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Table 3 Expression stability of the nine candidate reference genes, as calculated by BestKeeper

From: Selection of reference genes for the quantitative real-time PCR normalization of gene expression in Isatis indigotica fortune

Rank ABA treatment MeJA treatment N treatment Different tissues All samples
Gene CV ± SD Gene CV ± SD Gene CV ± SD Gene CV ± SD Gene CV ± SD
1 MUB 1.31 ± 0.29 TIP41 1.05 ± 0.25 CYP 0.29 ± 0.05 ACT 1.62 ± 0.34 MUB 3.21 ± 0.72
2 TIP41 1.45 ± 0.34 MUB 1.49 ± 0.35 TIP41 0.29 ± 0.06 MUB 1.66 ± 0.35 ACT 3.52 ± 0.80
3 ACT 1.72 ± 0.40 EF1-α 1.56 ± 0.30 MUB 0.32 ± 0.07 CYP 2.96 ± 0.60 TIP41 3.78 ± 0.85
4 CYP 2.42 ± 0.53 α-TUB 1.99 ± 0.44 EF1-α 0.47 ± 0.08 α-TUB 3.15 ± 0.63 α-TUB 4.99 ± 1.08
5 EF1-α 2.50 ± 0.47 ACT 2.30 ± 0.53 ACT 0.94 ± 0.21 TIP41 3.24 ± 0.69 UBC 4.96 ± 1.18
6 UBC 3.22 ± 0.78 CYP 2.45 ± 0.55 β-TUB 0.97 ± 0.24 EF1-α 3.67 ± 0.60 CYP 5.29 ± 1.12
7 α-TUB 4.20 ± .94 RPL 2.85 ± 0.62 UBC 1.18 ± 0.27 RPL 4.19 ± 0.78 EF1-α 5.69 ± 1.03
8 RPL 4.30 ± 0.89 β-TUB 2.88 ± 0.75 RPL 1.85 ± 0.41 β-TUB 4.32 ± 0.95 β-TUB 5.50 ± 1.35
9 β-TUB 4.40 ± 1.11 UBC 7.33 ± 1.83 α-TUB 2.08 ± 0.47 UBC 5.27 ± 1.22 RPL 6.38 ± 1.32