Figure 2
Assessing the impact of the fluorescence threshold on slope-derived determination of amplification efficiency. (A) Amplification profiles taken from Figure 1A with the red lines representing the fluorescence thresholds that were manually set to values that span the entire height of the amplification profiles. (B) Standard curves generated from each of the five Ft settings (increasing from left to right). The numerical inlay summarizes the linear regression analysis, which indicates that Ft has no major impact on either r2 or Eslope until placed into the extreme upper region of the amplification profiles.