Figure 5
From: Regulation of GAD65 expression by SMAR1 and p53 upon Streptozotocin treatment
STZ treatment in mouse β-islets leads to upregulation of SMAR1, p53 and GAD65 expression. A &B. β-islets were treated with STZ and PFT individually as well as in combination followed by fluorescent staining using S370-SMAR1 (phosphor SMAR1 at S370 locus), total SMAR1, P53 and GAD65 antibodies. DAPI was used as nuclear marker to distinguish between cytoplasm and the nucleus. C. RT-PCR analysis of SMAR1, p53 and GAD65 upon streptozotocin (STZ), pifithrin-α (PFT) and PFT + STZ treatments. Actin was used as internal loading control. D. The same RT-PCR data was used for densitometric analysis. The graph indicates the respective expression of GAD65, p53 and SMAR1 expression at different treatments while the expression in control cells is taken as 1. Thus these values indicate expression comparison of a single molecule with different treatments and cannot be used to compare expression of two different genes.