Figure 5From: Chibby forms a homodimer through a heptad repeat of leucine residues in its C-terminal coiled-coil motifGlutaraldehyde cross-linking of dimerization-deficient Cby mutants. His-tagged CbyWT, L77A/L91A or 4A was transiently expressed in HEK293T cells, and purified using Ni-NTA beads. The purified Cby protein was incubated in the absence or presence of glutaraldehyde for 5 min, and resolved on SDS-PAGE, followed by immunoblotting with anti-Cby antibody.Back to article page