Fig. 1
From: Deciphering targeting rules of splicing modulator compounds: case of TG003
TG003 is a general skip enhancer both in human and mouse. a RNA-seq data identifies splicing changes in formerly known TG003 sensitive exons. RNA-seq data was inputted to sashimi-plot for graphical representations. Numbers of junction reads calculated by our method are shown at the junction position. The black boxes below represent the exons and the arrows indicate the direction of transcription. ψ and Δψ values are calculated on the right. b Left histogram of the average Δψ values [Δψavg = (ΨDMSO.rep1 − ΨTG003.rep1 + ΨTG003.rep2 − ΨDMSO.rep2)/2)] of all annotated exon-skipping exons are shown in green for human and yellow for mouse. The distribution of difference in ψ between replicates of the same condition are shown in black to show the average baseline fluctuation of ψ values [(ΨDMSO.rep1 − ΨDMSO.rep2 + ΨTG003.rep1 − ΨTG003.rep2)/2]. The black dashed line shows the cutoff value of Δψ = −0.05 for skip and Δψ = 0.05 for inclusion. Right pie chart of the ratio of skip-enhanced (Δψavg ≤ −0.05) and inclusion-enhanced (Δψavg ≥ 0.05) exons. c Venn diagram of the overlap between the number of human and mouse annotated exon-skipping events in Ensembl. d Distribution of 1145 expressed and consistent conserved annotated exon-skipping events in both species into the nine categories. Circles roughly show the scales, and the numbers show the exact counts. Numbers in the brackets show the expected values for each categories under the assumption of no-conservation of TG003 effects between the species