Table 2 Comprehensive ranking of candidate reference genes in different stages of insect and under starvation stress using RefFinder

Adult

 Method

1

2

3

4

5

6

7

8

9

10

 DELTA CT

UbiCE

28s

GSTD2

TATA

SOD

RPL17

TUB

18s

EF

E2F

 BestKeeper

RPL17

TATA

EF

E2F

UBICE

28s

GSTD2

TUB

SOD

18s

 Normfinder

UbiCE

28s

GSTD2

TATA

RPL17

SOD

TUB

18s

E2F

EF

 Genorm

18s|TUB

SOD

28s

GSTD2

UbiCE

TATA

RPL17

EF

E2F

 Recommended comprehensive ranking

UbiCE

28s

TATA

RPL17

GSTD2

TUB

18s

SOD

EF

E2F

Nymph

 Method

1

2

3

4

5

6

7

8

9

10

 Delta CT

SOD

GSTD2

18s

E2F

RPL17

28s

TUB

TATA

UbiCE

EF

 BestKeeper

UbiCE

TATA

E2F

GSTD2

SOD

TUB

18s

28s

RPL17

EF

 Normfinder

SOD

GSTD2

18s

E2F

TUB

RPL17

28s

TATA

UbiCE

EF

 Genorm

SOD|GSTD2

18s

28s

RPL17

E2F

TUB

TATA

UbiCE

EF

 Recommended comprehensive ranking

SOD

GSTD2

18s

E2F

UbiCE

TATA

28s

RPL17

TUB

EF

Starvation stress

 Method

1

2

3

4

5

6

7

8

9

10

 Delta CT

TUB

18s

UbiCE

EF

GSTD2

E2F

TATA

RPL17

28s

SOD

 BestKeeper

TUB

E2F

EF

TATA

18s

UbiCE

GSTD2

28s

RPL17

SOD

 Normfinder

18s

TUB

UbiCE

EF

GSTD2

E2F

RPL17

TATA

28s

SOD

 Genorm

18s|TUB

UbiCE

EF

E2F

TATA

GSTD2

RPL17

28s

SOD

 Recommended comprehensive ranking

TUB

18s

UbiCE

EF

E2F

GSTD2

TATA

RPL17

28s

SOD

Overall analysis

 Method

1

2

3

4

5

6

7

8

9

10

 Delta CT

GSTD2

18s

28s

SOD

TUB

E2F

EF

RPL17

TATA

UbiCE

 BestKeeper

TUB

18s

RPL17

SOD

GSTD2

28s

UbiCE

TATA

E2F

EF

 Normfinder

GSTD2

18s

28s

SOD

TUB

E2F

EF

RPL17

TATA

UbiCE

 Genorm

SOD| GSTD2

18s

28s

E2F

EF

TUB

RPL17

TATA

UbiCE

 Recommended comprehensive ranking

GSTD2

18s

SOD

TUB

28s

RPL17

E2F

EF

TATA

UbiCE