Fig. 3
From: Interaction between NFATc2 and the transcription factor Sp1 in pancreatic carcinoma cells PaTu 8988t
Co-localization of NFATc2 and the transcription factor Sp1. PaTu 8988t cells were transiently transfected with NFATc2-GFP. The cells either remained in serum containing medium or, after 24 h, serum was removed for 3 h, and the cells were stimulated with 0.5 µM of Ionomycin for 0, 10, 30, or 60 min. The cells were fixed into paraformaldehyde and incubated with the Sp1 antibody. Cell nuclei (blue) are stained with DAPI, endogenous Sp1 (red) with alexarot, and NFATc2-GFP green