl, 2.5 mM MgCl2, 10% glycerol, 0.5 to 1 μg double-stranded poly (dI-dC), 10 μg BSA and 1 μg of recombinant protein. Samples were incubated for 5 min at room temperature prior to addition of radiolabeled probe. The samples were then incubated for 15 min at room temperature, and the products of binding reactions were resolved using 8% native polyacrylamide gel electrophoresis. The gels were dried under vacuum and processed for autoradiography.
CNBr assay/Immunoaffinity purification
CNBr assay was done using previously described protocol by Nagore et al. . GAD65 promoter and control DNA fragments were amplified by PCR followed be gel elution for purity. The fragments were than coupled to CNBr-activated sepharose beads (Stratagene) using coupling buffer (0.1 M NaHCO3, 0.5 M NaCl, pH 8.3). The cell lysate was then passed at a slow rate through the column after washes with equilibration buffer. Proteins were eluted using increasing concentrations of NaCl, pH 8.0 in 0.1 M Tris–glycine buffer pH 2.5. All elutes were pooled and concentrated followed by Western blot using SMAR1 antibody.
Luciferase reporter assays
24 hours after transfection, the culture medium was removed, cells were washed with phosphate buffered saline (PBS), resuspended in 100 μl of cell lysis buffer. After freeze-thawing twice, lysed cells were spun at 9500 rpm for 15 min. Equal amount of protein was used for the assays. Luciferase activity was measured with Luclite substrate (Perkin Elmer, USA) and assay reactions read using Top-Count luminometer (Packard Life sciences, USA). Graphs were plotted from data obtained as a mean of 3 independent experiments along with computed standard deviations as error bars. The sequence of GAD65 promoter fragment used for luciferase assay is given in supplementary Figure 1.
Assays were performed using Chromatin Immunoprecipitation (ChIP) assay kit (Upstate Biotechnology) following manufacturer’s instructions. 1x106 cells were plated per 30 mm dish and treated with 3 mM STZ or left untreated. After treatment, DNA-protein interactions were fixed by adding formaldehyde directly to the media to a final concentration of 1%, incubated at 37°C for 10 min. Cells were washed with 1X PBS, pelletted and lysed in SDS-lysis buffer by sonication. The samples were then centrifuged at 15,000 rpm in order to clear the debris and chromatin extracts were incubated with 2 μg of the indicated antibody isotype control antibody and rotated at 4°C for 8–12 h. The antibody-chromatin complex was precipitated by adding protein A-sepharose bead, incubated for 4 h by rotating at 4°C and centrifuged at 3,000 rpm for 5 min. ChIP assays were carried out using SMAR1, HDAC1, acetyl Histone 3 Lysine-9 (H3K9), Histone 4 Lysine 16 (H4K16) and p53 antibodies (Cell Signaling). Input DNA, Rabbit IgG (r-IgG), and Mouse IgG (m-IgG) pulled DNA served as controls for all the experiments. Immunoprecipitated DNA was then subjected to 30 cycles of semi-quantitative PCR using the primers mentioned in Table 1.
Cell cycle analysis by FACS
DNA content of cells was measured to check apoptosis. 1x 106 RINm5F cells were seeded in a 6-well plate 24 hours before the day of experiment. RINm5F cells were treated with different concentrations of STZ 1, 2.5, 5 and 10 mM for 24 hours. The cells were fixed with cold 70% ethanol overnight and then treated with 10 μg/ml RNase at 37°C for 30 min. DNA content was measured by staining cells with propidium iodide (50 μg/ml) for 15 min. at RT. Apoptotic cells show low DNA stain ability resulting in a distinct, quantifiable region at sub-GO/G1 peak and analyzed by flow cytometry at 620 nm.
