A fragment activity assay reveals the key residues of TBC1D15 GTPase-activating protein (GAP) in Chiloscyllium plagiosum

Table 1 The catalytic efficiency parameter (k_cat/K_m) values of Shark-/Sus-/Homo-TBC1D15-GAP/GAP mutants

Fragments	Rab4	Rab5	Rab7	Rab11
Shark-GAP	113.8 ± 20.5^bA	212.8 ± 43.8^aA	90.9 ± 20.4^bcA	94.8 ± 13.5^bcB
Shark-GAP-mut	52.8 ± 10.5^bB	56.1 ± 4.78^bB	53.3 ± 9.47^bB	130.6 ± 20.5^aA
Sus-GAP	56.9 ± 24.7^bB	72 ± 13.6^abB	56.3 ± 17.5^bB	105 ± 26.6a^B
Sus-GAP-mut	39.9 ± l7.02^bB	57.6 ± 12.5^bB	56.3 ± 6.18^bB	105 ± l 7.4^aB
Homo-GAP	60.6 ± 16.l3^aB	69.7 ± 17.63^aB	55.1 ± 9.47^aB	74.9 ± 15.7^aB
Homo-GAP-mut	68.3 ± 16.5^aB	61.1 ± 16.4^aB	55.6 ± 5.3^aB	66.8 ± 27.5^aB

The results showed above are mean values ± SD from three independent experiments. Different uppercase letters indicate significant (P < 0.05) differences among values within a column. Different lowercase letters indicate significant (P < 0.05) differences among values in a row

ISSN: 1471-2199