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Fig. 1 | BMC Molecular Biology

Fig. 1

From: Binding affinity of five PBPs to Ostrinia sex pheromones

Fig. 1

SDS-PAGE analysis of recombinant Ostrinia furnacalis pheromone binding proteins (PBPs) induced from E. coli clones carrying in frame insertions of OfurPBP1, OfurPBP2, OfurPBP3, OfurPBP4, or OfurPBP5 coding sequences in the pET30a(+) expression vector. Replicate gel lanes correspond to (1) cell culture prior to addition of isopropyl-β-d-1-thiogalactopyranoside (IPTG), (2) IPTG induced cells at O.D. 0.6–0.8, (3) supernatant after ultrasonic treatment and centrifugation, (4) pellet after ultrasonic treatment and centrifugation, (5) Ni–NTA column purified proteins, and (6) enterokinase digested products with His-tags cleaved. M corresponds to molecular weight marker. a OfurPBP1; b OfurPBP2; c OfurPBP3; d OfurPBP4; e OfurPBP5

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