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Table 1 PCR Primers utilized to amplify FOXO3 gene fragments for Donor Vector Gibson Assembly Reactions

From: A protocol for custom CRISPR Cas9 donor vector construction to truncate genes in mammalian cells using pcDNA3 backbone

Primer Sequencea
FOXO3 ARM 1 F 5′-GTGCTTTACGGCACCTCGACCCCCCGGCACAACCTGTCACTGC-3′
FOXO3 ARM 1 R 5′-CCGTCTATCAGGGCGATGGCCGCTGTAGAGCATGGGCGAGAG-3′
FOXO3 ARM 2 F 5′-CAAATAAAGCATTTTTTTCACTCGGTGGAACTGCCACGGCTG-3′
FOXO3 ARM 2 R 5′-GAGTTTGGACAAACCACAACTAGGTCCAAGTCGCTGGGGAAC-3′
  1. aNucleotides in bold corresponds to pcDNA3 vector sequences. Sequences in italics were to amplify the indicated FOXO3 arm