Fig. 2

Inhibition of palmitoylation by 2BP impaired DNA damage-induced p53 up-regulation and activation. a Western blot analysis showed that 2BP pre-treatment impeded the induction of p53 protein expression, p53 phosphorylation on Ser15, and p21 and Bax protein levels in response to Dox treatment. Primary MEFs were pre-treated with 50 or 100 μM 2BP for 24 h and then stimulated with 1 μM of Dox for different periods of time as indicated. The protein levels of p53, p-p53, p21, Bax, and actin were determined by Western blot analysis. b Relative quantitative PCR results showed that 2BP pre-treatment impeded the induction of Bax and p21 (Cdkn1a) gene expression, but not much of Puma (Bbc3) gene expression. The cells were pre-treated without (−2BP) or with 50 μM 2BP (+2BP) for 24 h and followed by 1 μM Dox treatment for 0, 8 and 16 h. The expression of each gene without 2BP and Dox treatment was set as 1 (as indicated by the horizontal line) and used to compare the expression of the same gene under different stimulated conditions. Error bars represented standard error mean of the repeated experiments. Asterisk denoted significant difference (p < 0.05) between Dox alone and 2BP plus Dox treated samples