Figure 5

EMSA demonstrates binding of ΔNp63 to p63 binding sites located within the ChIPed DNA sequences. (A) EMSAs were performed by radiolabeling oligonucleotide probes containing p63 binding sites from the various ChIPed DNA sequences corresponding to known genes as indicated above each lane. Two radiolabeled oligonucleotide probes from each ChIPed sequence were each incubated with recombinant His-tagged ΔNp63α protein. Arrow indicates ΔNp63α. (B) EMSA was performed with nuclear extract from HaCaT cells and incubated with the radiolabeled oligonucleotide probe that bound best to recombinant protein demonstrated in A above. A complex containing ΔNp63 is supershifted with an anti-ΔNp63 antibody. See Additional File 1 for oligonucleotide sequences used in EMSA.