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Table 1 The catalytic efficiency parameter (kcat/Km) values of Shark-/Sus-/Homo-TBC1D15-GAP/GAP mutants

From: A fragment activity assay reveals the key residues of TBC1D15 GTPase-activating protein (GAP) in Chiloscyllium plagiosum

Fragments Rab4 Rab5 Rab7 Rab11
Shark-GAP 113.8 ± 20.5bA 212.8 ± 43.8aA 90.9 ± 20.4bcA 94.8 ± 13.5bcB
Shark-GAP-mut 52.8 ± 10.5bB 56.1 ± 4.78bB 53.3 ± 9.47bB 130.6 ± 20.5aA
Sus-GAP 56.9 ± 24.7bB 72 ± 13.6abB 56.3 ± 17.5bB 105 ± 26.6aB
Sus-GAP-mut 39.9 ± l7.02bB 57.6 ± 12.5bB 56.3 ± 6.18bB 105 ± l 7.4aB
Homo-GAP 60.6 ± 16.l3aB 69.7 ± 17.63aB 55.1 ± 9.47aB 74.9 ± 15.7aB
Homo-GAP-mut 68.3 ± 16.5aB 61.1 ± 16.4aB 55.6 ± 5.3aB 66.8 ± 27.5aB
  1. The results showed above are mean values ± SD from three independent experiments. Different uppercase letters indicate significant (P < 0.05) differences among values within a column. Different lowercase letters indicate significant (P < 0.05) differences among values in a row