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Fig. 2 | BMC Molecular Biology

Fig. 2

From: pH-mediated upregulation of AQP1 gene expression through the Spi-B transcription factor

Fig. 2

Identification of the AQP1 gene upstream promoter region. a, c and e Schematic representation of human AQP1 promoter reporter constructs. Fragments with various lengths of the AQP1 promoter region between − 4000 to 0 bp were cloned downstream of the firefly luciferase reporter. b, d and f Luciferase activity in HEK-293T cells transfected with firefly luciferase reporter plasmids containing various AQP1 upstream regions. The Renilla luciferase reporter was co-transfected with pGL3-basic or a plasmid reporter. The data represent the mean ± SEM. **P < 0.01; ***P < 0.001

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