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Fig. 2 | BMC Molecular Biology

Fig. 2

From: RUNX1 induces DNA replication independent active DNA demethylation at SPI1 regulatory regions

Fig. 2

DNA methylation status analysis of SPI1 −17-kb URE in RUNX1-overexpressing HEK-293T cells: a Target sequence of human SPI1 −17-kb URE. The number represents the CpG sites and RUNX1 and SPI1 binding sites are shown in bold letters. b Methylation pattern diagrams of wild-type, MCS, and RUNX1-overexpressing HEK-293T cells at the SPI1 −17-kb URE region. Each row of circles represents the result of a single amplicon and each column represents those of a single CpG site. Black circles denote methylated CpGs and open circles represent unmethylated ones. The CpG numbers shown correspond to the target nucleotide sequence, shown in a. c Quantification of percent CpG methylation at the −17-kb URE in wild-type, MCS, and RUNX1-overexpressing HEK-293T cells. The p-value calculated from the comparison between MCS and RUNX1-overexpressing HEK-293T cells was significant (***p < 0.001). d Comparison of methylation status graph at each individual CpG site between MCS and RUNX1-overexpressing HEK-293T cells. All of the CpGs were significantly demethylated (p < 0.005)

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