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Table 2 The Spike-in Qubit™ RNA HS Assay achieves 5 pg/μL lower quantification limit for trophoblast total RNA

From: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in

Expected increase (pg/μL) Ave. measured increase ± SD (pg/μL) Relative error Coefficient of variation Deviation from ideal
1 1.7 ± 0.8 65.8% 50.6% 116.5%
2 2.9 ± 0.7 44.2% 23.9% 68.0%
3 3.0 ± 0.5 0.6% 16.7% 17.3%
4 4.4 ± 0.8 10.6% 18.4% 29.1%
5 5.1 ± 0.8 2.2% 16.3% 18.5%
7.5 7.2 ± 0.7 4.1% 9.6% 13.7%
10 9.7 ± 1.0 2.6% 10.5% 13.0%
15 14.5 ± 0.8 3.6% 5.2% 8.8%
20 19.3 ± 1.0 3.7% 5.3% 9.0%
  1. A 250 pg/μL trophoblast total RNA sample was used to assess the accuracy and precision of reading increases between 1 and 20 pg/μL using the Spike-in Qubit™. Average measured reading increase ± standard deviation (SD), relative error, coefficient of variation, and deviation from ideal expressed in percent are listed in the table. N = 4 independent repeats.