Viable nonsense mutants for the essential gene SUP45 of Saccharomyces cerevisiae

BMC Molecular Biology

Table 1 The efficiency of maintenance of pRS315/SUP45 plasmid on SUP45::HIS3 [sup45-n] background

Transformants of 1A-D1628 bearing plasmids ^a:		% of Ura⁺ Leu^- colonies after rounds of selection:
pRS315 [LEU2] with:	pRS316 [URA3] with:	I ^b	II ^c
SUP45	SUP45	9.5 ± 1.1	11.0 ± 0.7
SUP45	sup45-101	6.3 ± 0.8	10.9 ± 1.1
SUP45	sup45-102	2.2 ± 0.7	4.3 ± 0.9
SUP45	sup45-104	2.5 ± 0.9	12.1 ± 2.2
SUP45	sup45-105	0.3 ± 0.2	15.6 ± 1.7
SUP45	sup45-107	2.0 ± 0.3	5.0 ± 0.6

^a Strain 1A-D1628 bearing disruption of the SUP45 gene (SUP45::HIS3) and the SUP45 gene on pRS315 plasmid was transformed with pRS316/sup45-n plasmids. ^b The frequency of pRS315/SUP45 loss in cotransformants was determined (I). ^c Transformants bearing only sup45-n plasmids were selected on SC- Ura medium, re-transformed with pRS315/SUP45 plasmid and the frequency of pRS315/SUP45 loss in cotransformants was determined again (II). At least 3 independent transformants were tested for each plasmid combinations. The average data with standard errors (± SE) are presented.

ISSN: 1471-2199