Skip to main content

Table 1 The efficiency of maintenance of pRS315/SUP45 plasmid on SUP45::HIS3 [sup45-n] background

From: Viable nonsense mutants for the essential gene SUP45 of Saccharomyces cerevisiae

Transformants of 1A-D1628 bearing plasmids a: % of Ura+ Leu- colonies after rounds of selection:
pRS315 [LEU2] with: pRS316 [URA3] with: I b II c
SUP45 SUP45 9.5 ± 1.1 11.0 ± 0.7
SUP45 sup45-101 6.3 ± 0.8 10.9 ± 1.1
SUP45 sup45-102 2.2 ± 0.7 4.3 ± 0.9
SUP45 sup45-104 2.5 ± 0.9 12.1 ± 2.2
SUP45 sup45-105 0.3 ± 0.2 15.6 ± 1.7
SUP45 sup45-107 2.0 ± 0.3 5.0 ± 0.6
  1. a Strain 1A-D1628 bearing disruption of the SUP45 gene (SUP45::HIS3) and the SUP45 gene on pRS315 plasmid was transformed with pRS316/sup45-n plasmids. b The frequency of pRS315/SUP45 loss in cotransformants was determined (I). c Transformants bearing only sup45-n plasmids were selected on SC- Ura medium, re-transformed with pRS315/SUP45 plasmid and the frequency of pRS315/SUP45 loss in cotransformants was determined again (II). At least 3 independent transformants were tested for each plasmid combinations. The average data with standard errors (± SE) are presented.