Pancreas was dissected out from the mice and minced with scissor. Then 1–2 washings were given with plain DMEM. The minced tissue was let to settle for some time and supernatant was removed carefully. In a conical flask islet dissociation medium containing collagenase (Roche) and soybean trypsin inhibitor (Sigma-Aldrich) was added on the tissue and the mixture was stirred for 20 minutes. An ice cold RPMI with 10% FCS was added to stop the reaction. Mixture was centrifuged at low speed and the pellet was suspended in plain RPMI. The suspension was vortexed to detach acinar cells from islets and centrifuged again. Now the pellet was suspended in RPMI and floating islets were carefully removed by hand picking under microscope and proceeded for further treatments. The purity of the islets was confirmed using insulin staining.
Total RNA from RINm5F and islets was isolated by Tri-reagent (Sigma-Aldrich) according to manufacturer’s protocol. In brief, RINm5F cells or islets were lysed in Tri reagent by pipeting several times and mixed with chloroform and centrifuged at 12,000 g for 15 minutes. The aqueous phase was removed and RNA was precipitated in isopropanol. The RNA was pelletted at 10,000 g for 10 minutes, washed with ethanol and centrifuged. The pellet was air dried and suspended in DEPC water. cDNA was synthesized using 5 μg RNA as template in presence of MMLV-RT, MMLV-RT buffer, 10 mM dNTPs, DTT and RNAse OUT (All reagents were purchased independently from Invitrogen).
Notkins AL: Immunologic and Genetic Factors in Type 1 Diabetes. J Biol Chem. 2002, 277: 43545-43548. 10.1074/jbc.R200012200
Wu H, Jin Y, Buddhala C, Osterhaus G, Cohen E, Jin H, Wei J, Davis K, Obata K, Wu JY: Role of glutamate decarboxylase (GAD) isoform, GAD65, in GABA synthesis and transport into synaptic vesicles-Evidence from GAD65-knockout mice studies. Brain Res. 2007, 18: 1154-
Walls AB, Nilsen LH, Eyjolfsson EM, Vestergaard HT, Hansen SL: Schousboe, Sonnewald U, Waagepetersen HS: GAD65 is essential for synthesis of GABA destined for tonic inhibition regulating epileptiform activity. J Neurochem. 2010, 115 (6): 1398-1408. 10.1111/j.1471-4159.2010.07043.x
Geng L, Solimena M, Flavell RA, Sherwin RS, Hayday AC: Widespread expression of an autoantigen-GAD65 transgene does not tolerize non-obese diabetic mice and can exacerbate disease. Proc Natl Acad Sci U S A. 1998, 95 (17): 10055-10060. 10.1073/pnas.95.17.10055
Erlander MG, Tillakaratne NJ, Feldblum S, Patel N, Tobin AJ: Two genes encode distinct glutamate decarboxylases. Neuron. 1991, 7: 91-100. 10.1016/0896-6273(91)90077-D
Kim J, Richter W, Aanstoot HJ, Shi Y, Fu Q, Rajotte R, Warnock G, Baekkeskov S: Differential expression of GAD65 and GAD67 in human, rat, and mouse pancreatic islets. Diabetes. 1993, 42 (12): 1799-1808. 10.2337/diabetes.42.12.1799
Velloso LA, Kämpe O, Eizirik DL, Hallberg A, Andersson A, Karlsson FA: Human autoantibodies react with glutamic acid decarboxylase antigen in human and rat but not in mouse pancreatic islets. Diabetologia. 1993, 36 (1): 39-46. 10.1007/BF00399091
Velloso LA, Eizirik DL, Karlsson FA, Kämpe O: Absence of autoantibodies against glutamate decarboxylase (GAD) in the non-obese diabetic (NOD) mouse and low expression of the enzyme in mouse islets. Clin Exp Immunol. 1994, 96 (1): 129-137.
