Table 2 The Spike-in Qubit™ RNA HS Assay achieves 5 pg/μL lower quantification limit for trophoblast total RNA
From: Lowering the quantification limit of the QubitTM RNA HS Assay using RNA spike-in
Expected increase (pg/μL) | Ave. measured increase ± SD (pg/μL) | Relative error | Coefficient of variation | Deviation from ideal |
|---|---|---|---|---|
1 | 1.7 ± 0.8 | 65.8% | 50.6% | 116.5% |
2 | 2.9 ± 0.7 | 44.2% | 23.9% | 68.0% |
3 | 3.0 ± 0.5 | 0.6% | 16.7% | 17.3% |
4 | 4.4 ± 0.8 | 10.6% | 18.4% | 29.1% |
5 | 5.1 ± 0.8 | 2.2% | 16.3% | 18.5% |
7.5 | 7.2 ± 0.7 | −4.1% | 9.6% | 13.7% |
10 | 9.7 ± 1.0 | −2.6% | 10.5% | 13.0% |
15 | 14.5 ± 0.8 | −3.6% | 5.2% | 8.8% |
20 | 19.3 ± 1.0 | −3.7% | 5.3% | 9.0% |