Figure 1

The luciferase assay evaluates polyadenylation efficiency. ATCC 3T3 cells (mouse embryonic fibroblasts) were transfected with Renilla luciferase reporter plasmids containing one of the indicated (bottom) polyadenylation cassettes as well as a firefly luciferase expressing vector to control for transfection efficiency. Two days later, extracts of these cells were assayed for luciferase activity. Indicated on the left is the relative luciferase activity (Renilla luciferase values divided by firefly luciferase values). Asterisks indicate significant difference from the SV40 wild type as determined by Student's T-test (p < 0.01).