Fig. 4
From: Genes targeted by the Hedgehog-signaling pathway can be regulated by Estrogen related receptor β
Quantitative PCR validation of the top 15 Hedgehog signaling differentially responsive mRNAs. Hh-signaling differentially responsive genes as determined by RNAseq were confirmed by qPCR after NIH3T3-pc3.1 and NIH3T3-Esrrb cells are treated with Hh-CM. Each mRNA concentration is normalized to housekeeping gene GAPDH, and further normalized to that ratio in NIH3T3-pc3.1. Y-axis represents relative fold change to no Esrrb expressed no Hh-CM treated control. a Esrrb altered the Hh-CM treatment response, due to a proportional additive effect from Esrrb expression. When Esrrb is expressed, the genetic response to Hh is different than the condition without Esrrb expressed, Asterisk means p < 0.01 and b genes respond to Hedgehog signaling better when Esrrb is expressed as well as genes losing Hh response when Esrrb is expressed, Asterisk means p < 0.01 Number sign means “not statistically significant”