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Table 2 Percentage GFP positive cells over time in shRNA-expressing IS-1 and HeLa cells. Transduced cells were assessed for GFP expression by flow cytometry. GFP positive cells were gated equally for each cell type, 4, 8 and 11 days after transduction with EGFP, sh319 and sh319scr vectors.

From: Short-term cytotoxic effects and long-term instability of RNAi delivered using lentiviral vectors

 

% GFP positive cells

 

IS-1

HeLa

Vector

Day 4

Day 8

Day 11

Day 4

Day 8

Day 11

EGFP

95

95

93

92

94

93

sh319

86

70

56

79

68

50

sh319scr

90

76

59

82

64

45