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Fig. 4 | BMC Molecular Biology

Fig. 4

From: An optimised protocol for isolation of RNA from small sections of laser-capture microdissected FFPE tissue amenable for next-generation sequencing

Fig. 4

Comparison of Next-Generation Sequencing results obtained with RNA isolated with the old and new method, respectively. a Barplot illustrating the relative amount of mapped (blue) vs. unmapped (grey) reads using the new protocol (left) or the old protocol (right). b Barplot showing fractions of mapped reads specific for exonic (blue), intronic (light blue) and other genomic (grey) regions. c Linegraph illustrating the calculated read-coverage over the genebody for expressed genes of medium length (400–1000 nt). Results for samples extracted by the new protocol are highlighted in red colors

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