Fig. 5

Functional analyses of S-SOX5 binding sites in the human SPAG16L promoter. Left maps of the wild-type human SPAG16L promoter construct containing two putative SOX5 binding sites (P-I & P-II) and the constructs with mutations of the potential SOX5 binding sites. Right, effect of S-SOX5 on the function of the wild-type and mutated SPAG16L promoter. BEAS-2B cells were co-transfected with SPAG16L promoter constructs and either pcDNA3 control or S-SOX5/pcDNA3. Relative luciferase activity, normalized to pGL3 control plasmids co-transfected with pcDNA3 vectors. Values are mean ± S.E. (error bars) of the relative luciferase activity (n = 3). Bars labeled with different letters are significantly different (p < 0.05; two-way ANOVA with Tukey’s HSD test)