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Figure 7 | BMC Molecular Biology

Figure 7

From: Down-regulation of human topoisomerase IIα expression correlates with relative amounts of specificity factors Sp1 and Sp3 bound at proximal and distal promoter regions

Figure 7

The proximal topoisomerase IIα promoter forms a loop between GC1 and GC2. A ligation product (429 bp) was detected by PCR (lane 2) after completion of the 3C assay. Digestion of ligated product (lane 1) with BSu 36 I yielded two fragments (123 bp and 306 bp). The PCR product from the uncut genomic DNA control (lane 4) was 571 bp in size. When digested with BSu 36 I, genomic DNA product (lane 3) yielded three fragments (123, 306 and 142 bp) as predicted from the DNA sequence.

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BMC Molecular Biology

ISSN: 1471-2199

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