Figure 6

(A) Diagram of internal deletions of exons 8 and 9 within constructs expressing full length OXR1 proteins (exons 8–16). Bars represent regions of exon 8 that are missing in the OXR1 deletion mutants. (B) Papillation assay for control plasmid (pBR322), an OXR1 encoded by exons 8–16 (pMV1266), and the five internal deletions of OXR1 diagrammed in part A. Plasmids were expressed in strain MV4709 (mutM mutY lacZ cc104) containing LacI-producing plasmid pMS421. Dilutions and cell platings were as in Figure 2B using 0.125 mM and 1.0 mM IPTG on minimal lactose plates containing 100 μg/ml carbenicillin, 40 μg/ml streptomycin and 20 μg/ml spectinomycin. (C) SDS-PAGE comparing full length OXR1 (pMV1266) and OXR1 deletion mutants described in part A. The expected sizes of the protein fragments from these plasmid are as follows: pMV1266 (38.2 kDa); pKM398 (35.2 kDa); pKM406 (33.1 kDa); pKM407 (36.5 kDa) pKM394 (36.8 kDa); pKM361 (35.5 kDa).