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Table 2 Nonsense mutations in SUP45 lead to decreased ascospore viability

From: Viable nonsense mutants for the essential gene SUP45 of Saccharomyces cerevisiae

D1628 transformed with plasmids: Plasmid stability % Total number of tetrads Tetrads with indicated number of viable spores % of His+Ura+ segregants a
    4 3 2 1  
pRS316/SUP45 71 49 7 19 22 1 27
pRS316/sup45-101 74 52 6 6 38 2 15
pRS316/sup45-102 71 30 1 1 26 2 5
pRS316/sup45-104 74 48 0 2 42 4 2
pRS316/sup45-105 72 35 0 0 32 3 0
pRS316/sup45-107 69 31 3 5 21 2 14
  1. a The amount of His+Ura+ segregants is a characteristic of sup45-n allele presence (His+ is the marker for the SUP45 gene disruption and Ura+- for the pRS316 plasmid presence). The data correspond to the sum of two experiments but the cell number in nonviable colonies was calculated from only one (Table 3).