Fig. 1

Concept to quantify alternative splice variants. a Key elements of the RT-qPCR based strategy for the relative quantification of splice variants. b Scheme of the TRPM3 mRNA structure with exon 12–14 and the alternatively spliced part of exon 13. Different primer pairs and their location relative to the TRPM3 mRNA are shown as arrows. The length of their amplicons [bp] and their isoform specificity are indicated