Table 3 Details of candidate reference genes and primers used for RT-qPCR studies in Thrips tabaci
Gene symbol | Locus description | Homolog locus | Primer sequence (5′→3′) | Identity | E-value |
|---|---|---|---|---|---|
ACTIN | Actin-muscle-specific | KMQ83754 | Fwd: CCCTCCACCATCAAGATCAA Rev: AGATCCACATGGACTGGAA | 97 | 7.69437E−38 |
18s | 18s rRNA | XP_015126904 | Fwd: CTCGAAATGCTCGAGGAAAG Rev: GAATCAGGACGTGTCTCTAACC | 89 | 3.3679E−150 |
28s | 28s ribosomal protein S29, mitochondrial | KDR19525 | Fwd: GAGGGATGGGAACACATTG Rev: AAGCGCCGATCTATGTAGAAG | 65 | 8.4952E−123 |
TUB | Tubulin gamma-2 chain | KDQ97903 | Fwd: GGTTGATCCCACTCAAGTTC Rev: GGTGATTTGCGAGATAGAGC | 95 | 0 |
GADPH | Glyceraldehyde-3-phosphate dehydrogenase 2 | XP_014230283 | Fwd: GAGGTTGTGTCCTCTGACTT GCCATACTCATTGTCGTACC | 95 | 0 |
EF | transcription elongation factor SPT5, putative | XP_002428957 | Fwd: GGACCTTACACTCCACAAAC Rev: GGACCCTTGATAACCTGTTG | 81 | 0 |
RPL17 | 60S ribosomal protein L17 | KDR19552 | Fwd: CACATCGAAGTAGTGCTGAC Rev: GTTTGGCGAGCTTCTTCTTG | 95 | 2.44944E−82 |
Hist3 | Histone-lysine N-methyltransferase, H3 lysine-9 specific 5 | KDR11973 | Fwd: CAGGACAGCGAACTTATGAC Rev: CCATCTGATCCCTGATGTGT | 65 | 0 |
UbiCE | ubiquitin conjugating enzyme | XP_954044 | Fwd: ACCCAAACATAGGACTGTCG Rev: TGGATCAGCTAGGAGAGACT | 53 | 1.31285E−26 |
TATA | TATA box-binding protein-like protein 1 | KDR19315 | Fwd: ACGTGGACTCAAGGATAGTG Rev: TCCCAGTCTTCATCATCTGC | 88 | 3.6384E−116 |
E2F | Transcription factor E2F1 | XP_012270447 | Fwd: GCCGATTAAACCTGGAGTCT Rev: GGGCTACCATATGAGCTGTT | 60 | 8.7238E−111 |
vATPase | V-ATPase holoenzyme subunit B | AJM13638 | Fwd: CCAGGAAAGACCACTCTGAT Rev: GTCAATGCCTCTTCACCAAC | 99 | 0 |
SOD | Mn superoxide dismutase | AIG92784 | Fwd: CAAGGCAGTTGGTGTTCAAG Rev: TGCAGAGGATCTTGGTTAGC | 75 | 5.58161E−69 |
GSTD2 | Glutathione s-transferase D2 | AFJ75818 | Fwd: GTTTCAAGAGCGTCGTCAAC Rev: GACACACTCACACACACTCA | 79 | 1.3299E−84 |