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Fig. 9 | BMC Molecular Biology

Fig. 9

From: The Dictyostelium discoideum homologue of Twinkle, Twm1, is a mitochondrial DNA helicase, an active primase and promotes mitochondrial DNA replication

Fig. 9

In bacterio replication of pZErO-2 by D. discoideum Twm1. E. coli BL21 strains each possessed two vectors; pET-23a:tetA either as an empty vector (−) or encoding Twm1 (+), and pZErO-2 with (+) or without (−) the NCRrnl or rnl region of the D. discoideum mitochondrial genome. Twm1 expression was induced by the addition of 1 mM IPTG. Relative pZErO-2 vector copy number (using primers for kanR) was quantified using qPCR against a single copy number bacterial gene, talB. Error bars represent the standard error. p values were calculated using Student’s t-test (*< 0.05; **< 0.001)

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