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Table 1 Proteins pulled-down with anti-DEspR mAb 5g12e8 from U87 CSC membranes

From: Confirmation of translatability and functionality certifies the dual endothelin1/VEGFsp receptor (DEspR) protein

Accession

%

#SM

#P

#AAs

Mass

Score

Description

P08670

55.8

36

31

466

53,619

177.59

Vimentin (VIM)

P60709

34.4

11

9

375

41,709

66.79

Actin, cytoplasmic 1 (ACTB)

P68371

41.6

13

12

445

49,799

56.53

Tubulin beta-4B chain (TUBB4B)

P07437

35.1

11

10

444

49,638

47.84

Tubulin beta chain (TUBB)

Q13885

29.0

10

9

445

49,874

42.11

Tubulin beta-2A chain (TUBB2A)

P17931

25.6

7

5

250

26,136

39.26

Galectin-3 (LGALS3)

P09382

49.6

7

6

135

14,706

38.90

Galectin-1 (LGALS1) a

Q13509

25.6

8

8

450

50,400

32.86

Tubulin beta-3 chain (TUBB3)

Q9BUF5

15.7

6

5

446

49,825

21.46

Tubulin beta-6 chain (TUBB6)

P61006

13.0

2

2

207

23,653

12.01

Ras-related protein Rab-8A (RAB8A)

C9J8S3

15.0

2

2

160

18,015

11.01

Ras-related protein Rab-7a (RAB7A)

P61026

6.0

1

1

200

22,526

9.59

Ras-related protein Rab-10 (RAB10)

P51153

5.9

1

1

203

22,759

7.60

Ras-related protein Rab-13 (RAB13)

H0YNE9

6.4

1

1

188

21,854

7.51

Ras-related protein Rab-8B (Fragment) (RAB8B)

G3V2K7

7.2

1

1

153

16,893

5.96

Transmembrane emp24 domain-containing protein 10 (TMED10) a

Q9Y3B3-2

5.3

1

1

188

21,219

4.85

Transmembrane emp24 domain-containing protein 7 (TMED7)

Q9BVK6

3.8

1

1

235

27,260

4.36

Transmembrane emp24 domain-containing protein 9 (TMED9)

F8WBC0

34.4

1

1

32

3501

2.90

Ras-related protein Rap-1b (Fragment) (RAP1B)

  1. Proteins in italic face letters are proteins detected in three independent pull-down experiments
  2. % the percentage of the protein sequence covered by identified peptides; #SM the number of peptide spectrum matches; #P the total number of distinct peptide sequences identified in the protein group; #AAs number of amino acids; Mass, mass in Daltons; Score, the sum of the ion scores of all peptides that were identified
  3. aProteins verified by Western blot analysis of pull-down proteins; Rab-1b was detected in two independent experiments and verified by Western blot analysis (Fig. 2b)
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BMC Molecular Biology

ISSN: 1471-2199

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