Bridgett M, Cetkovic-Cvrlje M, O'Rourke R, Shi Y, Narayanswami S, Lambert J, Ramiya V, Baekkeskov S, Leiter EH: Differential protection in two transgenic lines of NOD/Lt mice hyperexpressing the autoantigen GAD65 in pancreatic beta-cells. Diabetes. 1998, 47 (12): 1848-1856. 10.2337/diabetes.47.12.1848
Kim KS, Kang Y, Choi SE, Kim JH, Kim HM, Sun B, Jun HS, Yoon JW: Modulation of glucocorticoid-induced GAD expression in pancreatic beta-cells by transcriptional activation of the GAD67 promoter and its possible effect on the development of diabetes. Diabetes. 2002, 51 (9): 2764-2772. 10.2337/diabetes.51.9.2764
Velloso LA, Kämpe O, Hallberg A, Christmanson L, Betsholtz C, Karlsson FA: Demonstration of GAD-65 as the main immunogenic isoform of glutamate decarboxylase in type 1 diabetes and determination of autoantibodies using a radioligand produced by eukaryotic expression. J Clin Invest. 1993, 91 (5): 2084-2090. 10.1172/JCI116431
Garrett B, Cullinan WE, James P: Region-Specific Regulation of Glutamic Acid Decarboxylase (GAD) mRNA Expression in Central Stress Circuits. The Journal of Neuroscience. 1998, 18 (15): 5938-5947.
Petersen JS, Rimvall K, Jørgensen PN, Hasselager E, Moody A, Hejnaes K, Clausen JT, Dyrberg T: Regulation of GAD expression in rat pancreatic islets and brain by gamma-vinyl-GABA andglucose. Diabetologia. 1998, 41 (5): 530-535. 10.1007/s001250050942
Chesselet MF, Mercugliano M, Soghomonian JJ, Salin P, Qin Y, Gonzales C: Regulation of glutamic acid decarboxylase gene expression in efferent neurons of the basal ganglia. Prog Brain Res. 1993, 99: 143-154.
Velloso LA, Björk E, Ballagi AE, Funa K, Andersson A, Kämpe O, Karlsson FA: Eizirik DL Regulation of GAD expression in islets of Langerhans occurs both at the mRNA and protein level. Mol Cell Endocrinol. 1994, 102 (1–2): 31-37.
Ujihara N, Daw K, Gianani R, Boel E, Yu L, Powers AC: Identification of glutamic acid decarboxylase autoantibody heterogeneity and epitope regions in type I diabetes. Diabetes. 1994, 43 (8): 968-975. 10.2337/diabetes.43.8.968
Rodriguez-Trelles FA: The neurotransmitter inhibitor GABA, the basis of the mechanism of action of several drugs affecting the central nervous system. An R Acad Nac Med (Madr). 1984, 101 (4): 431-452.
Yoon JW, Yoon CS, Lim HW, Huang QQ, Kang Y, Pyun KH, Hirasawa K, Sherwin RS, Jun HS: Control of Autoimmune Diabetes in NOD Mice by GAD Expression or Suppression in β Cells. Science. 1999, 284: 1184-1187.
Wiley PF, : Isolation and chemistry of streptozotocin. 1981, 3-18. Amesterdam, The Netherlands: Elsevier North Holland Biomedical Press
Bolzan AD, Bianchi MS: Genotoxicity of streptozotocin. Mutat Res. 2002, 512 (2–3): 121-134.
Wilson GL, Leiter EH: Streptozotocin interactions with pancreatic beta cells and the induction of insulin-dependent diabetes. Curr Top Microbiol Immunol. 1990, 156: 27-54. 10.1007/978-3-642-75239-1_3
Schnedl WJ, Ferber S, Johnson JH, Newgard CB: STZ transport and cytotoxicity. Specific enhancement in GLUT2-expressing cells. Diabetes. 2001, 43 (11): 1326-1333.
Takasu N, Komiya I, Asawa T, Nagasawa Y, Yamada T: Streptozocin- and alloxan-induced H2O2 generation and DNA fragmentation in pancreatic islets: H2O2 as mediator for DNA fragmentation. Diabetes. 1991, 40 (9): 1141-1145. 10.2337/diabetes.40.9.1141
Lakin ND, Jackson SP: Regulation of p53 in response to DNA damage. Oncogene. 1999, 18 (53): 7644-7655. 10.1038/sj.onc.1203015
Reich NC, Oren M, Levine AJ: Two distinct mechanisms regulate the levels of a cellular tumor antigen, p53. Mol Cell Biol. 1983, 3 (12): 2143-2150.
