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Fig. 8 | BMC Molecular Biology

Fig. 8

From: MicroRNA-325-3p protects the heart after myocardial infarction by inhibiting RIPK3 and programmed necrosis in mice

Fig. 8

Influences of RIPK3 dysregulation on the viability of cardiomyocytes in vitro. a RIPK3-targeting siRNA increased the cell viability of cardiomyocytes under hypoxic conditions. b The protein expression of RIPK1, RIPK3 and p-MLKL in cardiomyocytes affected by RIPK3-targeting siRNA. Representative images (top) and quantitative comparison (bottom) are shown. **P < 0.01 compared to cells cultured under normal conditions, #P < 0.05 and ##P < 0.01 compared to cells treated with hypoxia + siRNA-control + Z-IETD-FMK. MI, myocardial infarction; antagomiR-325-3p, miR-325-3p antagomir; siRIPK3, a RIPK3-targeting small interfering RNA; siRNA-control, scrambled siRNA control; Z-IETD-FMK, a caspase inhibitor, benzyloxycarbonyl (Cbz)-Ile-Glu (OMe)-Thr-Asp (OMe)-FMK; RIPK1, receptor-interacting serine/threonine protein kinase 1; RIPK3, receptor-interacting serine/threonine protein kinase 3; p-MLKL, phosphorylated mixed-lineage kinase domain-like protein

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