Fig. 3
From: A potential role for protein palmitoylation and zDHHC16 in DNA damage response
Inhibition of palmitoylation impaired DNA damage-induced Atm activation. a Western blot analysis showed that inhibition of palmitoyltransferase by 2BP led to a decrease in Atm phosphorylation on S1981. Primary MEFs were pre-treated with 50 μM 2BP for 24 h followed by 1 μM Dox for different periods of time. b Quantitation of phosphorylated Atm at Ser1981 after being normalized to total Atm. Asterisk denoted significant difference (p < 0.05) between control and 2BP treated cells. c 2BP compromised DNA damage-induced Smad1 activation. Cells were treated the same as described in 3A and probed for total and phosphorylated Smad1/5/8 and p53