Figure 6

BRN2 knockdown. HEK293 cells were transfected with scrambled (SC) or BRN2 (BRN2.1 or BRN2.2) siRNAs. A) Q-PCR analysis for BRN2 to confirm efficient BRN2 knockdown. BRN2 was amplified using the primers shown in Table 2. B) Western blot analysis of BRN2 expression to confirm efficient BRN2 knockdown. C) Gel shift analysis for BRN2 specific band shift. ³²P-labeled UP (Table 1 and Fig. 4) was added to nuclear extracts (5 μg) prepared from HEK293 cells transfected with scrambled (SC) or BRN2 (BRN2.1 or BRN2.2) siRNAs. Arrowheads indicate significant band shifts.