Fig. 4

STAT1 and IRF1 binding at SOCS1. a RT-qPCR for SOCS1 mRNA in HeLa cells treated with IFNγ for 0 or 6 h. Data are in arbitrary units relative to β-actin levels (log scale). b ChIP-chip maps of STAT1 and IRF1 binding across the SOCS1 locus treated as in a. Black arrowheads indicate TF binding sites of interest, with distances from the TSS (red arrow) in kb. The TF-free −63 kb site (blue) is used as a negative control in c. c ChIP-qPCR analysis of the basal and IFNγ-induced histone modifications or recruitment of the indicated factors. Marks (*^{, †, ‡}) show significant differences (p < 0.05, ANOVA followed by Fisher test) in the indicated comparisons (mean ± SD, n = 3)