Hall PA: p53: The Challenge of Linking Basic Science and Patient Management. Oncologist. 1998, 3 (4): 218-224.
Karawajew L, Rhein P, Czerwony G, Ludwig WD: Stress-induced activation of the p53 tumor suppressor in leukemia cells and normal lymphocytes requires mitochondrial activity and reactive oxygen species. Blood. 2005, 105 (12): 4767-4775. 10.1182/blood-2004-09-3428
Bode AM, Dong Z: Post-translational modification of p53 in tumorigenesis. Nat Rev Cancer. 2004, 4 (10): 793-805. 10.1038/nrc1455
Fingerman IM, Briggs SD: p53-mediated transcriptional activation: from test tube to cell. Cell. 2004, 117 (6): 690-691. 10.1016/j.cell.2004.05.021
Thompson T, Tovar C, Yang H, Carvajal D, Vu BT, Xu Q, Wahl GM, Heimbrook DC, Vassilev LT: Phosphorylation of p53 on key serines is dispensable for transcriptional activation and apoptosis. J Biol Chem. 2004, 279 (51): 53015-53022. 10.1074/jbc.M410233200
Klein C, Vassilev LT: Targeting the p53-MDM2 interaction to treat cancer. Br J Cancer. 2004, 91 (8): 1415-1419.
Saito A, Hayashi T, Okuno S, Nishi T, Chan PH: Modulation of p53 degradation via MDM2-mediated ubiquitylation and the ubiquitin-proteasome system during reperfusion after stroke: role of oxidative stress. J Cereb Blood Flow Metab. 2005, 25 (2): 267-280. 10.1038/sj.jcbfm.9600028
Stommel JM, Wahl GM: A New Twist in the Feedback Loop: Stress-Activated MDM2 Destabilization is required for p53 Activation. Cell Cycle. 2005, 4 (3): 411-417. 10.4161/cc.4.3.1522
Choi SE, Noh HL, Kim HM, Yoon JW, Kang Y: Streptozotocin upregulates GAD67 expression in MIN6N8a mouse beta cells. J Autoimmun. 2002, 19 (1–2): 1-8.
Di Cesare E, Previti M, Lombardo F, Di Benedetto A, Mazzu N, Romano G, De Luca F, Lasco A, Cucinotta D: Serum anti-p53 autoantibodies in patients with type 1 diabetes. Ann Clin Lab Sci. 2001, 31 (3): 253-258.
Rampalli S, Pavithra L, Bhatt A, Kundu TK, Chattopadhyay S: Tumor suppressor SMAR1 mediates cyclin D1 repression by recruitment of the SIN3/histone deacetylase 1 complex. Mol Cell Biol. 2005, 25 (19): 8415-8429. 10.1128/MCB.25.19.8415-8429.2005
Pavithra L, Singh S, Sreenath K, Chattopadhyay S: Tumor suppressor SMAR1 downregulates Cytokeratin 8 expression by displacing p53 from its cognate site. Int J Biochem Cell Biol. 2009, 41 (4): 862-871. 10.1016/j.biocel.2008.08.038
Chattopadhyay S, Kaul R, Charest A, Housman D, Chen J: SMAR1, a novel, alternatively spliced gene product, binds the Scaffold/Matrix-associated region at the T cell receptor beta locus. Genomics. 2000, 68 (1): 93-96. 10.1006/geno.2000.6279
Kaul-Ghanekar R, Jalota A, Pavithra L, Tucker P, Chattopadhyay S: SMAR1 and Cux/CDP modulate chromatin and act as negative regulators of the TCR beta enhancer (Ebeta). Nucleic Acids Res. 2004, 32 (16): 4862-4875. 10.1093/nar/gkh807
Kaul R, Mukherjee S, Ahmed F, Bhat MK, Chhipa R, Galande S, Chattopadhyay S: Direct interaction with and activation of p53 by SMAR1 retards cell-cycle progression at G2/M phase and delays tumor growth in mice. Int J Cancer. 2003, 103 (5): 606-615. 10.1002/ijc.10881
