The Dictyostelium discoideum homologue of Twinkle, Twm1, is a mitochondrial DNA helicase, an active primase and promotes mitochondrial DNA replication

Table 1 Loss and recovery of twm1 antisense transformant mtDNA following EtBr exposure

	TAS vector copy number	Initial mtDNA (%) relative to AX2	mtDNA loss (%) after 24 h	mtDNA recovery (%) after 24 h
AX2	0	100.00	53.02 ± 2.94	49.34 ± 3.83
TAS #22	25	86.56 ± 3.98	59.03 ± 3.93	30.30 ± 2.89
TAS #7	73	78.02 ± 3.30	71.48 ± 4.37	21.40 ± 4.17
TAS #15	100	66.89 ± 4.21	65.67 ± 2.51	25.68 ± 4.86
TAS #27	112	59.56 ± 3.71	69.56 ± 2.09	29.86 ± 3.78
TAS #20	115	68.31 ± 4.84	62.04 ± 2.26	36.31 ± 2.53
TAS #9	163	39.97 ± 3.81	73.30 ± 4.29	25.75 ± 4.58

Parental AX2 and twm1 antisense transformants (TAS) were exposed to EtBr for 24 h, and subsequently allowed a further 24 h to recover. Relative mtDNA copy number was determined using qPCR and the single copy tubB nuclear gene. Standard error is provided. mtDNA loss and recovery percentages are relative to each strain’s initial mtDNA copy number

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