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Fig. 3 | BMC Molecular Biology

Fig. 3

From: Matrix association region/scaffold attachment region (MAR/SAR) sequence: its vital role in mediating chromosome breakages in nasopharyngeal epithelial cells via oxidative stress-induced apoptosis

Fig. 3

Cleavage frequencies of the AF9 SAR and non-SAR regions in H2O2-treated HK1 cells. a Representative gel pictures showing the IPCR results of the AF9 gene in H2O2-treated HK1 cells: i SAR region, ii non-SAR region. HK1 cells were either untreated (lanes 2–6) or treated with 50 µM of H2O2 for 8 h (lanes 7–11). The cells were harvested for gDNA extraction and nested IPCR. For each cell sample, five IPCR replicates (R1–R5) were prepared. The side brackets show the IPCR bands derived from the cleavages of the AF9 gene. M: 100 bp DNA ladder. N: Negative control for IPCR. b AF9 cleavage frequency detected in HK1 cells. Data are representative of two independent experiments. Each experiment consisted of 1–2 sets of IPCR. Each set of IPCR was performed in 5–6 IPCR replicates per cell sample. The results are expressed as medians with IQRs. *P < 0.05; NS: no significant difference (Mann–Whitney U test)

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