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Fig. 5 | BMC Molecular Biology

Fig. 5

From: Methylated site display (MSD)-AFLP, a sensitive and affordable method for analysis of CpG methylation profiles

Fig. 5

Percent methylation levels of CpGs in the MSD-AFLP peak charts. a Percent methylation levels derived from MSRE-PCR. b Typical peak chart from liver DNA (upper) and SssI-treated artificially methylated DNA (lower). c Scatter plot of signal ratios (SR) and percent methylation levels (derived from MSRE-PCR) of 11 CpGs. SR was calculated by dividing the fluorescence intensities derived from MSD-AFLP by that of the SssI-treated artificially methylated DNA. An approximation formula expressing the relationship between the percent methylation levels determined by MSRE-PCR analysis and SR was developed using the Hill equation. d Percent methylation levels of the MSD-AFLP peak charts were determined by substituting SR into the Hill equation

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BMC Molecular Biology

ISSN: 1471-2199

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