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Table 1 Loss and recovery of twm1 antisense transformant mtDNA following EtBr exposure

From: The Dictyostelium discoideum homologue of Twinkle, Twm1, is a mitochondrial DNA helicase, an active primase and promotes mitochondrial DNA replication

  TAS vector copy number Initial mtDNA (%) relative to AX2 mtDNA loss (%) after 24 h mtDNA recovery (%) after 24 h
AX2 0 100.00 53.02 ± 2.94 49.34 ± 3.83
TAS #22 25 86.56 ± 3.98 59.03 ± 3.93 30.30 ± 2.89
TAS #7 73 78.02 ± 3.30 71.48 ± 4.37 21.40 ± 4.17
TAS #15 100 66.89 ± 4.21 65.67 ± 2.51 25.68 ± 4.86
TAS #27 112 59.56 ± 3.71 69.56 ± 2.09 29.86 ± 3.78
TAS #20 115 68.31 ± 4.84 62.04 ± 2.26 36.31 ± 2.53
TAS #9 163 39.97 ± 3.81 73.30 ± 4.29 25.75 ± 4.58
  1. Parental AX2 and twm1 antisense transformants (TAS) were exposed to EtBr for 24 h, and subsequently allowed a further 24 h to recover. Relative mtDNA copy number was determined using qPCR and the single copy tubB nuclear gene. Standard error is provided. mtDNA loss and recovery percentages are relative to each strain’s initial mtDNA copy number