Fig. 1

Expression and phosphorylation of SRSF2 P95 and deletion mutants. a Schematic of SRSF2HA ORF showing the primary protein domains removed to create SRSF2 deletion mutants. RRM RNA recognition motif; HNG hinge region; RS arginine/serine-rich domain; NRS nuclear retention signal; HA hemagglutinin tag. Numbers denote amino acids defining the domain boundaries within the full length protein. Location of the P95 amino acid within the hinge region is denoted with a large red arrow. b Western blot of protein isolated from cell lines after 48 h doxycycline induction. c Average HA-tagged protein expression in induced cell lines, normalized to GAPDH expression. Statistics are based on one-way analysis of variance (ANOVA) with comparison to SRSF2^WT cell line (n = 5). d qRT-PCR analysis of HA-tagged SRSF2 mRNA expression, normalized to SRSF2^WT. No statistical significance using one-way ANOVA with comparison to SRSF2^WT (n = 4). e Western blot using anti-HA and anti- phospho-SR antibodies after immunoprecipitation of HA-tagged SRSF2 protein. f Analysis of phospho-SR signal normalized to HA-tagged protein signal. Statistics use one-way ANOVA with comparison to SRSF2^WT (n = 3). p values: *≤0.05; **≤0.01; ***≤0.001; ****≤0.0001