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Table 2 Comprehensive ranking of candidate reference genes in different stages of insect and under starvation stress using RefFinder

From: RNA sequencing, selection of reference genes and demonstration of feeding RNAi in Thrips tabaci (Lind.) (Thysanoptera: Thripidae)

Adult
 Method 1 2 3 4 5 6 7 8 9 10
 DELTA CT UbiCE 28s GSTD2 TATA SOD RPL17 TUB 18s EF E2F
 BestKeeper RPL17 TATA EF E2F UBICE 28s GSTD2 TUB SOD 18s
 Normfinder UbiCE 28s GSTD2 TATA RPL17 SOD TUB 18s E2F EF
 Genorm 18s|TUB   SOD 28s GSTD2 UbiCE TATA RPL17 EF E2F
 Recommended comprehensive ranking UbiCE 28s TATA RPL17 GSTD2 TUB 18s SOD EF E2F
Nymph
 Method 1 2 3 4 5 6 7 8 9 10
 Delta CT SOD GSTD2 18s E2F RPL17 28s TUB TATA UbiCE EF
 BestKeeper UbiCE TATA E2F GSTD2 SOD TUB 18s 28s RPL17 EF
 Normfinder SOD GSTD2 18s E2F TUB RPL17 28s TATA UbiCE EF
 Genorm SOD|GSTD2   18s 28s RPL17 E2F TUB TATA UbiCE EF
 Recommended comprehensive ranking SOD GSTD2 18s E2F UbiCE TATA 28s RPL17 TUB EF
Starvation stress
 Method 1 2 3 4 5 6 7 8 9 10
 Delta CT TUB 18s UbiCE EF GSTD2 E2F TATA RPL17 28s SOD
 BestKeeper TUB E2F EF TATA 18s UbiCE GSTD2 28s RPL17 SOD
 Normfinder 18s TUB UbiCE EF GSTD2 E2F RPL17 TATA 28s SOD
 Genorm 18s|TUB   UbiCE EF E2F TATA GSTD2 RPL17 28s SOD
 Recommended comprehensive ranking TUB 18s UbiCE EF E2F GSTD2 TATA RPL17 28s SOD
Overall analysis
 Method 1 2 3 4 5 6 7 8 9 10
 Delta CT GSTD2 18s 28s SOD TUB E2F EF RPL17 TATA UbiCE
 BestKeeper TUB 18s RPL17 SOD GSTD2 28s UbiCE TATA E2F EF
 Normfinder GSTD2 18s 28s SOD TUB E2F EF RPL17 TATA UbiCE
 Genorm SOD| GSTD2   18s 28s E2F EF TUB RPL17 TATA UbiCE
 Recommended comprehensive ranking GSTD2 18s SOD TUB 28s RPL17 E2F EF TATA UbiCE