Figure 1

FABP7 expression in RCC cell lines. A) Q-PCR analysis was performed using total RNA extracted from a human embryonic kidney (HEK293) and RCC (TUHR14TKB, OS-RC-2, 786-O, 769-P, Caki-1, and ACHN) cell lines. FABP7 expression was determined with Q-PCR by the primers listed in Table 2. B) HEK293 and RCC (TUHR14TKB, OS-RC-2, 786-O, 769-P, Caki-1, and ACHN) cells were cotransfected with the -1122+89 pGL4-FABP7 promoter construct, and cultured for one day. Extracts prepared from transfected cells were assayed for luciferase activity. FABP7 promoter activities were normalized to the control's Renilla luciferase activity. The results shown are an average of four independent experiments with standard deviations indicated by the error bars. C) Western blot analysis was performed using cytoplasmic extracts from HEK293 and RCC (TUHR14TKB, OS-RC-2, 786-O, 769-P, Caki-1, and ACHN) cell lines.