Jalota A, Singh K, Pavithra L, Kaul R, Jameel S, Chattopadhyay S: Tumor suppressor SMAR1 activates and stabilizes p53 through its arginine-serine (RS) rich motif. J Biol Chem. 2005, 280 (16): 16019-16029. 10.1074/jbc.M413200200
Jalota-Badhwar A, Kaul-Ghanekar R, Mogare D, Boppana R, Paknikar KM, Chattopadhyay S: SMAR1-derived P44 peptide retains its tumor suppressor function through modulation of p53. J Biol Chem. 2007, 282 (13): 9902-9913. 10.1074/jbc.M608434200
Pavithra L, Mukherjee S, Sreenath K, Kar S, Sakaguchi K, Roy S, Chattopadhyay S: SMAR1 forms a ternary complex with p53-MDM2 and negatively regulates p53-mediated transcription. J Mol Biol. 2009, 388 (4): 691-702. 10.1016/j.jmb.2009.03.033
Singh K, Mogare D, Giridharagopalan RO, Gogiraju R, Pande G, Chattopadhyay S: p53 target gene SMAR1 is dysregulated in breast cancer: its role in cancer cell migration and invasion. PLoS One. 2007, 2 (7): e660-
Myers MA, Georgiou HM, Byron S, Esposti MD: Inhibition of Mitochondrial Oxidative Phosphorylation Induces Hyper-Expression of Glutamic Acid Decarboxylase in Pancreatic Islet Cells. Autoimmunity. 1999, 30 (1): 43-51. 10.3109/08916939908994759
Sinha S, Malonia SK, Mittal SPK, Singh K, Kadreppa S, Kamat R, Mukhopadhyaya R, Pal JK, Chattopadhyay S: Coordinated regulation of p53 apoptotic targets BAX and PUMA by SMAR1 through an identical MAR element. EMBO Journal. 2010, 29 (4): 830-842. 10.1038/emboj.2009.395
Hinke SA: Finding GAD: Early Detection of ß-Cell Injury. Endocrinology. 2007, 148 (10): 4568-4571. 10.1210/en.2007-0861
Antony S, Kumar TP, Kuruvilla KP, George N, Paulose CS: Decreased GABA receptor binding in the cerebral cortex of insulin induced hypoglycemic and streptozotocin induced diabetic rats. Neurochem Res. 2010, 35 (10): 1516-1521. 10.1007/s11064-010-0210-7
Singh S, Sreenath K, Pavithra L, Roy S, Chattopadhyay S: SMAR1 regulates free radical stress through modulation of AKR1a4 enzyme activity. Int J Biochem Cell Biol. 2010, 42 (7): 1105-1114. 10.1016/j.biocel.2010.01.022
Nagore LN, Mitra S, Jiang D, Jiang S, Zhou YW, Loranc M, Jarrett HW: Cyanogen bromide-activated coupling: DNA catalytic chromatography purification of EcoRI endonucleases. Nature Protocols. 2007, 1: 2909-2915. 10.1038/nprot.2006.439. 10.1038/nprot.2006.439
This work was carried out at National Center for Cell Science (NCCS), DBT, India. We are thankful to Director, NCCS, Dr. G. C. Mishra for allowing us to complete this work. SS & PLC were recipients of UGC-SRF while VR & SK was recipient of CSIR-SRF. TD was a summer trainee in lab of SC. SS is also thankful to Central University of Punjab, Bathinda, India where manuscript was revised.
The authors declare that they have no competing interests.
SS, VR, PP and SC have designed all the experiments. SS, TD, PLC, SK and VR have performed and analyzed all the experiments. Manuscript has been prepared by SS, PLC and SC. All authors read and approved the final manuscript.
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