Fig. 4

miR-150 affects the expression of proteins involved in cell proliferation and apoptosis. a The mRNA expression of cell cycle-related genes CyclinD1 and p27 in the three C-33A sub-cell lines (control, miR-150, miR-150 inhibitor) was measured by RT-PCR. Mean values ± SD for 3 independent experiments is shown. b The mRNA expression of apoptosis-related genes FASL and BIM in these three sub-cell lines from 3 independent experiments was evaluated by RT-PCR. Mean values ± SD for 3 independent experiments are shown. c The expression of Cyclin D1, p27, GAPDH, and phophorylated pRb (p-pRb) in the sub-cell lines was determined by western blot, and the pixel densities of these proteins were analyzed and presented by histograms (bottom). Mean values ± SD for 3 independent experiments is shown. d The expression of FASL, BIM, and GAPDH in the sub-cell lines was determined by western blot, and the pixel densities of these proteins were analyzed from 3 independent experiments and presented by histograms (bottom)